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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000268
  • pan locus tag?: SAUPAN001238000
  • symbol: JSNZ_000268
  • pan gene symbol?: nanT
  • synonym:
  • product: sodium:solute symporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000268
  • symbol: JSNZ_000268
  • product: sodium:solute symporter
  • replicon: chromosome
  • strand: -
  • coordinates: 303077..304609
  • length: 1533
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGTTATCATTTATCTACTAGCT
    ATGTTGTTCATTGGCGTTTATTTTACCAAGCGCGCGAGCCAAAGTACCAATAGTTTCTTT
    ACCGCAAGTGGTCGCTTGCCATCTTGGGTAGTTGGCTTTTCAATTTATGCTACTACGTTA
    AGTGCGATTACATTTATGTCGACACCAGAGAAAGCATTTTTAACAGATTGGTCATATATC
    GCTGGTAACATTGCTATCGTCGCAATTATTCCATTACTTATTTATTTCTATGTCCCTTTC
    TTTAAAAAGTTAAAGGTAACATCTGCATATGAATATTTAGAAGCTAGATTCGGCCCTAGC
    ATACGTGTTATTGGCTCATTATTATTTGTCGTTTATCATCTAGGGCGTGTAGCAATCGTT
    ATCTACTTACCAACATTAGCAATCACATCTGTATCGGACATGAACCCTTATATCGTTGCA
    TCACTCGTTGGTTTACTATGTATTTTATATACATTTTTAGGTGGATTCGAAGGTGTGGTT
    TGGAGTGATTTCATTCAAGGCGTCATTTTATTAGGCGGCGCTTTAGTTATCATTATTCTA
    GGTGTTATGAACATTAAAGGCGGTTTTGGCACTGTCTTTGCAGATGCGATTGAGCATAAA
    AAATTAATTAGTGCAGACAACTGGAAACTAAATACTGCAGCAGCTGCCATTCCAATTATT
    TTCCTAGGAAATATTTTCAACAACTTGTATCAATACACGGCGAGTCAAGACGTCGTGCAA
    CGTTATCAAGCTTCTGATAGTTTAAAAGAAACAAATAAATCGTTATGGACAAATGGTATC
    CTAGCTTTAATTTCAGCACCCTTATTTTATGGTATGGGTACAATGTTGTATTCATTTTAT
    GCACATGAAGCTGTTTTACCAAAAGGCTTCAATACATCATCTGTAGTGCCATATTTCATT
    TTGACTGAGATGCCACCATTTGTAGCAGGATTACTTATTGCAGCCATTTTCGCAGCTGCA
    CAGTCTACCATTTCATCTAGTTTAAATTCTATATCTGCTTGTATTTCAATCGACATTAAG
    CAACGCTTCTTCGGAAAAGGTAGCGAGCGACACGAAGTTAACTTTGCTCGTTTGGTCATC
    ATCATTGCAGGTATTTTCGGTTTTGGAATGTCACTATACTTAATTGCTTCTAATTCAAAT
    GACTTATGGGATTTATTCTTGTTTGTAACTGGATTATTCGGCGTTCCATTGGCTGGTGTA
    TTTGCAGTTGGTATTTTCACTAAACGTACGAATACATTCGGTGTTATTTGTGGATTAATA
    TTGGGTATCATCTTTGCTTACATCTATAATGGTGTTGGCAAAGGTAACTCACCTTTCTAT
    GTATCTACCATTTCATTTACAGTTGCTTTTGTCTTTGCTTATATACTTAGCTTCATTGTC
    CCTTCAAAACATAAAAAAGATATAACGGGATTAACAATTTTTGAAAAAGATAAACCATCA
    ACATACATTTCAAAAACGGCTACGAAAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1533

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000268
  • symbol: JSNZ_000268
  • description: sodium:solute symporter
  • length: 510
  • theoretical pI: 9.58689
  • theoretical MW: 55781.2
  • GRAVY: 0.733922

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 219.8)
    and 5 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 98.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 71.3)
    Metabolism Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 71.3)
    probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 58.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 33.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 140.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9993
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0006
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.043374
    • TAT(Tat/SPI): 0.001183
    • LIPO(Sec/SPII): 0.007584
  • predicted transmembrane helices (TMHMM): 13

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASGRLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFKKLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVASLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIEHKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLWTNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIAAIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARLVIIIAGIFGFGMSLYLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYIYNGVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTATKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NanR* (repression) regulon, CcpA regulon
    NanR*(TF)important in Sialic acid utilization, N-acetylglucosamine utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J Bacteriol: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)

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