From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06735 [old locus tag: SACOL1322 ]
  • pan locus tag?: SAUPAN003615000
  • symbol: SACOL_RS06735
  • pan gene symbol?:
  • synonym:
  • product: alpha/beta hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS06735 [old locus tag: SACOL1322 ]
  • symbol: SACOL_RS06735
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1340532..1341446
  • length: 915
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1340532..1341446) NCBI
  • BioCyc: SACOL_RS06735 BioCyc
  • MicrobesOnline: see SACOL1322

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTGAAAAACAATTTAAATTAACTGTACAAGATAATACGAATATTGAAGTTAAAGTG
    AATTTTACAGATGTAGATTCAAAAGGAATTATTCATATATTTCATGGTATGGCTGAACAT
    ATGGAACGTTACGATAAATTAGCACATGCACTTTCAAAGCATGGCTTCGATGTGATACGT
    CATAATCATCGAGGACATGGTATTAATATTGATGAATCAACAAGAGGGCATTACGATGAT
    ATGAAACGAGTTATCGGTGATGCCTTTGAAGTAGCGCAAACAGTGAGAGGCAATGTTGAT
    AAACCATACATTATAATCGGACATTCAATGGGATCCGTTATAGCTAGATTGTTTGTAGAA
    ACATATCCGCAATATGTTGATGGTCTAATTTTAAGTGGTACTGGTATGTATTCATTATGG
    AAAGGTTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAA
    CGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTA
    CGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAT
    CCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTA
    CATACATCACAATTAAAAAATATGAAAATGTTAAATCATGCCATGCCTATATTATTAGTT
    TCAGGATATGACGATCCTTTAGGTGATTATGGTAAAGGGATTTTAAAATTGGCGAATATA
    TATAGAAAAGCTGGCATTAAAAATGTTAAAGTGAATCTTTATCATCATAAACGTCATGAA
    GTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAA
    TTTTATAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS06735 [old locus tag: SACOL1322 ]
  • symbol: SACOL_RS06735
  • description: alpha/beta hydrolase
  • length: 304
  • theoretical pI: 9.65305
  • theoretical MW: 35255.3
  • GRAVY: -0.419408

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 35.4)
    and 5 more
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 25.2)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 24.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 23.3)
    proline-specific peptidase (TIGR01250; HMM-score: 22.9)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 14.3)
  • TheSEED: see SACOL1322
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 170.4)
    and 12 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 72.4)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 29.3)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 18.7)
    Peptidase_S28; Serine carboxypeptidase S28 (PF05577; HMM-score: 17.7)
    LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 17.4)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.5)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 16.2)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 15.9)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 15.8)
    Acyl_transf_2; Acyl transferase (PF02273; HMM-score: 15)
    DUF2920; Protein of unknown function (DUF2920) (PF11144; HMM-score: 15)
    LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 14.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004024
    • TAT(Tat/SPI): 0.00026
    • LIPO(Sec/SPII): 0.000268
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERYDKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKPYIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEKRVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYYMLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHHKRHEVLFEKDHDKIWEDLFKWLNQFYKK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1322
  • protein localization: see SACOL1322
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]