From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001013
  • pan locus tag?: SAUPAN003254000
  • symbol: JSNZ_001013
  • pan gene symbol?: patA
  • synonym:
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001013
  • symbol: JSNZ_001013
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • replicon: chromosome
  • strand: -
  • coordinates: 1014934..1016088
  • length: 1155
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAACTTTCTTTAAATTCTAATTCTAAATATTTAAGAGCACCAAGTATTCGCCAATTT
    TCAAATCGTATGAATAATTTGGATGATTGTGTTAATTTAACTATTGGCCAACCAGACTTC
    CCGATGCCTGATGTTGTGAAAAAGGCATATATTGATGCTATTAACAATGACAAGACATCA
    TACTCTCACAATAAAGGGTTATTAGAAACTCGCGAAGCAATTAGTCAATACTTCAAAAAT
    CGTTATCATTTTTCCTATGATCCTGAAGAAATTATTGTGACAAATGGAGCAAGTGAAGCA
    ATAGACACAACGTTACGTTCTATCATAGAGCCTGGAGATGAAATTATAATACCGGGACCA
    ATTTATGCAGGCTACATACCACTCATCGAAGTACTAGGTGGTAAACCAATTTATATTGAT
    ACAACAGCAACACAATTTAAAATTACACCTGATGCATTAGAAAGTCATATTTCTCCAAAG
    ACAAAAGCTGTCTTGTTAAATTATCCGACTAATCCAACTGGCGTAGTTTTAAAAAGAAAT
    GAAGTTTTAAATATCGTAAATGTATTAAAAAAATATCCGTTATTTATTATTAGCGATGAG
    ATTTATGCTGAAAATACATTTAGTGGTAAACATGTATCCTTCGCTGAATTTGAAGACATT
    CGTGATCAATTAATATTAATTGGTGGTTTAAGTAAATCACACTCAGCAACCGGAATAAGA
    ATTGGTTTTCTATTAGGACCTCAATATTTGATTGATAAATTAACATTTATGCACGCCTAT
    AATTGTATTTGTGCCAATGTTCCAGCTCAAATAGCATGTATAACAGCACTTAACGAAGGA
    TTAGAAGCTCCTAAATATATGAACGAAGCTTATGTAGAACGAAGAAATTATTTAGTATCT
    GAATTAACTAAATTAGGTTTTGAGATTACCGCTCAACCTGAAGGCGCGTTTTATATTTTC
    CCAAGTATTAAACATATCACCGATGACGATTTCGAATTTTGTATCGATTTACTTGAATCA
    ACACATTTAGCAATCGTTCCAGGCTCATCCTTTACAGAATTCGGTAAAGGATTTGTAAGG
    ATTTCCTATGCTTATGAAATGGATGTTTTAAAAGAAGGTATGAAGAGACTTGCTAAATAT
    TTAAATACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001013
  • symbol: JSNZ_001013
  • description: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • length: 384
  • theoretical pI: 5.66622
  • theoretical MW: 43160.2
  • GRAVY: -0.115365

⊟Function[edit | edit source]

  • TIGRFAM:
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 209.8)
    and 28 more
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 156.6)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 138.5)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 135.8)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 129.3)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 126.5)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 122.3)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 122.3)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 119.2)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 101.1)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 98.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 85)
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 53.6)
    Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 40.6)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides TDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 28)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 27.9)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 24.9)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 24.4)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 22.9)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 17.4)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 17.4)
    aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 16.1)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 15.5)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 13)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 12.9)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 12.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 12.5)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 12.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 221.8)
    and 4 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 39.8)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 34.1)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 32.9)
    OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 18.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9931
    • Cytoplasmic Membrane Score: 0.0014
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0053
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010565
    • TAT(Tat/SPI): 0.000464
    • LIPO(Sec/SPII): 0.001211
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPDVVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAIDTTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPKTKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFEDIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITALNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFCIDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001013&oldid=106654"