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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000510
  • pan locus tag?: SAUPAN002324000
  • symbol: JSNZ_000510
  • pan gene symbol?: araB
  • synonym:
  • product: ribulokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000510
  • symbol: JSNZ_000510
  • product: ribulokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 544133..545770
  • length: 1638
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTATAGCATTGGAATTGATTATGGAACAGCTTCAGGCCGTGTGTTTTTAATTAAT
    ACAACTAACGGTCAAGTAGTATCAAAATTTGTGAAACCATATACACATGGTGTCATTGAG
    AGTGAATTAAATGGTTTGAAAATACCACATACATATGCACTTCAAAATAGTAATGATTAT
    TTAGAAATTATGGAAGAAGGAATATCATATATAGTACGTGAATCAAAAATAGATCCAGAC
    AATATAGTAGGTATTGGTATAGACTTTACTTCATCTACTATTATTTTTACTGACGAAAAT
    CTTAACCCGGTACATAATTTAAAACAATTTAAAAACAATCCACATGCGTATGTGAAACTT
    TGGAAACATCATGGTGCATATAAAGAGGCAGAGAAATTATATCAAACTGCTATTGAAAAT
    AATAATAAGTGGTTAGGCCATTATGGATATAATGTTAGTAGTGAATGGATGATTCCCAAA
    ATAATAGAGGTCATGAATCGAGCACCAGAAATTATGGAAAAAACGGCTTATATTATGGAA
    GCGGGCGATTGGATTGTAAATAAATTAACTAATAAAAATGTACGCTCGAATTGTGGATTA
    GGTTTCAAAGCATTTTGGGAAGAAGAAACAGGGTTTCATTATGATTTATTTGATAAAATA
    GACCCCAAATTATCAAAAGTAATTCAAGATAAAGTATCTGCACCGGTTGTTAATATTGGT
    GAAGTAGTAGGGAAACTGGACGATAAAATGGCACAGAAATTAGGATTATCAAAAGAGACT
    ATGGTAAGTCCTTTTATTATTGATGCCCATGCTAGTTTATTAGGTATTGGGTCTGAAAAA
    GATAAAGAAATGACTATGGTGATGGGAACAAGCACATGCCATCTTATGTTAAATGAAAAG
    CAACATCAAGTGCCAGGTATATCAGGTTCTGTAAAAGGAGCAATTATTCCAGAATTATTT
    GCTTATGAAGCGGGGCAATCAGCAGTAGGTGATTTGTTTGAGTATGTCGCTAAGCAAGCA
    CCAAAGTCATATGTAGATGAAGCAGCAAATAGAAATATGACTGTATTTGAATTAATGAAT
    GAAAAGATAAAACATCAAATGCCAGGTGAAAGTGGGCTCATTGCTCTTGATTGGCATAAT
    GGAAATCGAAGTGTATTAAGTGATAGCAATTTAACAGGTTGTATCTTTGGATTAACTTTA
    CAAACTAAGCATGAGGATATTTATAGAGCATATTTAGAAGCTACAGCATTTGGTACTAAG
    ATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGC
    GGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAA
    CTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTC
    AGTGGTGGCGCACATAATTCAATTAATGATGCAGTTGATGCTATGAAAGAGCCAATTTTA
    TACGAAATTAATCCAGAAGCGGAAAAAGTACAAAGGTATGAAACATTATTTAAAGCTTAT
    AAGGCTTTACATGATATCCATGGTTATAAAAAAGCTAATATAATGAAAGATATCCAGAGT
    TTAAGAGTTGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000510
  • symbol: JSNZ_000510
  • description: ribulokinase
  • length: 545
  • theoretical pI: 6.14334
  • theoretical MW: 60962.4
  • GRAVY: -0.259266

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.16?  ExPASy
    Ribulokinase ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 553.4)
    and 5 more
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 208.1)
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 154.6)
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 89.4)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 68.1)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 59.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Actin_ATPase (CL0108) FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 127.9)
    and 4 more
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 56.7)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 15.9)
    no clan defined DUF4846; Domain of unknown function (4846) (PF16138; HMM-score: 15.8)
    TPR (CL0020) TPR_NPHP3; Nephrocystin-3 TPR domain (PF24885; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.64
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7087
    • Cytoplasmic Membrane Score: 0.009
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.2822
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013869
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.004046
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELNGLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIIEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEVVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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