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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001274
pan locus tag^?: SAUPAN003618000
symbol: miaA
pan gene symbol^?: miaA
synonym:
product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001274
symbol: miaA
product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
replicon: chromosome
strand: +
coordinates: 1292295..1293230
length: 936
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAATAAAAATAAGCCTTTTATTGTAGTAATTGTGGGGCCAACTGCTTCAGGTAAAACA
GAGCTTAGCATAGAACTCGCGAAGCGTATCAATGGTGAAATCATAAGCGGTGATTCTATG
CAAGTCTACAAACATATGAATATTGGAACTGCAAAAGTAACACCTGAAGAAATGGATGGT
ATTCCACATCATTTAATTGATATCTTGAATCCTGATGATACATTTTCAGCATATGAATTC
AAGCGATTAGCAGAAGATTTAATTACTGATATAACGAATAGAGGTAAAGTTCCAATCATA
GCAGGTGGAACAGGCTTATATATTCAATCATTAATATATAATTATGAATTAGAAGATGAA
ACAGTTACACCTGCACAATTATCCATAGTTAAACAAAAGTTATCTGCATTAGAACATTTA
GATAATCAGCAACTACACGATTATTTAGCTCAATTTGATGCGGTTTCTGCAGAAAATATT
CACCCTAACAACCGCCAAAGAGTGTTGCGCGCTATTGAATATTATTTAAAAACAAAAAAA
CTTTTGAGTAATCGCAAGAAAGTGCAACAATTTACTGAAAATTATGATACATTATTATTA
GGGATTGAAATGTCGCGTAAAACATTATATTCAAGAATAAATAAACGTGTTGATATTATG
TTGGATCACGGATTATTTAGAGAAGTGCAACAACTTGTTGAACAAGGCTATGAATCTTGC
CAAAGTATGCAAGCTATTGGATATAAAGAATTAATACCTGTGATTAACGGACAAATGATT
TATGAAGATGCTGTCAATGATTTAAAGCAACATTCACGCCAATATGCAAAACGACAAATG
ACATGGTTCAAGAATAAAATGAGTGTTCATTGGTTAGATAAAGAAAATATGTCACTTCAA
ATGATGTTAGATGAGATTACAACCCAGATTAAGTAA
60
120
180
240
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420
480
540
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840
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936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001274
symbol: MiaA
description: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
length: 311
theoretical pI: 6.80558
theoretical MW: 35868
GRAVY: -0.431833

⊟Function[edit | edit source]

reaction:
EC 2.5.1.75? ExPASy
tRNA dimethylallyltransferase Dimethylallyl diphosphate + adenine³⁷ in tRNA = diphosphate + N⁶-dimethylallyladenine³⁷ in tRNA
TIGRFAM:
Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dimethylallyltransferase (TIGR00174; EC 2.5.1.75; HMM-score: 324.5)
and 4 more
carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 15.3)
Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 13.4)
putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 13.2)
Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 10.8)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
P-loop_NTPase (CL0023) IPPT; IPP transferase (PF01715; HMM-score: 294.1)
and 7 more
IPT; Isopentenyl transferase (PF01745; HMM-score: 34.6)
AAA_33; AAA domain (PF13671; HMM-score: 18.6)
AAA_18; AAA domain (PF13238; HMM-score: 15.9)
Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 15.8)
AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.8)
RNA_helicase; RNA helicase (PF00910; HMM-score: 13)
Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9967
- Cytoplasmic Membrane Score: 0.0018
- Cell wall & surface Score: 0.0006
- Extracellular Score: 0.0008
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.071303
- TAT(Tat/SPI): 0.001764
- LIPO(Sec/SPII): 0.036275
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENIHPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : JSNZ_001273 > miaA > hfq

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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