PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000016
• pan locus tag^?: SAUPAN000026000
• symbol: dnaB
• pan gene symbol^?: dnaC
• synonym:
• product: replicative DNA helicase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000016
• symbol: dnaB
• product: replicative DNA helicase
• replicon: chromosome
• strand: +
• coordinates: 20246..21646
• length: 1401
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGGATAGAATGTATGAGCAAAATCAAATGCCACATAACAATGAAGCTGAACAGTCTGTC
  TTAGGTTCAATTATTATTGATCCAGAATTGATTAATACTACTCAGGAAGTGTTGCTTCCT
  GAGTCGTTTTATAGGGGTGCCCATCAACATATTTTCCGTGCAATGATGCACTTAAATGAA
  GATAACAAAGAAATTGATGTAGTAACATTGATGGATCAGTTATCAAGTGAAGGTACGTTG
  AATGAAGCGGGTGGCCCGCAATATCTTGCAGAGTTATCTACAAATGTACCAACGACGCGA
  AATGTTCAGTATTATACTGATATCGTTTCTAAGCATGCATTAAAACGTAGATTGATTCAA
  ACTGCAGATAGTATTGCCAATGATGGATATAATGATGAACTTGAACTAGATGCGATTTTA
  AGTGATGCAGAACGTCGAATTTTAGAGCTGTCATCTTCTCGTGAAAGCGATGGCTTTAAA
  GACATTCGAGACGTCTTAGGACAAGTGTATGAAACAGCTGAAGAGCTTGATCAAAATAGT
  GGTCAAACACCAGGTATACCTACAGGGTATCGAGATTTAGACCAAATGACAGCAGGGTTC
  AACCGAAATGATTTAATTATCCTTGCAGCGCGTCCATCTGTAGGTAAGACTGCGTTCGCA
  CTTAATATTGCACAAAAAGTTGCAACGCATGAAGATATGTATACAGTTGGTATTTTCTCA
  CTAGAGATGGGTGCTGATCAGTTAGCCACACGTATGATTTGTAGTTCTGGTAATGTTGAC
  TCAAACCGCTTAAGAACGGGTACTATGACTGAGGAAGATTGGAGTCGTTTTACTATAGCG
  GTAGGTAAATTATCACGTACGAAGATTTTTATTGATGATACACCGGGTATTCGAATTAAT
  GATTTACGTTCTAAATGTCGTCGATTAAAGCAAGAACATGGCTTAGACATGATTGTGATT
  GACTACTTACAGTTGATTCAAGGTAGTGGTTCACGTGCGTCCGATAACAGACAACAGGAA
  GTTTCTGAAATCTCTCGTACATTAAAAGCATTAGCCCGTGAATTAGAATGTCCAGTTATC
  GCATTAAGTCAGTTATCTCGTGGTGTTGAACAACGACAAGATAAACGTCCAATGATGAGT
  GATATTCGTGAATCTGGTTCGATTGAGCAAGATGCTGATATCGTTGCGTTCTTATACCGT
  GATGATTACTATAACCGTGGTGGCGATGAAGATGATGACGATGATGGTGGTTTCGAGCCA
  CAAACGAATGATGAAAACGGTGAAATTGAAATTATCATTGCTAAGCAACGTAACGGTCCA
  ACAGGCACAGTTAAGTTACACTTTATGAAACAATATAATAAATTTACCGATATCGATTAT
  GCACATGCAGATATGATGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1401
  

⊟Protein

⊟General

• locus tag: JSNZ_000016
• symbol: DnaB
• description: replicative DNA helicase
• length: 466
• theoretical pI: 4.41767
• theoretical MW: 52557.2
• GRAVY: -0.590987

⊟Function

• reaction:
  EC 3.6.4.12?  ExPASy
  DNA helicase ATP + H₂O = ADP + phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 585.5)
  and 3 more
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 448.8)
  Genetic information processing Protein synthesis tRNA aminoacylation L-seryl-tRNA(Sec) kinase (TIGR03574; EC 2.7.1.164; HMM-score: 12.8)
  arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 12.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 383.1)
  and 8 more
  no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 119.3)
  P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 53.7)
  divDNAB; Divergent DnaB-like ATPase (PF20307; HMM-score: 18.9)
  ATPase; KaiC (PF06745; HMM-score: 15.6)
  AB_hydrolase (CL0028) Lipase; Lipase (PF00151; HMM-score: 13.3)
  P-loop_NTPase (CL0023) PSY3; Shu complex component Psy3, DNA-binding description (PF16836; HMM-score: 13.3)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.6)
  AAA_24; AAA domain (PF13479; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9109
  • Cytoplasmic Membrane Score: 0.0705
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0184
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004964
  • TAT(Tat/SPI): 0.000152
  • LIPO(Sec/SPII): 0.000264
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFYRGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSSEGTLNEAGGPQYLAELSTNVPTTRNVQYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFKDIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTAFALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSRFTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRASDNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMKQYNKFTDIDYAHADMM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications