From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001817
  • pan locus tag?: SAUPAN004583000
  • symbol: lukD
  • pan gene symbol?: lukD
  • synonym:
  • product: bi-component leukocidin LukED subunit D

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001817
  • symbol: lukD
  • product: bi-component leukocidin LukED subunit D
  • replicon: chromosome
  • strand: -
  • coordinates: 1862227..1863210
  • length: 984
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATGAAAAAATTAATCAAATCATCAGTTGCTTCATCAATTGCACTGCTTTTGCTA
    TCGAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGCGAGAAAAAAGTAGATGAC
    AAAATCACTTTATACAAAACAACAGCAACATCTGATAATGATAAATTGAATATTTCTCAA
    ATTTTAACGTTTAATTTCATTAAGGATAAAAGTTATGACAAAGATACGTTAGTACTTAAG
    GCAGCCGGAAACATTAATTCAGGTTATAAAAAGCCTAATCCAAAAGATTACAATTACTCA
    CAGTTTTATTGGGGCGGTAAGTATAATGTTTCGGTTAGTTCAGAATCAAATGATGCTGTA
    AATGTTGTTGACTATGCACCTAAAAATCAAAATGAAGAATTCCAAGTTCAACAAACATTA
    GGTTATTCTTATGGCGGAGATATTAATATATCTAATGGCTTATCAGGTGGATTAAATGGA
    TCAAAATCATTTTCAGAAACGATAAATTATAAACAAGAAAGTTACAGAACTACGATTGAT
    AGAAAAACAAATCATAAATCAATTGGCTGGGGTGTTGAGGCGCACAAAATTATGAATAAT
    GGTTGGGGACCATATGGTAGAGATAGTTATGACCCAACATATGGTAATGAACTGTTTTTA
    GGTGGTAGACAAAGTAGTTCAAATGCTGGACAAAATTTCTTGCCAACACATCAAATGCCT
    TTATTGGCGCGTGGTAACTTTAACCCAGAATTTATAAGCGTACTTTCTCATAAACAAAAT
    GATACAAAAAAATCTAAAATCAAAGTAACTTACCAAAGAGAAATGGATAGATATACTAAT
    CAATGGAATCGATTGCACTGGATTGGTAATAACTACAAAAATCAAAATACAGTAACGTTT
    ACATCTACTTATGAAGTTGATTGGCAAAACCATACTGTTAAATTAATCGGTACGGATTCT
    AAAGAAACTAATCCTGGAGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001817
  • symbol: LukD
  • description: bi-component leukocidin LukED subunit D
  • length: 327
  • theoretical pI: 9.65379
  • theoretical MW: 36916.7
  • GRAVY: -0.813761

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 441.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 328.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.9998
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.995497
    • TAT(Tat/SPI): 0.000381
    • LIPO(Sec/SPII): 0.003073
    • Cleavage Site: CS pos: 26-27. VDA-AQ. Pr: 0.9631
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKMKKLIKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001817&oldid=107458"