PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000215
• pan locus tag^?: SAUPAN001161000
• symbol: JSNZ_000215
• pan gene symbol^?: —
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000215
• symbol: JSNZ_000215
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 254150..255526
• length: 1377
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  GTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCA
  TTAACATATTGGTTGTTTGCACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACG
  TATCAAACCTCTCCTGCCGTGTTAAATTTATCTATTAGTTTAACTTCCTTCGCCACAGGT
  ATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGAATGACATAC
  ATGGGTCTCATAATCAGTATGGTTGCATCTCTTCTATTAATTATATCGGACATCACTGCA
  CTGCTTATCATCGGCAGAATTTTACAAGGTTTATCAGCAGCTATCTTGTTACCTTCAACA
  GTTGGCGTTTTAAATAATCAATTTAAAGTAGAACATTTAAGACGAGCGATTAGTTATCTA
  ATGATTAGTACTGTTGGTGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTAATTGCCACA
  AATTTCGGATGGCAAACGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTG
  CTTCTAAAAGGCACACCTGAAAAAGTAAGTCAACATAGCCACCGTCATCCATTCGATTAC
  AAAGGTATGTCGATTTTCGCTGTTATGATTGGTAGCTTTACATTATTGTTAACACAAGGA
  TTCGAACAAGGTTGGTTTAGTACATTTTCATTCATTTGTCTGAGCATTTTTATCATCACT
  ACATTAATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTA
  TTACGCAACCGTCCGTTCATTGGTGCATTTTTAAATAACTTTGTTTTAAATAGTGGTCTA
  GGCGTAACAGTGGTCTTTTTCATATTTGCTCAAACACACCTTGGTTTATCAGCTGCGCAA
  TCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGTTTAGGCGAA
  AAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTC
  ATCGGCATCACTATTATTAGCATGACATCACTTCAACCATCACAATATATCATTGCTGTT
  GTTATTGGGTTTGTCATTTGCGCAATTGGAAATGGTTTAGTCGCAACACCCGGCCTTACA
  ATTGCAATTTTCAGTATGCCAAATGAAAAAGTTGGTTTAGCTACAGGATTATATAAAATG
  AGTGGTACATTAGGTGGCGCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTA
  CAACTAAACTATGCACCAAGTGTAGCTGCAACCGTAACATTTATCGTCAGCATTGTATTG
  ATGATCCTTGGCTCATTGTCTGCATACATGATCATTCCAAAAACGGTTAAATCTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1377
  

⊟Protein

⊟General

• locus tag: JSNZ_000215
• symbol: JSNZ_000215
• description: MFS transporter
• length: 458
• theoretical pI: 10.2222
• theoretical MW: 49066.4
• GRAVY: 0.981659

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 129.4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 129.4)
  and 8 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 55)
  multidrug resistance protein (TIGR00880; HMM-score: 45.3)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 41.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 33.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 29.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 26.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 18.1)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 16.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 139.4)
  and 1 more
  no clan defined UL42; HCMV UL42 (PF17638; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.001
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.123294
  • TAT(Tat/SPI): 0.003305
  • LIPO(Sec/SPII): 0.01869
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMVASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKVEHLRRAISYLMISTVGGIGLAGVIGGLIATNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIFAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications