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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000335
  • pan locus tag?: SAUPAN001981000
  • symbol: ahpF
  • pan gene symbol?: ahpF
  • synonym:
  • product: alkyl hydroperoxide reductase subunit F

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000335
  • symbol: ahpF
  • product: alkyl hydroperoxide reductase subunit F
  • replicon: chromosome
  • strand: -
  • coordinates: 367197..368720
  • length: 1524
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTAATGCTGATTTAAAACAACAACTTAAACAATTATTAGAACTAATGGAGGGCAAC
    GTTGAATTCGTTGCCAGCCTTGGTTCAGATGAAAAATCTAAGGAACTCAAAGAATTATTA
    ACAGAAATTTCTGATATGTCACCTAGACTTTCACTTTCTGAAAAGCCTTTAAAACGTACA
    CCAAGTTTCTCAGTCAACCGTCCTGGCGAAGAAACAGGTGTAACATTTGCAGGAATTCCA
    TTAGGTCACGAGTTTAACTCACTTGTTTTAGCAATTTTACAGGTTAGTGGTCGTGCACCT
    AAAGAAAAACAGTCAATTATTGACCAAATTAAAAACTTAGAAGGTTCATTCCATTTTGAA
    ACATTCATTAGTTTAACGTGTCAAAAATGTCCTGATGTCGTTCAAGCACTTAACTTAATG
    AGTGTGATCAACCCTAACATCACGCATTCTATGATTGATGGTGCAGTGTTCCGTGAAGAA
    TCTGAAAACATCATGGCAGTCCCTGCTGTCTTTTTAAACGGCGAAGAATTTGGCAATGGT
    CGTATGACAATCCAAGATATTCTTTCGAAACTAGGCAGTACGGCAGATGCATCTGAGTTT
    GAAAATAAAGAACCTTATGATGTCTTAATCGTTGGTGGTGGTCCTGCTAGTGGTAGTGCA
    GCGATTTACACAGCACGTAAAGGTTTACGTACTGGTATAGTTGCTGATCGTATCGGTGGC
    CAAGTTAATGATACTGCTGGTATTGAGAACTTCATTACTGTTAAAGAAACAACTGGTTCT
    GAATTTTCTTCTAACTTAGCAGCGCACATTGATCAATATGACATTGATGCAATGACAGGT
    ATACGTGCTACAGATATCGAAAAGACTGACGAAGCAATTAAAGTTACGTTAGAAAACGGT
    GCTGTCTTAGAAAGTAAAACAGTCATTATTGCTACTGGTGCAGGTTGGCGTAAGCTAAAC
    ATTCCAGGTGAAGAGCAATTGATTAATAAAGGTGTTGCATTCTGCCCTCACTGTGACGGA
    CCTCTATTCGAAAATAAAGACGTAGCAGTTATCGGTGGCGGTAACTCTGGGGTTGAAGCA
    GCAATTGACCTTGCTGGTATCGTTAATCATGTTACATTATTCGAATTCGCTAGCGAATTA
    AAAGCAGACAACGTGTTACAAGATCGTTTACGTTCTTTATCAAATGTTGATATCAAAACA
    AATGCCAAAACTACTGAAGTTGTCGGAGAAGACCATGTTACAGGTATACGTTACGAAGAC
    ATGAGCACCGGCGAAGAACATCTACTTAACTTAGATGGTATCTTTGTTCAAATTGGTTTA
    CTTCCAAACACATCATGGTTAAAAGATGCTGTTGAATTAAACGAACGTGGTGAAATTGTT
    ATTGATCGTAACAATAATACTAACGTACCTGGAATATTTGCTGCTGGCGATGTCACAGAT
    CAGAAGAACAAACAAATTATCATTTCAATGGGCGCTGGTGCAAATGCAGCATTAAATGCC
    TTTGACTATATTATCAGAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1524

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000335
  • symbol: AhpF
  • description: alkyl hydroperoxide reductase subunit F
  • length: 507
  • theoretical pI: 4.41831
  • theoretical MW: 54717.4
  • GRAVY: -0.123077

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 812.9)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 812.9)
    and 41 more
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 306.7)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 278.2)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 78.4)
    glutaredoxin-like domain protein (TIGR02187; HMM-score: 73.9)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 73.3)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 71.3)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 57.5)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 53.5)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 50.3)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 45.9)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 44.5)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 42.7)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 41.2)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 34)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 33.9)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 31.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 31.3)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 30.1)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 29.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 25.8)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 24.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 21)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 20.7)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 17.8)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 17.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 17.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 16.9)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 16.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 16)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 14.7)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 14.5)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.2)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 12.5)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.1)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.1)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.1)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.8)
    salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 11.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02028; HMM-score: 11.1)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01811; EC 1.3.99.1; HMM-score: 9.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 171.1)
    and 19 more
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 89.3)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 54.1)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 31.8)
    Thioredoxin (CL0172) Thioredoxin_3; Thioredoxin domain (PF13192; HMM-score: 31)
    NADP_Rossmann (CL0063) HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 30.3)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 23)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 22.9)
    Thi4; Thi4 family (PF01946; HMM-score: 21.1)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 19)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 17.5)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 15.1)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 14.3)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 13.7)
    Thioredoxin (CL0172) Glutaredoxin; Glutaredoxin (PF00462; HMM-score: 13.2)
    no clan defined NNH3; NACHT N-terminal Helical domain 3 (PF22735; HMM-score: 12.6)
    NADP_Rossmann (CL0063) Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 12)
    Trp_halogenase; Tryptophan halogenase (PF04820; HMM-score: 11.9)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 11.3)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.982
    • Cytoplasmic Membrane Score: 0.0118
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0061
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00492
    • TAT(Tat/SPI): 0.003687
    • LIPO(Sec/SPII): 0.0011
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKPLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREESENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PerR (repression) regulon
    PerR(TF)important in Oxidative stress response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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