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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001976
- pan locus tag?: SAUPAN005190000
- symbol: lukB
- pan gene symbol?: lukG
- synonym:
- product: bi-component leukocidin LukGH subunit G
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001976
- symbol: lukB
- product: bi-component leukocidin LukGH subunit G
- replicon: chromosome
- strand: -
- coordinates: 1988545..1989561
- length: 1017
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGATTAAACAACTATATAAAAATATCACAATTTGTACGTTAGCACTATCGACTACTTTC
ACTGTATTACCAGCTACTTCTTATGCAAAAATTAATTCTGAAATTAAAGCTGTTTCTGAG
AAGAATCTTGATGGTGATACTAAAATGTATACACGTACAGCTACAACAAGTGATAGTCAA
AAAAATATTACTCAAAGCTTACAATTTAACTTCTTAACTGAACCTAATTATGATAAAGAA
ACAGTATTTATTAAAGCGAAAGGAACAATAGGTAGTGGTTTAAGAATTCTAGACCCAAAT
GGTTATTGGAATAGTACATTAAGATGGCCTGGATCTTATTCAGTTTCAATTCAAAATGTT
GATGACAACAACAATATAAATGTGACTGACTTTGCACCAAAAAATCAGGATGAATCAAGA
GAAGTTAAATATACGTATGGTTATAAAACAGGTGGAGATTTTTCGATTAATCGTGGAGGG
TTAACTGGAAATATTACAAAAGAGAGTAATTATTCAGAGACGATTAGTTATCAACAACCA
TCGTATCGTACATTACTTGATCAATCTACGTCACATAAAGGTGTAGGTTGGAAAGTAGAA
GCACATTTGATAAATAATATGGGACATGACCATACGAGACAATTAACTAATGATAGTGAT
AATAGAACTAAAAGTGAAATCTTTTCTTTAACACGAAATGGAAATTTATGGGCGAAAGAT
AATTTTACGCCTAAGAATAAGATGCCTGTAACTGTTTCTGAAGGGTTTAATCCAGAATTT
TTAGCTGTTATGTCACATGATAAAAAAGATGAAGGTAAATCAAAATTTGTTGTTCATTAT
AAAAGATCAATGGATGAGTTTAAAATAGATTGGAATCGTCATGGTTTCTGGGGCTATTGG
TCTGGTGAAAACCATGTAGATAAAAAAGAAGAAAAATTATCAGCATTATATGAAGTTGAT
TGGAAGACACATAATGTGAAGTTTGTAAAAGTACTTAATGATAATGAAAAGAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001976
- symbol: LukB
- description: bi-component leukocidin LukGH subunit G
- length: 338
- theoretical pI: 9.17801
- theoretical MW: 38716.9
- GRAVY: -0.838166
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 262)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 307.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.73
- Internal Helices: 0
- DeepLocPro: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.9999
- LocateP:
- SignalP: Signal peptide SP(Sec/SPI) length 29 aa
- SP(Sec/SPI): 0.984323
- TAT(Tat/SPI): 0.00438
- LIPO(Sec/SPII): 0.00418
- Cleavage Site: CS pos: 29-30. SYA-KI. Pr: 0.8417
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLDGDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGYWNSTLRWPGSYSVSIQNVDDNNNINVTDFAPKNQDESREVKYTYGYKTGGDFSINRGGLTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTNDSDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKFVVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLNDNEKK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Regulation[edit | edit source]
- regulators: Rex* (repression) regulon, ArcR* (activation) regulon, SaeR (activation) regulon
Rex* (TF) important in Energy metabolism; regulation predicted or transferred from N315 and NCTC 8325 [2] ArcR* (TF) important in Arginine degradation; regulation predicted or transferred from N315 and NCTC 8325 [2] SaeR (TF) important in Virulence; regulation predicted or transferred from N315 and NCTC 8325 [2]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p) - ↑ 2.0 2.1 2.2 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)