PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001103
• pan locus tag^?: SAUPAN003374000
• symbol: pheS
• pan gene symbol^?: pheS
• synonym:
• product: phenylalanine--tRNA ligase subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001103
• symbol: pheS
• product: phenylalanine--tRNA ligase subunit alpha
• replicon: chromosome
• strand: +
• coordinates: 1105968..1107026
• length: 1059
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGTCTGAACAACAAACAATGTCAGAGTTAAAACAACAAGCGCTTGTAGATATTAATGAA
  GCAAATGATGAACGTGCACTGCAAGAAGTTAAAGTGAAATACTTAGGTAAAAAAGGGTCA
  GTTAGCGGACTAATGAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGT
  CAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATG
  TTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCA
  GGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAA
  GACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACAAGATCAT
  TATAACTTCGAAATGCTGAATTTACCTAAATCACACCCTGCACGTGATATGCAAGATAGT
  TTCTATATTACGGATGAAATTTTATTACGTACGCATACATCACCAGTGCAGGCACGTACG
  ATGGAATCACGTCATGGTCAAGGTCCAGTTAAAATTATTTGCCCTGGTAAAGTGTATCGT
  CGTGACTCTGATGATGCGACACATAGTCATCAATTTACACAAATCGAAGGATTAGTTGTT
  GATAAAAACGTTAAAATGAGTGATTTGAAAGGTACTTTAGAATTGTTAGCTAAGAAATTA
  TTTGGTTCTGATCGTGAAATTCGTTTACGTCCAAGTTACTTCCCATTCACTGAACCTTCT
  GTAGAAGTTGATGTGTCATGTTTTAAATGTAAAGGAAAAGGTTGTAATGTGTGTAAACAC
  ACAGGATGGATTGAAATTTTAGGTGCTGGAATGGTACATCCTAATGTATTAGAAATGGCT
  GGTTTTGATTCTTCAGAGTACTCTGGATTTGCATTTGGTATGGGACCAGACCGTATTGCA
  ATGTTGAAATATGGTATAGAAGATATTCGTCATTTCTATACTAATGATGTGAGATTTTTA
  GATCAATTTAAAGCGGTAGAAGATAGAGGTGACATGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1059
  

⊟Protein

⊟General

• locus tag: JSNZ_001103
• symbol: PheS
• description: phenylalanine--tRNA ligase subunit alpha
• length: 352
• theoretical pI: 5.47515
• theoretical MW: 40122.4
• GRAVY: -0.547443

⊟Function

• reaction:
  EC 6.1.1.20?  ExPASy
  Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 380.2)
  and 7 more
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase (TIGR00469; EC 6.1.1.20; HMM-score: 124.3)
  Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 49.2)
  Metabolism Amino acid biosynthesis Serine family O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 49.2)
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00471; EC 6.1.1.20; HMM-score: 19.2)
  Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 15.6)
  Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 13.3)
  Genetic information processing Transcription Degradation of RNA ribonuclease, Rne/Rng family (TIGR00757; EC 3.1.4.-; HMM-score: 6.9)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 363.8)
  and 3 more
  tRNA_bind_arm (CL0298) Phe_tRNA-synt_N; Aminoacyl tRNA synthetase class II, N-terminal domain (PF02912; HMM-score: 83.7)
  tRNA_synt_II (CL0040) tRNA_synthFbeta; Phenylalanyl tRNA synthetase beta chain CLM domain (PF17759; HMM-score: 19.6)
  tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9976
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0024
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003802
  • TAT(Tat/SPI): 0.003535
  • LIPO(Sec/SPII): 0.000434
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGLMKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGRHIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDSFYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGLVVDKNVKMSDLKGTLELLAKKLFGSDREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCNVCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYTNDVRFLDQFKAVEDRGDM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, N315, Newman, USA300_FPR3757

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications