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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000298
  • pan locus tag?: SAUPAN001883000
  • symbol: efeB
  • pan gene symbol?: fepB
  • synonym:
  • product: iron uptake transporter deferrochelatase/peroxidase subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000298
  • symbol: efeB
  • product: iron uptake transporter deferrochelatase/peroxidase subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 334645..335874
  • length: 1230
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAA
    ATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGG
    TCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCATATGAATTTTAT
    GGTAAAGTGCAACCAGGCATTACCACACCCACGCAAAAAACATGCAATTTCGTTGCGTTA
    GATTTGAAGTCAAAAGATAGAGATGCAATTAAGGCAATGTTTAAAAAGTGGACGGTTATG
    GCTGATCGTATGATGGATGGTGATACAGTTGGCAAGCCGAGTAACAATCCTTTAATGCCA
    CCAGTAGATACCGGTGAATCGATAGGATTAGGTGCAAGCAAGTTAACGATTACCTTTGGG
    ATTAGTAAGTCTTTGATGAAGAAAATTGGGTTATCTAGTAAAATTCCCGATGCCTTTAAA
    GATTTACCGCATTTTCCGAATGATCAGTTAATAGACGATTACAGCGATGGTGATATTATG
    ATTCAAGCATGCTCAAATGATTCGCAAGTATCCTTTCATGCGGTTCATAATTTAGTTCGT
    CCATTTCGAGATATTGTTAAGGTACGTTGGGCGCAATCTGGTTTTATCTCTGCTAAAGGT
    AAGGAAACACCTAGAAATTTAATGGCATTTAAAGATGGAACAATTAATCCTAGGAAGAAT
    AATCAACTTAAAGATTATGTGTTTATTGATGACGGATGGGCGAAACATGGAACTTATTGC
    GTTGTCAGACGTATTCAAATACACATTGAAACGTGGGATCGTACTGCACTGGAAGAACAA
    GAGGCTACATTTGGTCGGAAACGACATAGTGGTGCACCGTTAACAGGTGGGAAAGAGTTT
    GATGAAATTGACTTAAAAGCGAAAGATAGTCATGGCGAGTATATTATTGATAAAGATGCC
    CATACGAGGCTAGCGAAAGAAGCAAATACGTCAATTTTACGTAGAGCCTTTAATTATGTG
    GATGGTACGGATGACCGCACAGGTAACTTCGAAACAGGCTTACTTTTTATTGCTTTTCAA
    AAAGCGACAAAACAATTTATCGATATACAAAATAATTTAGGTAGTAATGATAAATTAAAT
    GAATATATTACACATAGAGGTTCTGCTTCATTTTTAGTATTACCAGGTGTTAGTAAGGGA
    GGATACCTTGGTGAAACATTATTTGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1230

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000298
  • symbol: EfeB
  • description: iron uptake transporter deferrochelatase/peroxidase subunit
  • length: 409
  • theoretical pI: 8.08612
  • theoretical MW: 45558.3
  • GRAVY: -0.480685

⊟Function[edit | edit source]

  • reaction:
    EC 4.98.1.1?  ExPASy
    non-specified enzyme name
  • TIGRFAM:
    Tat-translocated enzyme (TIGR01412; HMM-score: 598.6)
    and 3 more
    Dyp-type peroxidase family (TIGR01413; HMM-score: 330.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking Tat (twin-arginine translocation) pathway signal sequence (TIGR01409; HMM-score: 25.5)
    Unknown function General formate dehydrogenase region TAT target (TIGR02811; HMM-score: 13.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Dim_A_B_barrel (CL0032) Dyp_perox_C; Dyp-type peroxidase, C-terminal (PF20628; HMM-score: 175.8)
    Dyp_perox_N; Dyp-type peroxidase, N-terminal (PF04261; HMM-score: 166.1)
    and 2 more
    DUF7405; Family of unknown function (DUF7405) (PF24152; HMM-score: 24.2)
    TAT (CL0300) TAT_signal; TAT (twin-arginine translocation) pathway signal sequence (PF10518; HMM-score: 20)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helix: 1
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0249
    • Cytoplasmic Membrane Score: 0.4599
    • Cell wall & surface Score: 0.0038
    • Extracellular Score: 0.5115
  • LocateP:
  • SignalP: Signal peptide TAT(Tat/SPI) length 41 aa
    • SP(Sec/SPI): 0.002268
    • TAT(Tat/SPI): 0.988184
    • LIPO(Sec/SPII): 0.003962
    • Cleavage Site: CS pos: 41-42. MWS-FK. Pr: 0.3841
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKSMFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMADRMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFKDLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISAKGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTALEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILRRAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFLVLPGVSKGGYLGETLFD

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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