PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000396
• pan locus tag^?: SAUPAN002178000
• symbol: gmpC
• pan gene symbol^?: metQ2
• synonym:
• product: dipeptide ABC transporter glycylmethionine-binding lipoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000396
• symbol: gmpC
• product: dipeptide ABC transporter glycylmethionine-binding lipoprotein
• replicon: chromosome
• strand: +
• coordinates: 425569..426411
• length: 843
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGAAAAGATTGATTGGGTTAGTTATCGTAGCACTTGTATTATTAGCAGCGTGTGGTAGT
  AACAATGATAAAAAAGTAACAATTGGTGTCGCATCAAATGACACTAAGGCTTGGGAGAAG
  GTTAAAGAATTAGCTAAAAAAGATGATATTGATGTGGAGATTAAGCACTTTTCCGATTAC
  AATTTACCGAATAAAGCATTAAACGATGGTGATATTGATATGAATGCATTCCAACATTTT
  GCATTTTTAGATCAGTATAAGAAGGCACATAAAGGAACAAAGATTTCAGCATTGAGTACA
  ACAGTTTTAGCACCGTTGGGCATTTACTCAGATAAAATTAAAGATGTCCAAAAGGTTAAA
  GATGGTGCTAAAGTTGTCATTCCAAATGATGTATCAAACCAAGCACGTGCACTTAAACTA
  TTAGAAGCAGCTGGTTTAATAAAACTGAAAAAAGATTTCGGATTAGCAGGTACAGTGAAA
  GATATAACGTCAAATCCAAAACATTTAAAAATTACTGCAGTAGATGCACAGCAAACTGCG
  CGTGCTTTATCTGATGTCGATATTGCAGTTATTAATAACGGTGTAGCAACTAAAGCGGGG
  AAAGACCCTAAAAATGATCCGATATTTTTAGAAAAATCAAATTCAGATGCTGTAAAGCCA
  TATATTAATATTGTTGCAGTTAATGACAAAGACTTGGATAACAAAACATATGCTAAAATC
  GTAGAATTGTATCATTCAAAAGAAGCTCAAAAAGCGTTGCAGGAAGATGTCAAAGATGGA
  GAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAA
  TAA
  60
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  843
  

⊟Protein

⊟General

• locus tag: JSNZ_000396
• symbol: GmpC
• description: dipeptide ABC transporter glycylmethionine-binding lipoprotein
• length: 280
• theoretical pI: 9.50621
• theoretical MW: 30485.9
• GRAVY: -0.315714

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 197.7)
  and 1 more
  Cell structure Cell envelope Surface structures pilus (Caulobacter type) biogenesis lipoprotein CpaD (TIGR02522; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PBP (CL0177) Lipoprotein_9; NlpA lipoprotein (PF03180; HMM-score: 287.3)
  and 7 more
  NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 15.4)
  Flavoprotein (CL0042) FMN_red; NADPH-dependent FMN reductase (PF03358; HMM-score: 14.5)
  PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 12.7)
  DPBB (CL0199) IML1_N; IML1 N-terminal double psi beta barrel domain (PF23013; HMM-score: 12.7)
  NADP_Rossmann (CL0063) PylC-like_N; PylC-like, N-terminal domain (PF21360; HMM-score: 12.1)
  NTP_transf (CL0260) Polbeta; Polymerase beta, Nucleotidyltransferase (PF18765; HMM-score: 11.9)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9114
  • Cell wall & surface Score: 0.0043
  • Extracellular Score: 0.0843
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000461
  • TAT(Tat/SPI): 0.000068
  • LIPO(Sec/SPII): 0.999288
  • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKELAKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTVLAPLGIYSDKIKDVQKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVKDITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAVKPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIETSLAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.
Nucleic Acids Res: 2004, 32(11);3340-53
[PubMed:15215334] [WorldCat.org] [DOI] (I e)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)