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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001758
  • pan locus tag?: SAUPAN004456000
  • symbol: ribB
  • pan gene symbol?: ribA
  • synonym:
  • product: 3,4-dihydroxy-2-butanone-4-phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001758
  • symbol: ribB
  • product: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1811027..1812208
  • length: 1182
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAAACAATTATT
    GTAGTAGATGATGAGAATCGTGAAAATGAAGGTGATTTAGTAGCGGTCACTGAATGGATG
    AACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGGGATTAATATGCGCACCAGTG
    TCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
    TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGTGCT
    TATGAACGTACATTGACTGCCAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTT
    AATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATAAAGGCGTATTAGCTAGAAAT
    GGACACACAGAAGCGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
    ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAAGTTT
    AAAGAAAAACATCAATTAAAGATGATTACGATTGATGATTTAATTGAATATCGTAAAAAA
    TTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGCCTACAGATTTCGGAACATTT
    GATATGTATGGTTTTAAAGCGACATACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
    GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTC
    CATAGTCAACGTTGTGATTGTGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAA
    CATGGTGGCATGATTATTTATCTACCTCAAGAAGGTCGTGGCATAGGATTGTTAAACAAA
    TTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
    GGTTTTGATGAAGATTTACGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAAC
    ATCGAACATATCAATTTATTAAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATAT
    GGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAGAAACGGTACATAATCATGAT
    TATATGGAAACGAAAAAAATAAAAATGGGTCATTTAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
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    720
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    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001758
  • symbol: RibB
  • description: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • length: 393
  • theoretical pI: 4.88903
  • theoretical MW: 44192.1
  • GRAVY: -0.324427

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.99.12?  ExPASy
    3,4-dihydroxy-2-butanone-4-phosphate synthase D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD 3,4-dihydroxy-2-butanone-4-phosphate synthase (TIGR00506; EC 4.1.99.12; HMM-score: 247.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD GTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 227)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined DHBP_synthase; 3,4-dihydroxy-2-butanone 4-phosphate synthase (PF00926; HMM-score: 272.8)
    and 2 more
    GTP_cyclohydro2; GTP cyclohydrolase II (PF00925; HMM-score: 207.9)
    DUF4224; Domain of unknown function (DUF4224) (PF13986; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9627
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0369
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002287
    • TAT(Tat/SPI): 0.000212
    • LIPO(Sec/SPII): 0.000578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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