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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00345 [old locus tag: SA0040 ]
  • pan locus tag?: SAUPAN000104000
  • symbol: SA_RS00345
  • pan gene symbol?: mecI
  • synonym:
  • product: mecA-type methicillin resistance repressor MecI

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00345 [old locus tag: SA0040 ]
  • symbol: SA_RS00345
  • product: mecA-type methicillin resistance repressor MecI
  • replicon: chromosome
  • strand: +
  • coordinates: 48894..49265
  • length: 372
  • essential: no DEG

Accession numbers[edit | edit source]

  • Location: NC_002745 (48894..49265) NCBI
  • BioCyc: SA_RS00345 BioCyc
  • MicrobesOnline: see SA0040

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGATAATAAAACGTATGAAATATCATCTGCAGAATGGGAAGTTATGAATATCATTTGG
    ATGAAAAAATATGCAAGTGCGAATAATATAATAGAAGAAATACAAATGCAAAAGGACTGG
    AGTCCAAAAACCATTCGTACACTTATAACGAGATTGTATAAAAAGGGATTTATAGATCGT
    AAAAAAGACAATAAAATTTTTCAATATTACTCTCTTGTAGAAGAAAGTGATATAAAATAT
    AAAACATCTAAAAACTTTATCAATAAAGTATACAAAGGCGGTTTCAATTCACTTGTCTTA
    AACTTTGTAGAAAAAGAAGATCTATCACAAGATGAAATAGAAGAATTGAGAAATATATTG
    AATAAAAAATAA
    60
    120
    180
    240
    300
    360
    372

Protein[edit | edit source]

Protein Data Bank: 1OKR
Protein Data Bank: 1SAX
Protein Data Bank: 1SD6
Protein Data Bank: 1SD7
Protein Data Bank: 2D45

General[edit | edit source]

  • locus tag: SA_RS00345 [old locus tag: SA0040 ]
  • symbol: SA_RS00345
  • description: mecA-type methicillin resistance repressor MecI
  • length: 123
  • theoretical pI: 9.46589
  • theoretical MW: 14789.9
  • GRAVY: -0.74065

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 102.1)
    Signal transduction Regulatory functions DNA interactions copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 102.1)
    and 3 more
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 16.5)
    Signal transduction Regulatory functions DNA interactions phenylacetic acid degradation operon negative regulatory protein PaaX (TIGR02277; HMM-score: 16)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.8)
  • TheSEED: see SA0040
  • PFAM:
    HTH (CL0123) Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 130.6)
    and 10 more
    MarR; MarR family (PF01047; HMM-score: 23.4)
    PaaX; PaaX-like protein (PF07848; HMM-score: 17.9)
    TFIIE_alpha; TFIIE alpha subunit (PF02002; HMM-score: 17.2)
    no clan defined DUF3489; Protein of unknown function (DUF3489) (PF11994; HMM-score: 15.2)
    SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 14.7)
    HTH (CL0123) Cullin_Nedd8; Cullin protein neddylation domain (PF10557; HMM-score: 14.3)
    Sulfolobus_pRN; Sulfolobus plasmid regulatory protein (PF05584; HMM-score: 13.5)
    no clan defined DUF1160; Protein of unknown function (DUF1160) (PF06648; HMM-score: 13.2)
    post-AAA (CL0604) Rep_fac_C; Replication factor C C-terminal domain (PF08542; HMM-score: 12.7)
    HTH (CL0123) LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by MecI, TF important in Penicillin and methicillin resistance: see SA0040

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009467
    • TAT(Tat/SPI): 0.000301
    • LIPO(Sec/SPII): 0.001842
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDNKTYEISSAEWEVMNIIWMKKYASANNIIEEIQMQKDWSPKTIRTLITRLYKKGFIDRKKDNKIFQYYSLVEESDIKYKTSKNFINKVYKGGFNSLVLNFVEKEDLSQDEIEELRNILNKK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]