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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05135 [old locus tag: SA0905 ]
  • pan locus tag?: SAUPAN003260000
  • symbol: SA_RS05135
  • pan gene symbol?: atl
  • synonym: atlA
  • product: bifunctional autolysin

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS05135 [old locus tag: SA0905 ]
  • symbol: SA_RS05135
  • product: bifunctional autolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 1026342..1030088
  • length: 3747
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1026342..1030088) NCBI
  • BioCyc: SA_RS05135 BioCyc
  • MicrobesOnline: see SA0905

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
    GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
    AATGTTTTAGATAGTAATAAAGTTAAAGCAACTACTGAACAAGCAAAAGCTGAGGTAAAA
    AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
    AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
    GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
    AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
    TTAGTTGATGATGAAGATGATAATTCAGAACATCAAATTAATCCAGAATTAATTAAATCA
    GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAGCCGCAGCACCTAAAGCTAAAACT
    GAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCAGCACAACCAAGATCAGTTGCTGCA
    ACACCAAAAACGAGTTTGCCAAAATATAAACCACAAGTAAACTCTTCAATTAACGATTAC
    ATTCGTAAAAATAACTTAAAAGCACCTAAAATTGAAGAAGATTATACATCTTACTTCCCT
    AAATACGCATACCGTAACGGCGTAGGTCGTCCTGAAGGTATCGTAGTTCATGATACAGCT
    AATGATCGTTCGACGATAAATGGTGAAATTAGTTATATGAAAAATAACTATCAAAACGCA
    TTCGTACATGCATTTGTTGATGGGGATCGTATAATCGAAACAGCACCAACGGATTACTTA
    TCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTCATCAATGTTGAAATCGTACACACA
    CACGACTATGCTTCATTTGCACGTTCAATGAATAACTATGCTGACTATGCAGCTACACAA
    TTACAATATTATGGTTTAAAACCAGACAGTGCTGAGTATGATGGAAATGGTACAGTATGG
    ACTCACTACGCTGTAAGTAAATATTTAGGTGGTACGGACCATGCCGATCCACATGGATAT
    TTAAGAAGTCATAATTATAGTTATGATCAATTATATGACTTAATTAATGAAAAATATTTA
    ATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAATTTACAACTACCCCTACTACACCA
    TCAAAACCAACAACACCGTCGAAACCATCAACTGGTAAATTAACAGTTGCAGCAAACAAT
    GGTGTCGCACAAATCAAACCAACAAATAGTGGTTTATATACTACTGTTTACGACAAAACT
    GGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCTGTATCTAAAACAGCTACATTAGGT
    AATCAAAAATTCTATCTTGTTCAAGATTACAATTCTGGTAATAAATTTGGTTGGGTTAAA
    GAAGGCGATGTGGTTTACAACACAGCTAAATCACCTGTAAATGTAAATCAATCATATTCA
    ATCAAATCTGGTACGAAACTTTATACAGTACCTTGGGGTACATCTAAACAAGTTGCTGGT
    AGCGTGTCTGGCTCTGGAAACCAAACATTTAAGGCTTCAAAGCAACAACAAATTGATAAA
    TCAATTTATTTATATGGCTCTGTGAATGGTAAATCTGGTTGGGTAAGTAAAGCATATTTA
    GTTGATACTGCTAAACCTACGCCTACACCAATACCTAAGCCATCAACACCTACAACAAAT
    AATAAATTAACAGTTTCATCATTAAACGGTGTTGCTCAAATTAATGCTAAAAACAATGGC
    TTATTCACTACAGTTTATGACAAAACTGGTAAGCCAACGAAAGAAGTTCAAAAAACATTT
    GCTGTAACAAAAGAAGCAAGTTTAGGTGGAAACAAATTCTACTTAGTTAAAGATTACAAT
    AGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGACGTTATTTATAACAATGCAAAATCA
    CCTGTAAATGTAATGCAAACATATACAGTAAAACCAGGCACTAAATTATATTCAGTACCT
    TGGGGCACTTATAAACAAGAAGCTGGTGCAGTTTCTGGTACAGGTAACCAAACTTTTAAA
    GCGACTAAGCAACAACAAATTGATAAATCTATCTATTTATTTGGAACTGTAAATGGTAAA
    TCTGGTTGGGTAAGTAAAGCATATTTAGCTGTACCTGCTGCACCTAAAAAAGCAGTAGCA
    CAACCAAAAACAGCTGTAAAAGCTTATACTGTTACTAAACCACAAACGACTCAAACAGTT
    AGCAAGATTGCTCAAGTTAAACCAAACAACACTGGTATTCGTGCTTCTGTTTATGAAAAA
    ACAGCGAAAAACGGTGCGAAATATGCAGACCGTACGTTCTATGTAACAAAAGAGCGTGCT
    CATGGTAATGAAACGTATGTATTATTAAACAATACAAGCCATAACATCCCATTAGGTTGG
    TTCAATGTAAAAGACTTAAATGTTCAAAACCTAGGCAAAGAAGTTAAAACGACTCAAAAA
    TATACTGTTAATAAATCAAATAACGGCTTATCAATGGTTCCTTGGGGTACTAAAAACCAA
    GTCATTTTAACAGGCAATAACATTGCTCAAGGTACATTTAATGCAACGAAACAAGTATCT
    GTAGGCAAAGATGTTTATTTATACGGTACTATTAATAACCGCACTGGTTGGGTAAATGCA
    AAAGATTTAACTGCACCAACTGCTGTGAAACCAACTACATCAGCTGCCAAAGATTATAAC
    TACACTTATGTAATTAAAAATGGTAATGGTTATTACTATGTAACACCAAATTCTGATACA
    GCTAAATACTCATTAAAAGCATTTAATGAACAACCATTCGCAGTTGTTAAAGAACAAGTC
    ATTAATGGACAAACTTGGTACTATGGTAAATTATCTAACGGTAAATTAGCATGGATTAAA
    TCAACTGATTTAGCTAAAGAATTAATTAAGTATAATCAAACAGGTATGACATTAAACCAA
    GTTGCTCAAATACAAGCTGGTTTACAATATAAACCACAAGTACAACGTGTACCAGGTAAG
    TGGACAGATGCTAACTTTAATGATGTTAAGCATGCAATGGATACGAAGCGTTTAGCTCAA
    GATCCAGCATTAAAATATCAATTCTTACGCTTAGACCAACCACAAAATATTTCTATTGAT
    AAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTAGAAAACCAAGGTGCTGCATTTAAC
    AAAGCTGCTCAAATGTATGGCATTAATGAAGTTTATCTTATCTCACATGCCCTATTAGAA
    ACAGGTAACGGTACTTCTCAATTAGCGAAAGGTGCAGATGTAGTGAACAACAAAGTTGTA
    ACTAACTCAAACACGAAATACCATAACGTATTTGGTATTGCTGCATATGATAACGATCCT
    TTACGTGAAGGTATTAAATATGCTAAACAAGCTGGTTGGGACACAGTATCAAAAGCAATC
    GTTGGTGGTGCTAAATTCATCGGCAACTCATATGTAAAAGCTGGTCAAAATACACTTTAC
    AAAATGAGATGGAATCCTGCACATCCAGGAACACACCAATATGCTACAGATGTAGATTGG
    GCTAACATCAATGCTAAAATCATCAAAGGCTACTATGATAAAATTGGCGAAGTCGGCAAA
    TACTTCGACATCCCACAATATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3747

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS05135 [old locus tag: SA0905 ]
  • symbol: SA_RS05135
  • description: bifunctional autolysin
  • length: 1248
  • theoretical pI: 10.0803
  • theoretical MW: 136749
  • GRAVY: -0.612821

Function[edit | edit source]

  • reaction:
    EC 3.2.1.96?  ExPASy
    Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 9.9)
  • TheSEED: see SA0905
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 54.2)
    SH3 (CL0010) SH3_8; SH3-like domain (PF13457; HMM-score: 46.4)
    and 3 more
    no clan defined DGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 25.4)
    DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 13.5)
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 9.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.997209
    • TAT(Tat/SPI): 0.001289
    • LIPO(Sec/SPII): 0.001006
    • Cleavage Site: CS pos: 29-30. VQA-AE. Pr: 0.9829
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDSNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSEHQINPELIKSAAKPAALETQYKAAAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQFTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKSGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPIPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]