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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001093
  • pan locus tag?: SAUPAN003358000
  • symbol: isdB
  • pan gene symbol?: isdB
  • synonym:
  • product: heme uptake protein IsdB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001093
  • symbol: isdB
  • product: heme uptake protein IsdB
  • replicon: chromosome
  • strand: -
  • coordinates: 1096318..1098249
  • length: 1932
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACAAACAGCAAAAAGAATTTAAATCATTTTATTCAATTAGAAAGTCATCACTAGGC
    GTTGCATCTGTAGCAATTAGTACACTTTTATTATTAATGTCAAATGGCGAAGCACAAGCA
    GCTGAAGAAACAGGTGGTACAAATACAGAAGCACAACCAAAAACTGAAGCAGTTGCAAGT
    CCAACAACAACTGAAAAAGCTCCAGAAGCTAAACCAGTGGCTAATGCTGTCTCAGTATCT
    AATAAAGAAGTTGAGGCTCCTACTTCTGAAACAAAAGAAGCTAAAGAAGTTAAAGAAGTT
    AAAGCCCCTAAGGAAACAAAAGCAGTTAAACCAGCAGCAAAAGCCGATAACAATACATAT
    CCTATTTTGAATCAGGAACTTAGAGAAGCGATTAAAAACCCTGCAATAAAAGATAAAGAT
    CATAGCGCACCAAACTCTCGTCCAATTGATTTTGAAATGAAAAAAGAAAATGGTGAGCAA
    CAATTTTATCATTATGCCAGCTCTGTTAAACCTGCTAGAGTTATTTTCACTGATTCAAAA
    CCAGAAATTGAATTAGGATTACAATCAGGTCAATTTTGGAGAAAATTTGAAGTTTATGAA
    GGTGACAAAAAGTTGCCAATTAAATTAGTATCATACGATACTGTTAAAGATTACGCTTAC
    ATTCGCTTCTCTGTTTCAAATGGAACAAAAGCCGTTAAAATTGTAAGTTCAACTCACTTC
    AATAACAAAGAAGAAAAATACGATTACACATTAATGGAATTCGCACAACCAATTTATAAC
    AGTGCAGATAAATTCAAAACTGAAGAAGATTATAAAGCTGAAAAATTATTAGCGCCATAT
    AAAAAAGCGAAAACACTAGAAAGACAAGTTTATGAATTAAATAAAATTCAAGATAAACTT
    CCTGAAAAATTAAAGGCTGAGTACAAGAAGAAATTAGAGGATACAAAGAAAGCTTTAGAT
    GAGCAAGTGAAATCAGCTATTACTGAATTCCAAAATGTACAACCAACAAATGAAAAAATG
    ACTGATTTACAAGATACAAAATATGTTGTTTATGAAAGTGTTGAGAATAACGAATCTATG
    ATGGATACTTTTGTTAAACACCCTATTAAAACAGGTATGCTTAACGGCAAAAAATATATG
    GTCATGGAAACTACTAATGACGATTACTGGAAAGATTTCATGGTTGAAGGTCAACGTGTT
    AGAACTATAAGCAAAGATGCTAAAAATAATACTAGAACAATTATTTTCCCATATGTTGAA
    GGTAAAAATCTATATGATGCTATCGTTAAAGTTCACGTAAAAACGATTGATTATGATGGA
    CAATACCATGTCAGAATCGTTGATAAAGAAGCATTTACAAAAGCCAATACCGATAAATCT
    AACAAAAAAGAACAACAAGATAACTCAGCTAAGAAGGAAGCTACTCCAGCTACGCCTAGC
    AAACCAACACCATCACCTGTTGAAAAAGAATCACAAAAACAAGACAGCCAAAAAGATGAC
    AATAAACAATTACCAAGTGTTGAAAAAGAAAATGACGCATCTAGTGAGTCAGGTAAAGAC
    AAAACGCCTGCTACAAAACCAACTAAAGGTGAAGTAGAATCAAGTAGTACAACTCCAACT
    AAGGTAGTATCTACGACTCAAAATGTTGCAAAACCAACAACTGCTTCATCAAAAACAACA
    AAAGATGTTGTTCAAACTTCAGCAGGTTCTAGCGAAGCAAAAGATAGTGCTCCATTACAA
    AAAGCAAACATTAAAAACACAAATGATGGACACACTCAAAGCCAAAACAATAAAAATACA
    CAAGAAAATAAAGCAAAATCATTACCACAAACTGGTGAAGAATCAAATAAAGATATGACA
    TTACCATTAATGGCATTACTAGCTTTAAGTAGCATCGTTGCATTCGTATTACCTAGAAAA
    CGTAAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1932

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001093
  • symbol: IsdB
  • description: heme uptake protein IsdB
  • length: 643
  • theoretical pI: 9.50789
  • theoretical MW: 72000.4
  • GRAVY: -0.9479

⊟Function[edit | edit source]

  • TIGRFAM:
    heme uptake protein IsdB (TIGR03657; HMM-score: 1476)
    and 4 more
    haptoglobin-binding heme uptake protein HarA (TIGR03658; HMM-score: 525.6)
    heme uptake protein IsdC (TIGR03656; HMM-score: 41.8)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 40.3)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 18.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Isd_H_B_linker; Iron-regulated surface determinant protein H/B, linker domain (PF20861; HMM-score: 125.8)
    E-set (CL0159) NEAT; Iron Transport-associated domain (PF05031; HMM-score: 117.4)
    and 5 more
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 36.4)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 28.4)
    DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 12.3)
    CCDC-167; Coiled-coil domain-containing protein 167 (PF15188; HMM-score: 12.2)
    Proton_antipo_C; NADH-dehyrogenase subunit F, TMs, (complex I) C-terminus (PF01010; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.0613
    • Cell wall & surface Score: 0.8905
    • Extracellular Score: 0.0481
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 40 aa
    • SP(Sec/SPI): 0.976328
    • TAT(Tat/SPI): 0.020988
    • LIPO(Sec/SPII): 0.001279
    • Cleavage Site: CS pos: 40-41. AQA-AE. Pr: 0.9645
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAEETGGTNTEAQPKTEAVASPTTTEKAPEAKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKAVKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKNLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLALSSIVAFVLPRKRKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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