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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001280
  • pan locus tag?: SAUPAN003626000
  • symbol: glnA
  • pan gene symbol?: glnA
  • synonym:
  • product: type I glutamate--ammonia ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001280
  • symbol: glnA
  • product: type I glutamate--ammonia ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1297414..1298754
  • length: 1341
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
    AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
    GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
    GGTTTCGTACGTATCGAAGAATCTGACATGTATTTACATCCAGATTTAGATACTTGGGTA
    ATCTTCCCATGGACTGCTGGTCAAGGTAAAGTAGCACGTTTAATTTGTGATGTATATAAA
    ACAGATGGTACGCCATTTGAAGGGGATCCACGTGCAAACTTAAAACGTGTATTAAAAGAA
    ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCAGAATTCTTCTTGTTT
    AAGTTGGATGAAAAAGGAGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
    TTAGCACCTACAGATTTAGGAGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
    ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCACCGGGTCAACATGAAATCGAC
    TTTAAATATGCGGATGCAGTTACAGCATGTGATAACATCCAAACATTTAAATTGGTTGTT
    AGAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAGCCATTATTTGGT
    GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
    TTTGATCCGAATACTGAAATGGGCTTAACAGAAACAGCTTACCAATTCACAGCTGGTGTA
    CTTAAAAACGCACGTGGATTTACAGCGGTATGTAACCCATTAGTAAACTCATACAAACGT
    TTAGTTCCTGGTTATGAAGCACCATGTTATATTGCATGGAGCGGTAAAAACCGTTCACCG
    TTAATCCGTGTACCATCTTCAAGAGGATTATCAACTCGTATTGAAGTACGTTCAGTTGAC
    CCAGCTGCGAACCCATATATGGCGTTAGCTGCAATTTTAGAAGCTGGTTTAGACGGTATT
    AAAAATAAATTAAAAGTTCCAGAACCTGTTAACCAAAATATTTACGAAATGAATCGTGAA
    GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTACACAGCATTAAAAGCA
    ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAAACATATCTATAATCAATTTATT
    AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTATCTGAATGGGAAAGAGAT
    CAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001280
  • symbol: GlnA
  • description: type I glutamate--ammonia ligase
  • length: 446
  • theoretical pI: 4.89483
  • theoretical MW: 50882.4
  • GRAVY: -0.440359

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamine synthetase ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 580.8)
    and 1 more
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 465.3)
    and 3 more
    no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 113.6)
    UBA (CL0214) UBA_NBR1_C; Next to BRCA1 gene 1 protein-like, C-terminal UBA domain (PF24932; HMM-score: 15.4)
    no clan defined Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9841
    • Cytoplasmic Membrane Score: 0.0007
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0151
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001979
    • TAT(Tat/SPI): 0.000395
    • LIPO(Sec/SPII): 0.000266
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVRTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGKHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GlnR* (repression) regulon, CodY (repression) regulon
    GlnR*(TF)important in Nitrogen assimilation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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