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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000207
  • pan locus tag?: SAUPAN001152000
  • symbol: JSNZ_000207
  • pan gene symbol?: —
  • synonym:
  • product: methyltransferase domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000207
  • symbol: JSNZ_000207
  • product: methyltransferase domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 248765..249526
  • length: 762
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCTAAAGAAGCTGGTCATACATTTTTAGCTAAATTAGGAAAAACTCGTCTACGCCCC
    GGTGGTAAAGAAGCAACAGATTGGTTAATACAACAAGGGGCATTTTCACAAGATAAACAA
    GTGTTAGAAGTGGCATGTAATATGTGCACAACATCTATTTATCTAGCTCATACATATGGC
    TGTCACATTCAAGGCGTTGATATAAATAAGAAAGCATTAGAAAAAGCACAGGAAAACATT
    TCAGCAGCAGGTCTTGAATCATATATTCAAGTTCAACAAGCGAATGCTGTTAAATTGCCC
    TTTGATGACAATCAATTCGATATCGTTTTAAATGAAGCAATGTTAACAATGTTACCCATC
    GCCATAAAGGAAAAAGCATTACGCGAGTACTACCGAGTCTTAAAGCCTGGGGGTATCTTG
    TTAACACATGATATTGTCATCGTTAATGAATCACATGCCACACATGTTGTTAAATCATTA
    TCTGCAGCAATTAATGTCAATGTCTCACCGCAGACGAAACTTGGCTGGTTAGATTTATAT
    AATCAAGCTGGTTTTAATCATGTGCATTATCATACTGGTCCAATGAGTTTAATGACACCA
    AAAGGTTTAATTTATGACGAAGGTATTGTTGGAACTATAAAGATTATCAACAATGCTTTG
    AAAAAAGAAAATCGACCAATGTTTTGTAAAATGTTTAAAACGATGACTAAATTGCGTAAA
    GATATGAATTATATTACTTTTGTCGCTAAAAAAGAGCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    762

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000207
  • symbol: JSNZ_000207
  • description: methyltransferase domain-containing protein
  • length: 253
  • theoretical pI: 9.42825
  • theoretical MW: 28346.8
  • GRAVY: -0.183399

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 58.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 48.9)
    and 14 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 32.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 31.6)
    pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 23.9)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 23.6)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 22.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 21.9)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 21.6)
    methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 19)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 18.5)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 18.3)
    Unknown function Enzymes of unknown specificity tRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 16.5)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 13.5)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 13.4)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 12.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 74.5)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 73.6)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 68.5)
    and 17 more
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 55.8)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 41.7)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 34.6)
    UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 30.2)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 27.8)
    Anamorsin_N; Anamorsin, N-terminal (PF20922; HMM-score: 26.3)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 23.4)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 23.4)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 22.4)
    CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 19.7)
    Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 18.4)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 16.6)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 16)
    TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 15.4)
    TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 13.3)
    DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 12.9)
    Methyltransf_10; RNA methyltransferase (PF05971; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.4061
    • Cytoplasmic Membrane Score: 0.59
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0037
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.077335
    • TAT(Tat/SPI): 0.001947
    • LIPO(Sec/SPII): 0.0036
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEVACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFDDNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSLSAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINNALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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