PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001280
• pan locus tag^?: SAUPAN003626000
• symbol: glnA
• pan gene symbol^?: glnA
• synonym:
• product: type I glutamate--ammonia ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001280
• symbol: glnA
• product: type I glutamate--ammonia ligase
• replicon: chromosome
• strand: +
• coordinates: 1297414..1298754
• length: 1341
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
  AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
  GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
  GGTTTCGTACGTATCGAAGAATCTGACATGTATTTACATCCAGATTTAGATACTTGGGTA
  ATCTTCCCATGGACTGCTGGTCAAGGTAAAGTAGCACGTTTAATTTGTGATGTATATAAA
  ACAGATGGTACGCCATTTGAAGGGGATCCACGTGCAAACTTAAAACGTGTATTAAAAGAA
  ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCAGAATTCTTCTTGTTT
  AAGTTGGATGAAAAAGGAGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
  TTAGCACCTACAGATTTAGGAGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
  ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCACCGGGTCAACATGAAATCGAC
  TTTAAATATGCGGATGCAGTTACAGCATGTGATAACATCCAAACATTTAAATTGGTTGTT
  AGAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAGCCATTATTTGGT
  GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
  TTTGATCCGAATACTGAAATGGGCTTAACAGAAACAGCTTACCAATTCACAGCTGGTGTA
  CTTAAAAACGCACGTGGATTTACAGCGGTATGTAACCCATTAGTAAACTCATACAAACGT
  TTAGTTCCTGGTTATGAAGCACCATGTTATATTGCATGGAGCGGTAAAAACCGTTCACCG
  TTAATCCGTGTACCATCTTCAAGAGGATTATCAACTCGTATTGAAGTACGTTCAGTTGAC
  CCAGCTGCGAACCCATATATGGCGTTAGCTGCAATTTTAGAAGCTGGTTTAGACGGTATT
  AAAAATAAATTAAAAGTTCCAGAACCTGTTAACCAAAATATTTACGAAATGAATCGTGAA
  GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTACACAGCATTAAAAGCA
  ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAAACATATCTATAATCAATTTATT
  AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTATCTGAATGGGAAAGAGAT
  CAGTACATGAAGCAATATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1341
  

⊟Protein

⊟General

• locus tag: JSNZ_001280
• symbol: GlnA
• description: type I glutamate--ammonia ligase
• length: 446
• theoretical pI: 4.89483
• theoretical MW: 50882.4
• GRAVY: -0.440359

⊟Function

• reaction:
  EC 6.3.1.2?  ExPASy
  Glutamine synthetase ATP + L-glutamate + NH₃ = ADP + phosphate + L-glutamine
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 580.8)
  and 1 more
  glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 465.3)
  and 3 more
  no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 113.6)
  UBA (CL0214) UBA_NBR1_C; Next to BRCA1 gene 1 protein-like, C-terminal UBA domain (PF24932; HMM-score: 15.4)
  no clan defined Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9841
  • Cytoplasmic Membrane Score: 0.0007
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0151
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001979
  • TAT(Tat/SPI): 0.000395
  • LIPO(Sec/SPII): 0.000266
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVRTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGKHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications