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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS09970 [old locus tag: SA1736 ]
  • pan locus tag?: SAUPAN004956000
  • symbol: SA_RS09970
  • pan gene symbol?: aldH
  • synonym:
  • product: aldehyde dehydrogenase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS09970 [old locus tag: SA1736 ]
  • symbol: SA_RS09970
  • product: aldehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1988703..1990082
  • length: 1380
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1988703..1990082) NCBI
  • BioCyc: SA_RS09970 BioCyc
  • MicrobesOnline: see SA1736

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATATCATTGAGCAAAAATTTTATGACAGTAAAGCTTTTTTCAATACACAACAAACT
    AAAGATATTAGTTTTAGAAAAGATCAATTAAAGAAGTTAAGCAAAGCTATTAAATCATAC
    GAGAGCGATATTTTAGAAGCACTATATACAGATTTAGGAAAAAATAAAGTCGAAGCTTAT
    GCTACTGAAATTGGCATAACTTTGAAAAGTATCAAAAATGCCCGTAAGGAACTTAAAAAC
    TGGACTAAAACAAAAAATGTAGACACACCTTTATATTTATTTCCAACAAAAAGCTATATC
    AAAAAAGAACCTTATGGAACAGTTTTGATCATTGCACCATTTAACTATCCTTTTCAACTA
    GTATTCGAACCTTTAATCGGTGCTATTGCAGCAGGTAATACAGCAATTATTAAACCATCT
    GAGTTGACACCAAATGTTGCACGAGTGATTAAACGATTAATCAATGAAACATTTGATGCA
    AATTACATTGAAGTTATTGAGGGAGGAATTGAAGAAACGCAAACGTTAATTCACTTACCT
    TTTGACTATGTCTTCTTTACAGGAAGTGAAAATGTAGGCAAAATCGTTTATCAAGCTGCC
    AGCGAAAATTTAGTTCCTGTGACATTAGAAATGGGTGGTAAATCTCCAGTCATCGTTGAT
    GAAACAGCGAATATTAAAGTTGCTAGTGAGCGCATTTGTTTTGGGAAATTCACTAATGCC
    GGCCAAACATGTGTTGCACCAGATTACATTTTAGTACACGAATCTGTAAAAGATGATTTA
    ATCACAGCCCTATCAAAAACGTTGCGTGAATTTTATGGTCAAAATATACAACAAAGTCCA
    GATTATGGCCGCATTGTAAACCTTAAACATTATCATCGTCTGACTTCATTACTTAACAGT
    GCACAAATGAATATTGTATTTGGTGGTCATAGTGATGAGGATGAACGTTATATAGAACCA
    ACATTGTTAGATCACGTTACAAGTGATTCAGCAATTATGCAAGAAGAAATTTTTGGTCCT
    ATCTTACCGATTTTAACGTATCAGTCATTGGATGAAGCAATAGCCTTTATTCACCAAAGA
    CCAAAACCTTTGAGTTTATATTTATTTAGCGAAGATGAAAATGCTACACAACGTGTAATA
    AACGAGTTATCATTTGGCGGCGGCGCTATTAATGATACATTGATGCACCTAGCGAATCCT
    AAATTACCATTTGGTGGTGTTGGCGCCTCAGGTATGGGACGCTATCATGGTAAATATTCA
    TTCGACACTTTTACACATGAAAAAAGCTACATTTTCAAATCTACACGATTAGAATCAGGT
    GTCCATTTACCACCATATAAAGGTAAATTTAAATACATCAAAGCTTTCTTTAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS09970 [old locus tag: SA1736 ]
  • symbol: SA_RS09970
  • description: aldehyde dehydrogenase
  • length: 459
  • theoretical pI: 7.12628
  • theoretical MW: 51727.8
  • GRAVY: -0.236166

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 209.1)
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 199.7)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 187.5)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 181.6)
    and 14 more
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 166.4)
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 153.8)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 153.3)
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 145.6)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 145.4)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 121.6)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 99.6)
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 91.8)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 71.9)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 45.6)
    Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 25.2)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 21.2)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 16.2)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AB (TIGR02833; HMM-score: 11.8)
  • TheSEED: see SA1736
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 318.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003734
    • TAT(Tat/SPI): 0.001262
    • LIPO(Sec/SPII): 0.000349
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNIIEQKFYDSKAFFNTQQTKDISFRKDQLKKLSKAIKSYESDILEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKKEPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDANYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVIVDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNIQQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQEEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAINDTLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKFKYIKAFFKN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS07290(asnC)asparagine--tRNA ligase  [1] (data from MRSA252)
    SA_RS04915enoyl-ACP reductase  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SA_RS08600universal stress protein  [1] (data from MRSA252)
    SA_RS11635DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]