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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001754
  • pan locus tag?: SAUPAN004452000
  • symbol: JSNZ_001754
  • pan gene symbol?: —
  • synonym:
  • product: alpha/beta hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001754
  • symbol: JSNZ_001754
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1808366..1809193
  • length: 828
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTGGAAGTGGGAAGCTGAAAATGATGCGAAAGGCGTCGTTGTTATTGCTCATAATATA
    TTAGAACATACGGGAAGATATGCATATGTTATTACGATGTTAAGAAGAAATGGCTATCAT
    GTGATTATGGGAGATTTGCCTGGTCAAGGTCAAACTTCAAGAGCTAATAAAGGACAAATT
    GAAAACTTTCAAACGTATCACGAAAGTTTGCTAGATTGGTTAAAGATAGCTAATGAATAT
    AAAATTCCTACTTATGTATTAGGGGTAGGACTTGGCGGTTTGATTTTATTAAATTTACTT
    GAAAAAGTAGCATTGCCAATCGAGGGGATGATGTTAATTTCTCCTATGCTAGAACTTCAA
    AAGAATGGAAAAAATCGTAAAGATAAACTTGTTTCAAATATAGGTAAAATTTCAAAAGAC
    ACACGTTTTAATGTTGGTGTAGAACCAAAAGATTTAACACGTAATTTAGAAATTGTTGAA
    GAAACAGTTAACGATGGTTTAATGTTAAAAAAAGCAACGTATCATTGGTATAATACAATT
    AATGAGACGATGAAAGATACGATGGCTCATATTCATGACATTCAACCAATGCCAACGCTA
    TTAATGTATGGAACGAAGGATTTAATTGTTGATACTAGGGCAATTGATGAGTTTAAAGAA
    AAATATCAAACACCTGAATTATATTTTAAAGCTTGGCAAGGTTTTTACCACGAGGTTCAC
    AATGAGCCTGAACGCGATGAAGTAATGCGTTATATTCTGACTTTCTTAAATAACAGCGTC
    AATACAATGGGTTTTATTGTTGAAGATGACGAAATTGTAGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    828

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001754
  • symbol: JSNZ_001754
  • description: alpha/beta hydrolase
  • length: 275
  • theoretical pI: 5.60706
  • theoretical MW: 31807.4
  • GRAVY: -0.342909

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 32.4)
    and 3 more
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 20.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 20.3)
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 16.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 143.6)
    and 6 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 52.2)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 22.2)
    DUF1057; Alpha/beta hydrolase of unknown function (DUF1057) (PF06342; HMM-score: 21.4)
    TPR (CL0020) ComR_TPR; ComR tetratricopeptide (PF18710; HMM-score: 14.5)
    AB_hydrolase (CL0028) LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 13.3)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8248
    • Cytoplasmic Membrane Score: 0.1717
    • Cell wall & surface Score: 0.0008
    • Extracellular Score: 0.0027
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004227
    • TAT(Tat/SPI): 0.000733
    • LIPO(Sec/SPII): 0.000546
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMGDLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEKVALPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVEETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEFKEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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