From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000115
  • pan locus tag?: SAUPAN001011000
  • symbol: JSNZ_000115
  • pan gene symbol?: ssuA
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000115
  • symbol: JSNZ_000115
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 131973..132947
  • length: 975
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAGGTTAAGCATAATCGTCATCATTGGAATCTTTATAATTACAGGATGTGATTGG
    CAAAGGACGTCTAAAGAACCGTCTAAAAATGCCCAAAATCAGCAAGTGATTAAAATTGGA
    TATTTGCCGATTACACATTCAGCTAATTTGATGATGACTAAAAAATTATTATCACAATAC
    AATCATCCGAAATATAAACTAGAATTAGTTAAATTCAATAATTGGCCAGATTTAATGGAC
    GCATTAAACAGTGGTCGTATTGATGGTGCATCAACTTTAATAGAGCTAGCGATGAAATCA
    AAACAGAAGGGCTCAAATATAAAGGCTGTGGCATTGGGCCATCATGAAGGCAATGTCATT
    ATGGGACAAAAAGGTATGCACTTAAATGAATTTAATAATAATGGCGATGATTACCATTTT
    GGTATACCACATCGTTATTCAACACATTATCTTTTACTTGAGGAATTACGTAAACAATTA
    AAGATTAAACCGGGGCATTTTAGCTATCATGAAATGTCGCCAGCAGAAATGCCAGCCGCA
    TTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAA
    AAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGC
    TGTGTGCTAGTACTGAGAGGGGAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTT
    GTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAGTGTAGACATT
    ATGACGCATCATTTTAAACAAAGTCGTGACGTTTTAACACAGTCAGCGGCATGGACATCC
    TATGGTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAA
    CATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAG
    GCATCGCGTTCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000115
  • symbol: JSNZ_000115
  • description: ABC transporter substrate-binding protein
  • length: 324
  • theoretical pI: 9.26569
  • theoretical MW: 36681.8
  • GRAVY: -0.481173

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other ABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 77.6)
    and 2 more
    taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 22.5)
    Metabolism Transport and binding proteins Anions phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 14.2)
  • TheSEED: data available for N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PBP (CL0177) NMT1_2; NMT1-like family (PF13379; HMM-score: 79.7)
    and 3 more
    OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 24.9)
    Lipoprotein_9; NlpA lipoprotein (PF03180; HMM-score: 17.7)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0043
    • Cytoplasmic Membrane Score: 0.6519
    • Cell wall & surface Score: 0.0052
    • Extracellular Score: 0.3386
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000636
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.998548
    • Cleavage Site: CS pos: 17-18. ITG-CD. Pr: 0.9996
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPITHSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQKGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQLKIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDAYCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSAAWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_000115&oldid=105756"