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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001398
  • pan locus tag?: SAUPAN003833000
  • symbol: arlS
  • pan gene symbol?: arlS
  • synonym:
  • product: sensor histidine kinase ArlS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001398
  • symbol: arlS
  • product: sensor histidine kinase ArlS
  • replicon: chromosome
  • strand: -
  • coordinates: 1415134..1416489
  • length: 1356
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACAAAACGTAAATTGCGCAATAACTGGATTATTGTTACCACGATGATTACGTTTGTC
    ACGATATTTTTGTTTTGTTTAATTATTATTTTTTTCTTGAAAGATACACTGCATAATAGT
    GAGCTTGATGATGCAGAACGAAGCTCAAGCGATATTAATAATTTATTTCATTCTAAGCCT
    GTTAAAGATATATCTGCATTAGACTTGAATGCATCTTTAGGTAATTTTCAAGAGATTATT
    ATTTATGATGAGCATAATAATAAATTATTTGAGACATCGAATGATAACACAGTGAGAGTT
    GAACCTGGATATGAACACCGGTATTTTGACCGCGTAATAAAAAAACGCTATAAAGGCATT
    GAATATTTAATTATTAAAGAACCAATCACAACGCAAGATTTCAAAGGGTATAGCTTGTTA
    ATTCATTCACTAGAAAATTATGATAACATCGTAAAATCATTGTATATCATTGCGCTCGCA
    TTTGGAGTGATTGCAACAATTATAACTGCCACAATCAGTTATGTATTTTCAACTCAAATA
    ACTAAACCGCTTGTCAGTTTATCAAATAAAATGATTGAGATTCGACGAGATGGTTTTCAA
    AATAAATTGCAATTAAATACAAATTATGAAGAAATAGATAATTTAGCAAATACGTTTAAT
    GAGATGATGAGCCAAATTGAAGAATCATTTAATCAACAAAGACAATTTGTTGAAGATGCG
    TCACATGAATTACGAACACCATTACAAATTATTCAAGGTCATTTAAATTTGATTCAGCGA
    TGGGGAAAAAAAGACCCAGCAGTATTAGAAGAATCGTTAAATATTTCTATTGAAGAAATG
    AATCGTATCATAAAATTAGTCGAAGAATTACTTGAATTGACTAAAGGAGATGTAAATGAC
    ATTTCTTCTGAAGCGCAGACCGTGCATATTAATGATGAAATTCGCTCGCGAATACACTCA
    TTAAAACAATTGCATCCTGATTATCAATTTGATACGGATCTGACATCTAAAAATCTAGAA
    ATTAAAATGAAACCTCATCAATTCGAACAATTATTTTTAATCTTTATTGATAATGCAATT
    AAATATGATGTGAAGAATAAGAAAATTAAAGTTAAGACAAGGTTAAAAAATAAGCAAAAA
    ATAATTGAAATTACAGATCATGGAATTGGTATTCCAGAGGAAGATCAAGATTTCATTTTT
    GATCGCTTTTATCGAGTGGATAAATCTCGTTCAAGAAGTCAAGGCGGTAATGGACTCGGA
    TTATCTATTGCGCAAAAAATCATTCAATTAAACGGTGGATCGATTAAAATTAAAAGTGAA
    ATTAACAAAGGAACAACGTTTAAAATCATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001398
  • symbol: ArlS
  • description: sensor histidine kinase ArlS
  • length: 451
  • theoretical pI: 6.97701
  • theoretical MW: 52399.7
  • GRAVY: -0.33592

⊟Function[edit | edit source]

  • TIGRFAM:
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 197.2)
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 177.3)
    and 8 more
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 91)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 89)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 93.2)
    and 16 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 60)
    Cache (CL0165) ArlS_N; ArlS sensor domain (PF18719; HMM-score: 37.1)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.6)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 22.8)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 18.9)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 18.9)
    no clan defined DUF2207_C; Predicted membrane protein (DUF2207) C-terminal domain (PF20990; HMM-score: 15.9)
    TSPAN_4TM-like (CL0347) Tetraspanin; Tetraspanin family (PF00335; HMM-score: 15)
    no clan defined Carbpep_Y_N; Carboxypeptidase Y pro-peptide (PF05388; HMM-score: 14.2)
    ZapG-like; Z-ring associated protein G-like (PF06295; HMM-score: 13.9)
    MukF_C; MukF C-terminal domain (PF17193; HMM-score: 12.8)
    Clusterin; Clusterin (PF01093; HMM-score: 12)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 9.9)
    DUF2232; Predicted membrane protein (DUF2232) (PF09991; HMM-score: 9)
    DUF4133; Domain of unknown function (DUF4133) (PF13571; HMM-score: 8.9)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9986
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0012
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009038
    • TAT(Tat/SPI): 0.000301
    • LIPO(Sec/SPII): 0.004974
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDDAERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEPGYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALAFGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANTFNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNISIEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDLTSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIPEEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKIIF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Fur* (repression) regulon, SaeR (activation) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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