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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001721
  • pan locus tag?: SAUPAN004397000
  • symbol: acsA
  • pan gene symbol?: acsA
  • synonym:
  • product: acetate--CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001721
  • symbol: acsA
  • product: acetate--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1765140..1766846
  • length: 1707
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAAGTCGAAGTTTATAAAGGAGCGCAAGGTAAACATAACCTTAAAGATTATGAAGAA
    ACATATAATACTTTTGATTGGAAAGACGTAGAACAAGCATTTTCTTGGAGTGAAACTGGA
    AAAATGAACATGGCATATGAATGTATAGATCGCCATGTAGATCAAGGATTAGGGGATAAA
    ATAGCGTTAAATTACAAAGATGAGCACAGAAAAGAATCGTATACTTATAAAGATATGCAA
    CGGTTATCTAATAAAGCAGCGAATGTTTTGTCTGAACATGCAGAAGTTGACAAAGGTGAC
    AGAGTATTTATATTTATGTCGCGTACACCTGAACTATATTTTGCGTTGTTAGGTGTTTTA
    AAAATTGGTGCAATTGTTGGGCCGTTATTTGAAGCATTTATGGAAAAGGCAGTTGCGGAT
    AGATTAGAGAACAGTGAAGCTAAAGTGTTAATTACTAATAAGGCATTGTTACCTCGAGTA
    CCTGTAGATAAATTACCGAACTTGAAAAAAATTGTTGTCGTAGATGAGGATGTAGAAGAC
    AATTACATAGACTTCATTAGTTTGATGGAAACTGCTAGCGATGAATTTGACATTGAATGG
    TTAAAGTCGGATGATGGTTTGATTTTACATTATACATCAGGTTCTACTGGGCAACCTAAA
    GGTGTATTGCATGTTCAACAAGCAATGTTAGTGCACTATATTTCTGGAAAATATGTATTA
    GATTTACAAGAAGATGATGTTTATTGGTGTACAGCAGATCCAGGTTGGGTTACAGGAACA
    TCTTATGGTATTTTTGCACCATGGTTAAATGGCGCTACAAATTGTATAGCTGGTGGTCGC
    TTTTCACCAGAACAGTGGTATAGTATGATTGAAGATTTTAAAGTGACGATTTGGTATACG
    GCACCAACAGCTTTAAGAATGTTAATGAGTGCTGGTGACGATATTGTTGAGAAATATGAC
    TTGTCATCGTTACGTTCGATTCTATCAGTAGGTGAGCCTTTAAATCCTGAAGTTATAAAA
    TGGGCGAAAAAAGTATACGGTTTAACGGTGTTAGATACTTGGTGGATGACAGAAACAGGT
    GGACATATGATTGTTAACTATCCAACGATGGACGTCAAGCTTGGCTCAATGGGCAAACCA
    TTACCTGGTATTCAAGCTGCAATTATCGATGATGCAGGTAATGAATTACCACCAAATCGA
    ATGGGCAACCTTGCTATAAAAAAAGGCTGGCCATCAATGATGTATCGTATCTGGAAGAAT
    CCAGAAAAATATAAATCATATTTTATTGGAGACTGGTATGTATCTGGTGATTCGGCATAT
    AAAGATGAAGATGGTTACTTCTGGTTCCAAGCACGTGTTGATGATGTAATTATGACAGCT
    GGTGAACGAGTTGGACCATTTGAGGTTGAGTCTAAATTGGTTGAACACGAAGCAGTTGCC
    GAAGCAGGAATTATTGGTAAACCAGATCCGGTTCGCGGTGAAATAATTAAGGCGTTTGTT
    GCACTGAGAAAAGGATATGAACCAACAGACGAATTAAAAGAAGAAATTCGTATATTTGTT
    AAAGAAGGTTTGTCGGCACATGCAGCACCACGTGAAATCGAATTTAAAGATAAATTACCT
    AAAACACGGTCAGGTAAAATTATGAGACGTGTATTAAAAGCTTGGGAATTAAATTTAGAT
    GCTGGGGATTTAAGTACAATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1707

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001721
  • symbol: AcsA
  • description: acetate--CoA ligase
  • length: 568
  • theoretical pI: 4.81296
  • theoretical MW: 64378.1
  • GRAVY: -0.327817

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.1?  ExPASy
    Acetate--CoA ligase ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
  • TIGRFAM:
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 539.8)
    and 12 more
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 303.2)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 284.5)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 196.3)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 187.9)
    amino acid adenylation domain (TIGR01733; HMM-score: 172.7)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 156.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 155.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 136.1)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 132)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 94.1)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 49.7)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 34.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 280.5)
    and 2 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 84.9)
    AMP-binding_C_3; AMP-binding enzyme C-terminal domain (PF23562; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7312
    • Cytoplasmic Membrane Score: 0.2549
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0138
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015848
    • TAT(Tat/SPI): 0.000645
    • LIPO(Sec/SPII): 0.00221
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNMAYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRVFIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRVPVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQPKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCIAGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPLNPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDAGNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQARVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEPTDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLSTME

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, CodY (repression) regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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