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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01359
  • pan locus tag?: SAUPAN003749000
  • symbol: SAOUHSC_01359
  • pan gene symbol?: mprF
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01359
  • symbol: SAOUHSC_01359
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1301484..1304006
  • length: 2523
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCAGGAAGTTAAAAACAAAATATTTTCAATCTTAAAAATTACGTTTGCTACAGCT
    TTATTTATTTTTGTAGCAATCACATTGTATCGGGAGTTATCTGGTATTAACTTTAAAGAT
    ACGTTGGTTGAATTTAGTAAGATTAACCGTATGTCCTTAGTGTTACTATTTATTGGTGGT
    GGGGCATCGCTTGTTATTCTATCAATGTATGATGTGATTTTATCTAGAGCTTTAAAAATG
    GATATATCCTTAGGCAAAGTTTTAAGAGTAAGTTATATCATCAATGCATTGAATGCGATT
    GTAGGTTTCGGTGGCTTTATTGGTGCAGGCGTTAGAGCAATGGTTTATAAAAACTATACG
    CATGATAAAAAGAAATTAGTTCACTTTATATCCTTAATACTTATTTCAATGTTGACAGGT
    TTAAGCTTATTATCATTGCTAATTGTATTCCATGTTTTCGATGCATCTTTAATCTTAGAT
    AAGATTACATGGGTAAGATGGGTATTATATGTAGTGTCATTTTTCTTACCATTATTCATT
    ATTTATTCAATGGTTAGACCACCCGATAAAAACAATCGTTTTGTAGGATTGTACTGCACT
    TTAGTGTCGTGTGTTGAATGGTTAGCAGCTGCAGTTGTATTATATTTCTGTGGTGTAATT
    GTTGACGCTCATGTATCATTCATGTCCTTTATTGCAATATTTATCATTGCTGCATTATCA
    GGTTTAGTCAGCTTTATTCCTGGTGGTTTCGGCGCTTTCGATTTAGTTGTATTACTAGGA
    TTTAAAACTTTAGGTGTCCCTGAGGAAAAAGTATTATTAATGCTACTTCTATATCGTTTT
    GCGTACTATTTTGTACCGGTAATTATTGCATTAATTTTATCATCATTTGAATTTGGTACA
    TCAGCTAAGAAGTACATTGAGGGATCTAAATACTTTATTCCTGCTAAAGATGTTACGTCA
    TTTTTAATGTCTTATCAAAAGGATATTATTGCTAAAATTCCATCATTATCATTAGCAATT
    TTAGTATTCTTTACAAGTATGATCTTTTTTGTAAATAACTTAACGATTGTTTACGATGCT
    TTATATGATGGAAATCACTTAACGTATTATATTCTATTGGCAATTCATACTAGTGCTTGT
    TTATTACTTTTACTGAATGTAGTTGGTATTTATAAGCAAAGTAGACGTGCCATTATCTTT
    GCTATGATTTCAATTTTATTAATCACAGTGGCGACATTCTTCACTTACGCTTCATATATT
    TTAATAACATGGTTAGCTATTATTTTTGTTCTGCTTATTGTAGCTTTCCGTAGAGCACGT
    AGGTTGAAACGCCCAGTAAGAATGAGAAATATAGTTGCAATGCTTTTATTCAGTTTATTT
    ATTTTATATGTTAACCATATATTTATTGCTGGAACGTTATATGCATTAGATATTTATACG
    ATTGAAATGCATACATCTGTATTGCGCTATTACTTCTGGCTTACGATTTTAATCATCGCT
    ATCATCATAGGTATGATTGCATGGTTGTTTGATTATCAATTTAGCAAAGTACGTATTTCT
    TCTAAAATTGAAGATTGCGAGGAGATTATTAATCAGTACGGCGGTAATTATTTGAGTCAC
    TTGATATATAGTGGTGACAAGCAGTTTTTCACTAATGAAAATAAAACAGCATTTTTAATG
    TATCGTTATAAAGCAAGTTCATTAGTGGTTCTTGGAGATCCGTTAGGTGATGAAAATGCC
    TTTGATGAATTGTTAGAAGCATTCTATAATTACGCTGAGTATTTAGGCTATGATGTTATA
    TTCTATCAAGTTACAGATCAACACATGCCTTTATATCATAATTTCGGTAACCAATTTTTC
    AAATTAGGTGAAGAAGCAATTATTGATTTAACGCAATTTTCAACTTCAGGTAAAAAACGC
    CGTGGATTTAGAGCGACTTTAAATAAATTCGATGAACTTAATATTTCGTTCGAAATTATT
    GAACCACCGTTTTCAACTGAATTTATAAATGAACTTCAACATGTAAGTGATTTATGGCTA
    GATAATCGTCAGGAAATGCATTTCTCTGTTGGTGAATTTAATGAAGAATACTTATCTAAA
    GCGCCAATTGGTGTAATGCGAAATGAAGAAAATGAAGTAATTGCATTTTGTAGTTTAATG
    CCAACATACTTTAATGATGCCATTTCAGTCGATTTAATTAGATGGTTGCCAGAGTTAGAT
    TTACCATTAATGGATGGTCTATACTTGCATATGTTACTTTGGAGTAAAGAACAAGGTTAT
    ACAAAATTTAATATGGGTATGGCAACGTTATCGAACGTTGGTCAATTGCATTATTCATAT
    TTAAGAGAACGACTTGCAGGCCGTGTCTTTGAACATTTCAACGGTCTATATCGTTTCCAA
    GGATTACGTCGTTATAAATCTAAATATAATCCGAATTGGGAACCACGCTTTTTAGTTTAT
    CGTAAAGATAATTCGCTTTGGGAATCACTTTCTAAAGTAATGCGTGTAATACGTCACAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2523

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01359
  • symbol: SAOUHSC_01359
  • description: hypothetical protein
  • length: 840
  • theoretical pI: 8.9615
  • theoretical MW: 96865.6
  • GRAVY: 0.509167

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.2.3?  ExPASy
    Lysyltransferase L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol
  • TIGRFAM:
    Unknown function General TIGR00374 family protein (TIGR00374; HMM-score: 48.1)
  • TheSEED  :
    • FmtC (MrpF) protein involved in methicillin resistance
    • L-O-lysylphosphatidylglycerol synthase (EC 2.3.2.3)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  FmtC (MrpF) protein involved in methicillin resistance
  • PFAM:
    Acetyltrans (CL0257) DUF2156; Uncharacterised conserved protein (DUF2156) (PF09924; HMM-score: 263.5)
    and 1 more
    no clan defined LPG_synthase_TM; Lysylphosphatidylglycerol synthase TM region (PF03706; HMM-score: 48.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008846
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.004849
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVEFSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVGFGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILDKITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCGVIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLLLYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIPSLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYKQSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRNIVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAWLFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKASSLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGEEAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDNRQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELDLPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYRFQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: GraR* (activation) regulon
    GraR*(response regulator)important in cationic antimicrobial peptide (CAMP) resistance;  [4]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ Mélanie Falord, Ulrike Mäder, Aurélia Hiron, Michel Débarbouillé, Tarek Msadek
    Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways.
    PLoS One: 2011, 6(7);e21323
    [PubMed:21765893] [WorldCat.org] [DOI] (I p)
  5. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

A Peschel, R W Jack, M Otto, L V Collins, P Staubitz, G Nicholson, H Kalbacher, W F Nieuwenhuizen, G Jung, A Tarkowski, K P van Kessel, J A van Strijp
Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine.
J Exp Med: 2001, 193(9);1067-76
[PubMed:11342591] [WorldCat.org] [DOI] (P p)Sascha A Kristian, Manuela Dürr, Jos A G Van Strijp, Birgid Neumeister, Andreas Peschel
MprF-mediated lysinylation of phospholipids in Staphylococcus aureus leads to protection against oxygen-independent neutrophil killing.
Infect Immun: 2003, 71(1);546-9
[PubMed:12496209] [WorldCat.org] [DOI] (P p)Yusuke Oku, Kenji Kurokawa, Norikazu Ichihashi, Kazuhisa Sekimizu
Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol.
Microbiology (Reading): 2004, 150(Pt 1);45-51
[PubMed:14702396] [WorldCat.org] [DOI] (P p)Petra Staubitz, Heinz Neumann, Tanja Schneider, Imke Wiedemann, Andreas Peschel
MprF-mediated biosynthesis of lysylphosphatidylglycerol, an important determinant in staphylococcal defensin resistance.
FEMS Microbiol Lett: 2004, 231(1);67-71
[PubMed:14769468] [WorldCat.org] [DOI] (P p)

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