PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001058
• pan locus tag^?: SAUPAN003317000
• symbol: pdhA
• pan gene symbol^?: pdhA
• synonym:
• product: pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001058
• symbol: pdhA
• product: pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha
• replicon: chromosome
• strand: +
• coordinates: 1062498..1063610
• length: 1113
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  ATGGCTCCTAAGTTACAAGCCCAATTCGATGCAGTAAAAGTTTTAAATGATACTCAATCG
  AAATTTGAAATGGTTCAAATTTTGGACGAGAATGGTAACGTCGTAAATGAAGACTTAGTA
  CCTGATCTTACGGATGAACAATTAGTAGAATTAATGGAAAGAATGGTATGGACTCGTATC
  CTTGATCAACGTTCTATCTCATTAAACAGACAAGGACGTTTAGGTTTCTATGCACCAACT
  GCTGGTCAAGAAGCATCACAATTAGCGTCACAATACGCTTTAGAAAAAGAAGATTACATT
  TTACCGGGATACAGAGATGTTCCTCAAATTATTTGGCATGGTTTACCATTAACTGAAGCT
  TTCTTATTCTCAAGAGGTCACTTCAAAGGAAATCAATTCCCTGAAGGCGTTAATGCATTG
  AGCCCACAAATTATTATCGGTGCACAATACATTCAAGCTGCTGGTGTTGCATTTGCACTT
  AAAAAACGTGGTAAAAATGCAGTTGCAATCACTTACACTGGTGACGGTGGTTCTTCACAA
  GGTGATTTCTACGAAGGTATTAACTTTGCAGCAGCTTATAAAGCACCTGCAATTTTCGTT
  ATTCAAAACAATAACTATGCAATTTCAACACCAAGAAGCAAGCAAACTGCTGCTGAAACA
  TTAGCTCAAAAAGCAATTGCTGTAGGTATTCCTGGTATCCAAGTTGATGGTATGGATGCG
  TTAGCTGTATATCAAGCAACTAAAGAAGCACGTGACCGCGCAGTTGCAGGTGAAGGTCCA
  ACATTAATTGAAACTATGACATATCGTTATGGTCCTCATACAATGGCTGGTGACGATCCA
  ACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGT
  TTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATT
  GAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAA
  ACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCAAAACTTAGCAGAACAA
  TATGAAATTTACAAAGAGAAGGAGTCGAAGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1113
  

⊟Protein

⊟General

• locus tag: JSNZ_001058
• symbol: PdhA
• description: pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha
• length: 370
• theoretical pI: 4.62739
• theoretical MW: 41382.3
• GRAVY: -0.484054

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 488)
  and 5 more
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 217.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
  Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 16.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 352.9)
  and 3 more
  DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.3)
  TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 16.8)
  no clan defined RNase_J_C; Ribonuclease J C-terminal domain (PF17770; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8799
  • Cytoplasmic Membrane Score: 0.0412
  • Cell wall & surface Score: 0.0014
  • Extracellular Score: 0.0775
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000996
  • TAT(Tat/SPI): 0.00008
  • LIPO(Sec/SPII): 0.000142
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLTDEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILPGYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFALKKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAAETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMAGDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEADNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications