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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001314
  • pan locus tag?: SAUPAN003703000
  • symbol: JSNZ_001314
  • pan gene symbol?: —
  • synonym:
  • product: Cof-type HAD-IIB family hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001314
  • symbol: JSNZ_001314
  • product: Cof-type HAD-IIB family hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1322546..1323349
  • length: 804
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACAAATTATAAAGTTGTCGTTTTAGATATGGATGACACATTGCTAAATTCAGATAAT
    GTGATATCAGAAGAAACTGCAAATTATTTAACAGCAATTCAAGATGAAGGTTATTATGTT
    GTTTTAGCATCTGGTAGACCTACTGAAGGTATGATTCCAACTGCTAGAGATTTAAAATTA
    CCTGAACATCATAGCTATATTATTAGTTATAACGGTAGTAAAACGATTAACATGACTAAT
    GAAGAAGTAGAAGTAAGTAAATCGATTGGTAAGCAAGATTTCGATGAAATTGTAGATTAT
    TGTCGAGACAGAGGCTTTTTCGTTCTTACATATCATGATGGTCAAATTATTTACGACAGC
    GAACATGAGTATATGAATATTGAAGCAGAATTAACAGGTTTACCGATGAAACGTGTTGAT
    GATATCAAAGCGTATATTCAAGGCGATGTACCCAAGGTCATGGGTGTAGATTATGTAGCG
    AATATTACAGAAGCTAGAATTGATTTGAATGGTGTGTTCAATGATAATGTAGATGCTACG
    ACAAGTAAGCCATTCTTCTTAGAATTTATGGCCAAAGATGTTTCAAAAGGTAATGCAATT
    AAAGCGTTATGTCACAAATTGGGATATTCGGTGGATCAAGTCATTGCTTTTGGTGATAGT
    ATGAATGATAAATCAATGTTTGAAGTCGCAGGTCTAGCTATTGCTATGGGGAATGCATCA
    GATGAACTTAAGCAATATGCAGATGAAGTTACGTTGGACCATAATGAAAATGGTATTCCA
    CATGCGCTCAAAAAATTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001314
  • symbol: JSNZ_001314
  • description: Cof-type HAD-IIB family hydrolase
  • length: 267
  • theoretical pI: 4.29458
  • theoretical MW: 29822.4
  • GRAVY: -0.271536

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.3.-?  ExPASy
  • TIGRFAM:
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 186.2)
    and 24 more
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 90.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 84.9)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 84.4)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 50.1)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 35.1)
    Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 33.1)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 32.9)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 32.8)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 32.4)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 32.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 27.7)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 23.9)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 22.8)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 22.2)
    Metabolism Energy metabolism Anaerobic phenylphosphate carboxylase, delta subunit (TIGR02726; HMM-score: 21.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 17.7)
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 17.7)
    hemimethylated DNA binding domain (TIGR02097; HMM-score: 16.5)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16.1)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 14.6)
    Cellular processes Cellular processes Adaptations to atypical conditions trehalose-phosphatase (TIGR00685; EC 3.1.3.12; HMM-score: 12.7)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 12.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 12.4)
    Metabolism Energy metabolism ATP-proton motive force interconversion Na,H/K antiporter P-type ATPase, alpha subunit (TIGR01106; EC 3.6.3.-; HMM-score: 11.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 223.9)
    and 13 more
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 59)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 33.3)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 27.1)
    DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 20.1)
    Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 19.2)
    no clan defined DUF5769; Family of unknown function (DUF5769) (PF19073; HMM-score: 17)
    YccV-like; Hemimethylated DNA-binding protein YccV like (PF08755; HMM-score: 15.6)
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 15.3)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 13.1)
    no clan defined DUF6591; Family of unknown function (DUF6591) (PF20234; HMM-score: 12.9)
    HAD (CL0137) LNS2_PITM1-3; PITM 1-3, LNS2 domain (PF24694; HMM-score: 12.9)
    6PGD_C (CL0106) UDPG_MGDP_dh; UDP-glucose/GDP-mannose dehydrogenase family, central domain (PF00984; HMM-score: 12.6)
    TIM_barrel (CL0036) Glyco_hydro_97; Glycoside hydrolase 97 (PF10566; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9989
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.001
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005564
    • TAT(Tat/SPI): 0.000279
    • LIPO(Sec/SPII): 0.000792
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLASGRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCRDRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVANITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFGDSMNDKSMFEVAGLAIAMGNASDELKQYADEVTLDHNENGIPHALKKLL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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