PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000268
• pan locus tag^?: SAUPAN001238000
• symbol: JSNZ_000268
• pan gene symbol^?: nanT
• synonym:
• product: sodium:solute symporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000268
• symbol: JSNZ_000268
• product: sodium:solute symporter
• replicon: chromosome
• strand: -
• coordinates: 303077..304609
• length: 1533
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGTTATCATTTATCTACTAGCT
  ATGTTGTTCATTGGCGTTTATTTTACCAAGCGCGCGAGCCAAAGTACCAATAGTTTCTTT
  ACCGCAAGTGGTCGCTTGCCATCTTGGGTAGTTGGCTTTTCAATTTATGCTACTACGTTA
  AGTGCGATTACATTTATGTCGACACCAGAGAAAGCATTTTTAACAGATTGGTCATATATC
  GCTGGTAACATTGCTATCGTCGCAATTATTCCATTACTTATTTATTTCTATGTCCCTTTC
  TTTAAAAAGTTAAAGGTAACATCTGCATATGAATATTTAGAAGCTAGATTCGGCCCTAGC
  ATACGTGTTATTGGCTCATTATTATTTGTCGTTTATCATCTAGGGCGTGTAGCAATCGTT
  ATCTACTTACCAACATTAGCAATCACATCTGTATCGGACATGAACCCTTATATCGTTGCA
  TCACTCGTTGGTTTACTATGTATTTTATATACATTTTTAGGTGGATTCGAAGGTGTGGTT
  TGGAGTGATTTCATTCAAGGCGTCATTTTATTAGGCGGCGCTTTAGTTATCATTATTCTA
  GGTGTTATGAACATTAAAGGCGGTTTTGGCACTGTCTTTGCAGATGCGATTGAGCATAAA
  AAATTAATTAGTGCAGACAACTGGAAACTAAATACTGCAGCAGCTGCCATTCCAATTATT
  TTCCTAGGAAATATTTTCAACAACTTGTATCAATACACGGCGAGTCAAGACGTCGTGCAA
  CGTTATCAAGCTTCTGATAGTTTAAAAGAAACAAATAAATCGTTATGGACAAATGGTATC
  CTAGCTTTAATTTCAGCACCCTTATTTTATGGTATGGGTACAATGTTGTATTCATTTTAT
  GCACATGAAGCTGTTTTACCAAAAGGCTTCAATACATCATCTGTAGTGCCATATTTCATT
  TTGACTGAGATGCCACCATTTGTAGCAGGATTACTTATTGCAGCCATTTTCGCAGCTGCA
  CAGTCTACCATTTCATCTAGTTTAAATTCTATATCTGCTTGTATTTCAATCGACATTAAG
  CAACGCTTCTTCGGAAAAGGTAGCGAGCGACACGAAGTTAACTTTGCTCGTTTGGTCATC
  ATCATTGCAGGTATTTTCGGTTTTGGAATGTCACTATACTTAATTGCTTCTAATTCAAAT
  GACTTATGGGATTTATTCTTGTTTGTAACTGGATTATTCGGCGTTCCATTGGCTGGTGTA
  TTTGCAGTTGGTATTTTCACTAAACGTACGAATACATTCGGTGTTATTTGTGGATTAATA
  TTGGGTATCATCTTTGCTTACATCTATAATGGTGTTGGCAAAGGTAACTCACCTTTCTAT
  GTATCTACCATTTCATTTACAGTTGCTTTTGTCTTTGCTTATATACTTAGCTTCATTGTC
  CCTTCAAAACATAAAAAAGATATAACGGGATTAACAATTTTTGAAAAAGATAAACCATCA
  ACATACATTTCAAAAACGGCTACGAAAAAGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1533
  

⊟Protein

⊟General

• locus tag: JSNZ_000268
• symbol: JSNZ_000268
• description: sodium:solute symporter
• length: 510
• theoretical pI: 9.58689
• theoretical MW: 55781.2
• GRAVY: 0.733922

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 219.8)
  and 5 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 98.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 71.3)
  Metabolism Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 71.3)
  probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 58.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 33.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 140.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9993
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0006
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.043374
  • TAT(Tat/SPI): 0.001183
  • LIPO(Sec/SPII): 0.007584
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASGRLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFKKLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVASLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIEHKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLWTNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIAAIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARLVIIIAGIFGFGMSLYLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYIYNGVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTATKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulators: NanR* (repression) regulon, CcpA regulon

  NanR*	(TF)	important in Sialic acid utilization, N-acetylglucosamine utilization; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)
  CcpA	(TF)	important in Carbon catabolism; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J Bacteriol: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)