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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12490 [old locus tag: SA2176 ]
  • pan locus tag?: SAUPAN005915000
  • symbol: SA_RS12490
  • pan gene symbol?: narK
  • synonym:
  • product: nitrate transporter NarT

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12490 [old locus tag: SA2176 ]
  • symbol: SA_RS12490
  • product: nitrate transporter NarT
  • replicon: chromosome
  • strand: -
  • coordinates: 2444291..2445460
  • length: 1170
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2444291..2445460) NCBI
  • BioCyc: SA_RS12490 BioCyc
  • MicrobesOnline: see SA2176

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGG
    TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
    ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCAGTGCTCCGT
    GTGCCATTTGGTTATTTAACAAATATTGTTGGTGCTAAATGGGTATTCTTTACAAGTTTT
    ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACGCCGGGTATGTTAATG
    GCATCAGGATTTTTCCTTGGCGTAGGTGGCGCAATTTTCTCTGTTGGTGTTACATCAGTT
    CCTAAATATTTCCCAAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGGAAT
    ATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
    ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTT
    GGTGACACACAAGAACGTAAAATTAAAGTGCCATTAATGGCCCAAATGAAAACATTATCT
    AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTCATTACTTTTGGTGCTTTTGTA
    GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
    GCTGGTATTCGTTCAGGTGTATTCATTGCGCTGGCAACATTCTTGAGACCAATAGGTGGT
    ATACTAGGTGATAAATTTAATGCAGTTAAAGTACTGATGATTGATTTTGTTATTATGATT
    ATCGGTGCCGTTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
    ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATTTTTAAATTAGTACCATCCTAC
    TTCTCAAATGAAGCAGGATCAGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
    TTCTTCCCACCGTTGGTCATCACATACGTAGCTAATTTAACAGGTTCAAGCCATTTAGCA
    TTTATTTTCTTAGCGGTATTTGGATGCATTGCATTATTTACCATGCGCCATTTATATCAA
    AAAGAATATGGTTCATTGAAACACAGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1170

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS12490 [old locus tag: SA2176 ]
  • symbol: SA_RS12490
  • description: nitrate transporter NarT
  • length: 389
  • theoretical pI: 9.92595
  • theoretical MW: 42175.2
  • GRAVY: 0.909769

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 199.8)
    and 9 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 60.7)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 59.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 53)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 46.7)
    multidrug resistance protein (TIGR00880; HMM-score: 38.4)
    oxalate/formate antiporter (TIGR04259; HMM-score: 24.6)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.8)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.8)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 16.2)
  • TheSEED: see SA2176
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 152.5)
    and 3 more
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 18.8)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 11.5)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 8.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070673
    • TAT(Tat/SPI): 0.001376
    • LIPO(Sec/SPII): 0.020064
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: NreC*, Rex* see SA2176

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]