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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000396
  • pan locus tag?: SAUPAN002178000
  • symbol: gmpC
  • pan gene symbol?: metQ2
  • synonym:
  • product: dipeptide ABC transporter glycylmethionine-binding lipoprotein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000396
  • symbol: gmpC
  • product: dipeptide ABC transporter glycylmethionine-binding lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 425569..426411
  • length: 843
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAGATTGATTGGGTTAGTTATCGTAGCACTTGTATTATTAGCAGCGTGTGGTAGT
    AACAATGATAAAAAAGTAACAATTGGTGTCGCATCAAATGACACTAAGGCTTGGGAGAAG
    GTTAAAGAATTAGCTAAAAAAGATGATATTGATGTGGAGATTAAGCACTTTTCCGATTAC
    AATTTACCGAATAAAGCATTAAACGATGGTGATATTGATATGAATGCATTCCAACATTTT
    GCATTTTTAGATCAGTATAAGAAGGCACATAAAGGAACAAAGATTTCAGCATTGAGTACA
    ACAGTTTTAGCACCGTTGGGCATTTACTCAGATAAAATTAAAGATGTCCAAAAGGTTAAA
    GATGGTGCTAAAGTTGTCATTCCAAATGATGTATCAAACCAAGCACGTGCACTTAAACTA
    TTAGAAGCAGCTGGTTTAATAAAACTGAAAAAAGATTTCGGATTAGCAGGTACAGTGAAA
    GATATAACGTCAAATCCAAAACATTTAAAAATTACTGCAGTAGATGCACAGCAAACTGCG
    CGTGCTTTATCTGATGTCGATATTGCAGTTATTAATAACGGTGTAGCAACTAAAGCGGGG
    AAAGACCCTAAAAATGATCCGATATTTTTAGAAAAATCAAATTCAGATGCTGTAAAGCCA
    TATATTAATATTGTTGCAGTTAATGACAAAGACTTGGATAACAAAACATATGCTAAAATC
    GTAGAATTGTATCATTCAAAAGAAGCTCAAAAAGCGTTGCAGGAAGATGTCAAAGATGGA
    GAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000396
  • symbol: GmpC
  • description: dipeptide ABC transporter glycylmethionine-binding lipoprotein
  • length: 280
  • theoretical pI: 9.50621
  • theoretical MW: 30485.9
  • GRAVY: -0.315714

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 197.7)
    and 1 more
    Cell structure Cell envelope Surface structures pilus (Caulobacter type) biogenesis lipoprotein CpaD (TIGR02522; HMM-score: 12.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PBP (CL0177) Lipoprotein_9; NlpA lipoprotein (PF03180; HMM-score: 287.3)
    and 7 more
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 15.4)
    Flavoprotein (CL0042) FMN_red; NADPH-dependent FMN reductase (PF03358; HMM-score: 14.5)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 12.7)
    DPBB (CL0199) IML1_N; IML1 N-terminal double psi beta barrel domain (PF23013; HMM-score: 12.7)
    NADP_Rossmann (CL0063) PylC-like_N; PylC-like, N-terminal domain (PF21360; HMM-score: 12.1)
    NTP_transf (CL0260) Polbeta; Polymerase beta, Nucleotidyltransferase (PF18765; HMM-score: 11.9)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 9.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9114
    • Cell wall & surface Score: 0.0043
    • Extracellular Score: 0.0843
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000461
    • TAT(Tat/SPI): 0.000068
    • LIPO(Sec/SPII): 0.999288
    • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKELAKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTVLAPLGIYSDKIKDVQKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVKDITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAVKPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIETSLAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.
Nucleic Acids Res: 2004, 32(11);3340-53
[PubMed:15215334] [WorldCat.org] [DOI] (I e)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)

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