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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000726
  • pan locus tag?: SAUPAN002664000
  • symbol: prfB
  • pan gene symbol?: prfB
  • synonym:
  • product: peptide chain release factor 2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000726
  • symbol: prfB
  • product: peptide chain release factor 2
  • replicon: chromosome
  • strand: +
  • coordinates: join(761551..761622,761624..762661)
  • length: 1110
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAATTATCAGAAATCAAACGAAATATAGATAAGTATAATCAAGATTTAACACAAATT
    AGGGGGTCTCTTGACTTAGAGAACAAAGAAACTAATATTCAAGAATATGAAGAAATGATG
    GCAGAACCTAATTTTTGGGATAACCAAACGAAAGCGCAAGATATTATAGATAAAAATAAT
    GCGTTAAAAGCAATAGTTAATGGTTATAAAACACTACAAGCAGAAGTAGATGACATGGAT
    GCTACTTGGGATTTATTACAAGAAGAATTTGATGAAGAAATGAAAGAAGACTTAGAGCAA
    GAGGTCATTAATTTTAAGGCTAAAGTGGATGAATACGAATTGCAATTATTATTAGATGGG
    CCTCACGATGCCAATAACGCAATTCTAGAGTTACATCCTGGTGCAGGTGGCACGGAGTCT
    CAAGATTGGGCTAATATGCTATTTAGAATGTATCAACGTTATTGTGAGAAGAAAGGCTTT
    AAAGTTGAAACTGTTGATTATCTACCTGGGGATGAAGCAGGGATTAAAAGTGTAACATTG
    CTCATCAAAGGGCATAATGCTTATGGTTATTTAAAAGCTGAAAAAGGTGTACACCGACTA
    GTACGAATTTCTCCATTTGATTCATCAGGACGTCGTCATACATCATTTGCATCATGCGAC
    GTTATTCCAGATTTTAATAATGATGAAATAGAGATTGAAATCAATCCGGATGATATTACA
    GTTGATACATTCAGAGCTTCTGGTGCAGGTGGTCAGCATATTAACAAAACTGAATCGGCA
    ATACGAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAA
    ATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTG
    GAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGA
    AGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAAC
    GAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACATTGGACCATTTATCGAATCA
    TATTTAAGACAGACAATGTCGCACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1110

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000726
  • symbol: PrfB
  • description: peptide chain release factor 2
  • length: 369
  • theoretical pI: 4.57447
  • theoretical MW: 42331
  • GRAVY: -0.759079

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 2 (TIGR00020; HMM-score: 532.1)
    and 5 more
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 1 (TIGR00019; HMM-score: 305)
    Genetic information processing Protein synthesis Translation factors putative peptide chain release factor H (TIGR03072; HMM-score: 120.2)
    SH3 domain protein (TIGR04211; HMM-score: 9)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 4.7)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 4.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman
  • PFAM:
    no clan defined PCRF; PCRF domain (PF03462; HMM-score: 216.8)
    and 2 more
    RF (CL0337) RF-1; RF-1 domain (PF00472; HMM-score: 152.8)
    no clan defined Occludin_ELL; Occludin homology domain (PF07303; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9894
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0097
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005866
    • TAT(Tat/SPI): 0.00034
    • LIPO(Sec/SPII): 0.000817
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEVINFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFASCDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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