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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000453
  • pan locus tag?: SAUPAN002255000
  • symbol: cysK
  • pan gene symbol?: cysK
  • synonym:
  • product: cysteine synthase A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000453
  • symbol: cysK
  • product: cysteine synthase A
  • replicon: chromosome
  • strand: +
  • coordinates: 486236..487168
  • length: 933
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCACAAAAACCAGTAGATAATATTACTCAAATTATTGGCGGTACACCGGTAGTCAAA
    TTGAGAAATGTAGTAGATGACAATGCAGCAGATGTTTATGTAAAATTGGAATATCAAAAT
    CCAGGTGGTTCTGTAAAGGATAGAATTGCTTTAGCAATGATTGAAAAAGCAGAGCGAGAA
    GGCAAAATTAAACCTGGCGATACAATTGTAGAACCAACAAGTGGTAATACAGGTATCGGT
    TTAGCATTTGTATGTGCTGCTAAAGGATATAAAGCAGTATTTACTATGCCCGAAACAATG
    AGCCAAGAGCGTCGTAATTTATTAAAAGCATACGGTGCGGAATTAGTTTTAACGCCTGGA
    TCAGAAGCGATGAAAGGTGCAATTAAAAAAGCTAAAGAATTGAAAGAAGAACATGGTTAC
    TTCGAGCCACAACAATTTGAAAACCCTGCGAACCCTGAAGTTCATGAGTTAACTACAGGT
    CCTGAGTTATTACAACAATTTGAAGGGAAAACTATCGATGCGTTCCTAGCTGGTGTTGGT
    ACTGGTGGTACGTTATCTGGTGTAGGTAAAGTTCTGAAAAAAGAATATCCTAACATCGAA
    ATTGTTGCTATAGAGCCTGAGGCTTCTCCAGTATTGAGCGGTGGTGAGCCAGGTCCACAT
    AAATTACAAGGTTTAGGTGCTGGATTTATTCCAGGCACTTTGAATACAGAAATCTATGAC
    AGTATTATTAAAGTAGGAAATGATACAGCGATGGAAATGTCTCGTCGAGTTGCTAAAGAG
    GAAGGTATTTTAGCAGGTATTTCATCAGGTGCTGCGATTTATGCTGCCATTCAAAAAGCA
    AAAGAATTAGGAAAAGGTAAAACAGTAGTAACAGTATTGCCGAGTAATGGTGAACGCTAC
    TTATCAACACCTTTATATTCATTCGATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000453
  • symbol: CysK
  • description: cysteine synthase A
  • length: 310
  • theoretical pI: 5.12885
  • theoretical MW: 32975.4
  • GRAVY: -0.237419

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.47?  ExPASy
    Cysteine synthase O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 463.6)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 440.6)
    and 12 more
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 326.6)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 293)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 245.2)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 73.8)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 73.7)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 72.7)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 48)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 41.3)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 33.4)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 33.4)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 23.2)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 23.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 270.5)
    and 2 more
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 19.6)
    Thiolase (CL0046) ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.992
    • Cytoplasmic Membrane Score: 0.0016
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0062
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003491
    • TAT(Tat/SPI): 0.000228
    • LIPO(Sec/SPII): 0.000529
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGSVKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTGPELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPGPHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAAIQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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