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FunGene: 08-OCT-2024

Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001977
  • pan locus tag?: SAUPAN005192000
  • symbol: lukA
  • pan gene symbol?: lukH
  • synonym:
  • product: bi-component leukocidin LukGH subunit H

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001977
  • symbol: lukA
  • product: bi-component leukocidin LukGH subunit H
  • replicon: chromosome
  • strand: -
  • coordinates: 1989583..1990635
  • length: 1053
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAATAAAAAACGTGTTTTAATAGCGTCATCATTATCATGTGCAATTTTATTGTTA
    TCAGCAGCAACGACTCAAGCAAATTCAGCTCATAAAGACTCGCAAGACCAAAATAAGAAA
    GAACATGTTGATAAGTCTCAACAAAAAGAGAAACGTAATGTTACTAATAAAGATAAAAAT
    TCAACAGCACCGGATGATATTGGGAAAAACGGTAAAATCACAAAACGAACTGAAACAGTA
    TATGATGAGAAAACAAATATACTCCAAAATTTACAATTCGACTTTATCGATGATCCAACT
    TATGACAAGAATGTATTACTTGTTAAAAAACAAGGCTCAATTCATTCAAATTTAAAGTTT
    GAATCTCATAAAGAAGAAAAAAATTCAAATTGGTTAAAGTATCCAAGTGAGTACCATGTA
    GATTTTCAAGTAAAAAGAAATCCTAAAACTGAAATATTAGACCAATTGCCGAAAAATAAA
    ATTTCAACTGCGAAAGTAGACAATACATTTTCATATAGCTCAGGTGGTAAATTCGATTCA
    ACGAAAGGTATTGGACGAACTTCATCAAATAGCTACTCCAAAACGATTAGTTATAATCAG
    CAAAATTATGACACAATTGCCAGTGGTAAAAATAATAACTGGCATGTACACTGGTCAGTT
    ATTGCGAATGACTTGAAGTATGGTGGAGAAGTGAAAAATAGAAATGATGAATTATTATTC
    TATAGAAATACGAGAATTGCTACTGTAGAAAACCCTGAACTAAGCTTTGCTTCAAAATAT
    AGATACCCAGCATTAGTAAGAAGTGGCTTTAATCCAGAATTTTTAACTTATTTATCTAAT
    GAAAAGTCAAATGAGAAAACGCAATTTGAAGTAACATACACACGAAATCAAGATATTTTG
    AAAAACAGACCTGGAATACATTATGCACCTCCAATTTTAGAAAAAAATAAAGAAGGTCAA
    AGATTAATTGTCACTTATGAAGTTGATTGGAAAAATAAAACAGTTAAAGTCGTTGATAAA
    TACTCTGATAATAAATCATTTAGAGAAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: JSNZ_001977
  • symbol: LukA
  • description: bi-component leukocidin LukGH subunit H
  • length: 350
  • theoretical pI: 9.97603
  • theoretical MW: 40316.7
  • GRAVY: -1.03257

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 190.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 272.3)
    and 4 more
    DUF1214 (CL0528) DUF1214; Protein of unknown function (DUF1214) (PF06742; HMM-score: 14.2)
    no clan defined GRP; Glycine rich protein family (PF07172; HMM-score: 13.5)
    Omega_toxin (CL0083) Conotoxin; Conotoxin (PF02950; HMM-score: 13)
    no clan defined PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 7.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.9992
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.768673
    • TAT(Tat/SPI): 0.000885
    • LIPO(Sec/SPII): 0.229785
    • Cleavage Site: CS pos: 29-30. ANS-AH. Pr: 0.3420
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

Protein sequence[edit | edit source]

  • MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVDKSQQKEKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYDKNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNKISTAKVDNTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHWSVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLTYLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKTVKVVDKYSDNKSFREG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, ArcR* (activation) regulon, SaeR (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    ArcR*(TF)important in Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

Literature[edit | edit source]

References[edit | edit source]

  1. Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. 2.0 2.1 2.2 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]