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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000908
  • pan locus tag?: SAUPAN003046000
  • symbol: JSNZ_000908
  • pan gene symbol?: —
  • synonym:
  • product: FAD/NAD(P)-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000908
  • symbol: JSNZ_000908
  • product: FAD/NAD(P)-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 904836..905990
  • length: 1155
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCATTGGACAATTATCGGCGGTGGCATACAGGGAACTGCAATCGCACAAAAACTATTA
    TCAAGCGGATTAACAACAGACCGATTAACAATCATTGACCCACACGAAACTTTTTGCCAA
    AGGTTTAACTCATATACAAATCGAATAGAAATGCCTTATTTAAGATCACCGATTGTACAT
    CACGTACATCCACAACCGTTCCATCTAAAACAATTCGCTAAACAGCACCAATATACAAAT
    GCTTTTTATGGTCCTTATCAACGACCTGAATTGACAATGTTTATGGATCATATTGCACAT
    GCTTCTAAACAATATCAATTAGAGGATTGCTTGGTTCAAGGTTTAGTTCAAACTTTAGAT
    AAACAAGAAGACAAATGGCATATCAAGTTAGAAGATGGACAAATTATCACTACAGATTGC
    GTCGTTATTGCAATAGGCAGTACAAATATTCCGTTTATGCCTGACATTTTAAAAGACAAA
    CAGAATGTAAATCATATCTTCGAGAAAGAACTTGATCAAGTAGTATATGATAAGACCGAT
    CATATCGTTGGTAGCGGCATTACTGCTGCACATCTTGCACTTAAATTGTTAAATCATGAT
    AACGATAAAAAGATTCATTTATGGCTAAATAAAGATATTGAAATACATGACTTTGATGCT
    GATCCTGGTTGGTTAGGTCCGAAAAATATGTCTTCATTTTTAAGTACTAAAAGCATGCCT
    GAAAGAAATGCCATTGTACAACGCGAACGTCATAAAGGATCAATGCCTCACGAACTGTAC
    TTACGCCTTAAAAAACATATTAAAAATGGTCGTATAAATGTGCATAAAACACCTATCACT
    CAAATTAGTGGTGGTGTAATTAACACTGAAAATGATTCTGTTCCATATCAACAGATTATG
    GTTGCAACTGGTTTTGAACAAGATTTTATGTCACAACCACTTATTAAGCAATTAATACAA
    AATTATGATGCACCTATCAACGAATGTAATTACCCTGTTATTTCCGAAAAATTAGAATGG
    ATACCAAATCTATTTGTCGCAGGATGCTTTGCAGACTTAGAATTAGGACCATTTGGTAGA
    AATGTTATGGGTGGCCGTAAAGCTGCCGAACGCATTGAACAAGCATTTCTAAAACTACAA
    CAATATAGCGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000908
  • symbol: JSNZ_000908
  • description: FAD/NAD(P)-binding protein
  • length: 384
  • theoretical pI: 7.07664
  • theoretical MW: 43955
  • GRAVY: -0.403906

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 30.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 25.3)
    and 9 more
    lycopene cyclase family protein (TIGR01790; HMM-score: 21.5)
    putative histamine N-monooxygenase (TIGR04439; EC 1.14.13.-; HMM-score: 16.3)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 13.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 13.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 12.3)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 11.7)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 45.6)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 40.4)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 40)
    and 9 more
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25.3)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 23)
    no clan defined MptE-like; 6-hydroxymethylpterin diphosphokinase MptE-like (PF01973; HMM-score: 21.2)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 18.5)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17.5)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.6)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 13.4)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 12.8)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7528
    • Cytoplasmic Membrane Score: 0.2289
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0181
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02305
    • TAT(Tat/SPI): 0.002147
    • LIPO(Sec/SPII): 0.015907
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNSYTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHASKQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDKQNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDFDADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHKTPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVISEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Zur* (repression) regulon, GraR (repression) regulon
    Zur*(TF)important in Zinc homeostasis;  transcription unit predicted or transferred from N315 and NCTC8325 
    GraR(response regulator)important in cationic antimicrobial peptide (CAMP) resistance;  transcription unit predicted or transferred from N315 and NCTC8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Mélanie Falord, Ulrike Mäder, Aurélia Hiron, Michel Débarbouillé, Tarek Msadek
    Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways.
    PLoS One: 2011, 6(7);e21323
    [PubMed:21765893] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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