From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000181
  • pan locus tag?: SAUPAN001110000
  • symbol: JSNZ_000181
  • pan gene symbol?: glcC
  • synonym:
  • product: PTS transporter subunit EIIC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000181
  • symbol: JSNZ_000181
  • product: PTS transporter subunit EIIC
  • replicon: chromosome
  • strand: -
  • coordinates: 219370..220899
  • length: 1530
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATCTTTATTTGAAAAAGCACAGCAGTTCGGCAAGTCCTTTATGCTACCTATCGCA
    ATCTTACCAGCTGCAGGTCTATTGTTGGGTATCGGTGGTGCATTAAGTAATCCAAACACC
    GTTAAAGCATACCCTATTTTAGATATTACCTTATTACAAAATATTTTTACATTAATGTCA
    GCTGCAGGTAGTATTGTTTTCCAAAATTTACCAGTCATCTTTGCAATTGGTGTCGCAATC
    GGATTATCTAGAAGCGATAAAGGTACTGCAGGTTTAGCTGCACTACTCGGTTTCTTAATT
    ATGAACGCAACTATGAATGGCTTATTAACTATCACGGGCACATTGGCAAAAGATCAGCTT
    GCACAAAATGGACAAGGCATGGTGCTCGGTATACAAACGGTTGAAACCGGTGTTTTTGGT
    GGGATTATCACAGGTATTATGACCGCAATACTTCACAACAAATATCACAAAGTAGTATTA
    CCACCGTATTTAGGTTTCTTTGGTGTCTCTAGATTTGTCCCTATTGTCACAGCATTTGCC
    GCAATCTTTTTAGGTGTATTGATGTTTTTCATTTGGCCAAGCATACAAGCCGGCATTTAT
    CATGTTGGTGGATTTGTAACGAAAACAGGTGCCATCGGTACTTTTGTTTATGGCTTCATC
    TTAAGATTGTTAGGTCCACTCGGTTTACATCATATTTTTTACTTACCGTTTTGGCAGACG
    GCACTTGGTGGTACTTTAGAAGTCAAAGGGCACTTAGTTCAAGGTACGCAGAACATCTTC
    TTTGCTCAACTTGGTGATCCAGATGTGACGAAGTATTATTCAGGTGTGTCACGCTTTATG
    TCAGGCCGTTTTATTACGATGATGTTCGGCTTATGTGGTGCCGCACTTGCAATTTATCAC
    ACAGCTAAACCTGAACATAAAAAAGTTGTCGGCGGTTTAATGTTATCCGCTGCACTCACT
    TCATTTTTAACAGGTATTACCGAACCTTTAGAGTTTAGTTTCTTGTTTGTCGCACCTATT
    CTTTATGTAATCCATGCCTTCTTTGATGGATTAGCATTTATGATGGCAGACATTTTCAAC
    ATTACAATTGGTCAAACCTTCAGTGGAGGCTTTATCGATTTCTTACTCTTTGGTGTGCTA
    CAAGGTAATAGTAAAACAAACTACCTATACGTCATACCTATTGGAATTGTGTGGTTCTGT
    TTGTATTACATCGTTTTCAGATTCTTAATTACGAAATTTAATTTCAAAACACCTGGTCGA
    GAAGATAAAGCTGCAGCACAACAAGTTGAGGCTACTGAAAGAGCACAAACTATTGTTGCT
    GGTTTGGGAGGCAAAGATAACATTGAAATCGTTGACTGTTGTGCAACGAGACTACGCGTC
    ACACTTCATCAAAATGACAAAGTCGATAAAGTATTACTCGAAAGTACTGGTGCCAAAGGT
    GTAATCCAGCAAGGCACTGGTGTGCAAGTAATTTATGGGCCCCACGTTACAGTTATCAAA
    AATGAAATTGAAGAATTGCTCGGGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000181
  • symbol: JSNZ_000181
  • description: PTS transporter subunit EIIC
  • length: 509
  • theoretical pI: 8.96532
  • theoretical MW: 54975.4
  • GRAVY: 0.622593

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 587)
    and 11 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 459.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 445.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 309.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 283.5)
    Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 283.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 264.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 69.5)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 46.9)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 44.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 295.3)
    and 1 more
    no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 52.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 43 aa
    • SP(Sec/SPI): 0.578981
    • TAT(Tat/SPI): 0.088154
    • LIPO(Sec/SPII): 0.051874
    • Cleavage Site: CS pos: 43-44. VKA-YP. Pr: 0.4433
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAYPILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMNATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVLPPYLGFFGVSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYGFILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGVSRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSFLFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVIPIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEIVDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLGD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NanR* (repression) regulon
    NanR*(TF)important in Sialic acid utilization, N-acetylglucosamine utilization; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026) 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_000181&oldid=105822"