PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000324
• pan locus tag^?: SAUPAN001967000
• symbol: selX
• pan gene symbol^?: selX
• synonym:
• product: staphylococcal enterotoxin-like toxin X

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000324
• symbol: selX
• product: staphylococcal enterotoxin-like toxin X
• replicon: chromosome
• strand: +
• coordinates: 359990..360601
• length: 612
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGTTCAAAAAATATGACTCAAAAAATTCAATCGTATTAAAATCTATTCTATCGCTAGGT
  ATCATCTATTCGGGATCATTTGGAATATATCCAAAAGCAGACGCGTCAACACAAAATTCC
  TCAAGTGTACAAGATAAACAATTACAAAAAGTTGAAGAAGTACCAAATAATTCAGAAAAA
  GCTTTGGTTAAAAAACTTTACGATAGATACAGCAAGGATACAATAAATGGAAAATCTAAT
  AAATCTAGGAATTGGGTTTATTCAGAGAGACCTTTAAATGAAAACCAAGTTCGTATACAT
  TTAGAAGGTACATACACAGTTGCTGGCAGAGTGTATACACCTAAGAGGAACATTACTCTT
  AATAAAGAAGTTGTCACTTTAAAAGAATTGGATCATATCATAAGATTTGCTCATATTTCT
  TATGGCTTATATATGGGAGAACATTTGCCTAAAGGTAACATCGTCATAAATACAAAGAAT
  GGCGGTAAATATACATTAGAGTCGCATAAAGAGTTACAAAAGAATAGGGAAAATGTAGAA
  ATTAATACTGATGATATAAAAAATGTAACTTTCGAACTTGTGAAAAGTGTTAATGACATT
  GAACAAGTTTGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  612
  

⊟Protein

⊟General

• locus tag: JSNZ_000324
• symbol: SelX
• description: staphylococcal enterotoxin-like toxin X
• length: 203
• theoretical pI: 9.82726
• theoretical MW: 23225.2
• GRAVY: -0.680788

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Ubiquitin (CL0072) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 77.8)
  and 3 more
  Ice_binding (CL0847) DUF7305; Domain of unknown function (DUF7305) (PF23981; HMM-score: 18.2)
  E-set (CL0159) DUF7407; Domain of unknown function (DUF7407) (PF24156; HMM-score: 15.7)
  Ubiquitin (CL0072) CHIPS; Chemotaxis-inhibiting protein CHIPS (PF11434; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.002
  • Extracellular Score: 0.9979
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 35 aa
  • SP(Sec/SPI): 0.874029
  • TAT(Tat/SPI): 0.026589
  • LIPO(Sec/SPII): 0.010285
  • Cleavage Site: CS pos: 35-36. ADA-ST. Pr: 0.7963
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MFKKYDSKNSIVLKSILSLGIIYSGSFGIYPKADASTQNSSSVQDKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLEGTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKNGGKYTLESHKELQKNRENVEINTDDIKNVTFELVKSVNDIEQV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Gillian J Wilson, Keun Seok Seo, Robyn A Cartwright, Timothy Connelley, Olivia N Chuang-Smith, Joseph A Merriman, Caitriona M Guinane, Joo Youn Park, Gregory A Bohach, Patrick M Schlievert, W Ivan Morrison, J Ross Fitzgerald
A novel core genome-encoded superantigen contributes to lethality of community-associated MRSA necrotizing pneumonia.
PLoS Pathog: 2011, 7(10);e1002271
[PubMed:22022262] [WorldCat.org] [DOI] (I p)