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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000395
  • pan locus tag?: SAUPAN002177000
  • symbol: JSNZ_000395
  • pan gene symbol?: metP2
  • synonym:
  • product: methionine ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000395
  • symbol: JSNZ_000395
  • product: methionine ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 424873..425532
  • length: 660
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTGGTTCTGATTTAGACAGTGCACAGTTATTACAAGCATTGTACGAAACGCTATAT
    ATGGTATCTATTGCTTTATTTTTAGGAGCAGTGATTGGTATTCCATTAGGTGTCTTATTG
    GTAATTACTCGAAAACAAGGCATATGGCCGAATATAGTGATACATCAAGTTTTAAATCCT
    ATAATCAATATTTTAAGGTCACTACCATTTATTATTTTGTTAATTGCGATTGTACCATTC
    ACAAAATTAGTAGTAGGTACTTCAATTGGTACGACTGCTGCCATCGTGCCTTTAACAGTA
    TATGTGGCACCTTACATTGCAAGACTTGTTGAAAACTCATTATTAGAAGTAGACGAGGGG
    ATTATTGAAGCGGCGAAAGCGATGGGCGCTTCACCACTACAAATCATTAGATATTTTTTA
    ATTCCTGAAGCGTTAGGTTCATTAGTATTAGCAATTACCACTGCTATTATTGGACTTATT
    GGAAGTACGGCGATGGCAGGAGCTGTTGGCGGTGGTGGTATAGGAGACTTAGCTTTAGTG
    TATGGTTATCAAAGATTTGATACGACGGTCATTATTATTACCGTTATTGTATTAGTCATT
    ATTGTCCAAGTGATTCAAACGCTAGGGAATGTCCTAGCTAGATTCATACGTAGACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000395
  • symbol: JSNZ_000395
  • description: methionine ABC transporter permease
  • length: 219
  • theoretical pI: 7.66251
  • theoretical MW: 23309.9
  • GRAVY: 1.15799

⊟Function[edit | edit source]

  • reaction:
    EC 7.4.2.11?  ExPASy
    non-specified enzyme name
  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 73.6)
    and 11 more
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 39.3)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 34)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 31.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 22)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 21.7)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 20.2)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.4)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.4)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 16.8)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 14.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 72.3)
    and 1 more
    FtsX; FtsX-like permease C-terminal (PF02687; HMM-score: 16.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001355
    • TAT(Tat/SPI): 0.001452
    • LIPO(Sec/SPII): 0.002101
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITRKQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYVAPYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLIGSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRRH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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