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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001598
  • pan locus tag?: SAUPAN004205000
  • symbol: pxpB
  • pan gene symbol?: —
  • synonym:
  • product: 5-oxoprolinase subunit PxpB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001598
  • symbol: pxpB
  • product: 5-oxoprolinase subunit PxpB
  • replicon: chromosome
  • strand: -
  • coordinates: 1633030..1633764
  • length: 735
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGGATGTAAGATTTATTAACGAGCAGACAATTATGATTTATTTTGAAAATAAAATATCC
    GAAGAGACATATCGTAATGTCACAGCAATGGTACGGTGGATAAGAGAAAAAGAATTATTA
    GAAATTCAAGATATTGTCCCTTCGTATCGAGCAGTTCTAATATATTTTGATGAACAAGCT
    ATAACATCTTCTAAATTAATTGAAAATTTAGAACTTAACAAATTTAATGAAAAAAATGTT
    CATGCTGTGAATCAAACCAAACGTATTATTAAAATGCCTGTTCAATATGGTGGTACATAT
    GGACCTGATATTGAAGAAGTAGCAAAGCATAATCGAATAACTGTTGAGCAAGTTATTGAA
    AAACATACAAGTAAACCTTATTTAATATATATGCTAGGATTTATGCCAGGATTTCCATAC
    TTAGGCGGACTTGATGAACAACTACATACACCTAGACGTAATCAGCCAAGGTTAAAAATT
    CATGCAGGTTCTGTAGGTATTGCGAATAATCAAACAGGACTATATCCATCAGATTCTCCT
    GGCGGATGGCAAATTATTGGACGCACACCGTTAAAAGTATTTAGCAGCGAGCGTGAACCA
    ATGTCAATGTACGAAGCAGGAGAATGGATACAATTTTATGCAATAGATGAACAAGAATTT
    ATACAAATCGAAAGAGATATATCAGATGGCAACTTTAATGTTGATGACTGGGTGGTGATT
    GAAAATGTCAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    735

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001598
  • symbol: PxpB
  • description: 5-oxoprolinase subunit PxpB
  • length: 244
  • theoretical pI: 4.81762
  • theoretical MW: 28382
  • GRAVY: -0.434426

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.2.9?  ExPASy
    5-oxoprolinase (ATP-hydrolyzing) ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate
  • TIGRFAM:
    Hypothetical proteins Conserved sensor histidine kinase inhibitor, KipI family (TIGR00370; HMM-score: 199.8)
    and 1 more
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 49.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Cyclophil-like (CL0475) CT_C_D; Carboxyltransferase domain, subdomain C and D (PF02682; HMM-score: 238.1)
    and 1 more
    no clan defined RepB_C; Replication protein RepB, C-terminal (PF21861; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9137
    • Cytoplasmic Membrane Score: 0.0852
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0007
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001019
    • TAT(Tat/SPI): 0.000059
    • LIPO(Sec/SPII): 0.000131
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKELLEIQDIVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTKRIIKMPVQYGGTYGPDIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKIHAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQEFIQIERDISDGNFNVDDWVVIENVN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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