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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0177 [new locus tag: SACOL_RS00900 ]
  • pan locus tag?: SAUPAN001034000
  • symbol: murQ
  • pan gene symbol?: murQ
  • synonym:
  • product: N-acetylmuramic acid-6-phosphate etherase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0177 [new locus tag: SACOL_RS00900 ]
  • symbol: murQ
  • product: N-acetylmuramic acid-6-phosphate etherase
  • replicon: chromosome
  • strand: +
  • coordinates: 204259..205158
  • length: 900
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATG
    ACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTT
    CGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAG
    GGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCA
    GCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGTATTATTGCTGGT
    GGACAACATGCTATGACGATGGCTGTAGAAGGTGCGGAAGATCACAAAAAATTAGCGGAA
    GAAGATTTGAAAAATATAGATTTAACATCAAAAGATGTCGTTATAGGAATTGCCGCGAGT
    GGCAAAACGCCATATGTTATAGGCGGTTTAACATTTGCTAACACAATCGGTGCTACAACA
    GTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAA
    GTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAA
    AAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGAT
    AACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATT
    ATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAG
    CATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACA
    AGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0177 [new locus tag: SACOL_RS00900 ]
  • symbol: MurQ
  • description: N-acetylmuramic acid-6-phosphate etherase
  • length: 299
  • theoretical pI: 6.25943
  • theoretical MW: 32382.3
  • GRAVY: -0.0464883

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.2.1.126?  ExPASy
    N-acetylmuramic acid 6-phosphate etherase (R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H2O
  • ⊞TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramic acid 6-phosphate etherase (TIGR00274; EC 4.2.1.126; HMM-score: 352.6)
    and 5 more
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 25.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides phosphoheptose isomerase (TIGR00441; EC 5.3.1.28; HMM-score: 21.3)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 20.3)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 20.3)
    Unknown function General alpha-NAC homolog (TIGR00264; HMM-score: 14.2)
  • ⊞TheSEED  :
    • N-acetylmuramic acid 6-phosphate etherase (EC 4.2.1.126)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Sugars  N-acetylmuramic acid 6-phosphate etherase
    and 3 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Sugars  N-acetylmuramic acid 6-phosphate etherase (EC 4.2.1.126)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylmuramic acid 6-phosphate etherase
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylmuramic acid 6-phosphate etherase (EC 4.2.1.126)
  • ⊞PFAM:
    SIS (CL0067) GKRP_SIS_N; Glucokinase regulatory protein N-terminal SIS domain (PF22645; HMM-score: 75.4)
    and 8 more
    UBA (CL0214) GKRP-like_C; C-terminal lid domain of glucokinase regulatory protein (PF20741; HMM-score: 29.7)
    SIS (CL0067) GKRP_SIS_2; Glucokinase regulatory protein, second SIS domain (PF22198; HMM-score: 29.1)
    SIS_2; SIS domain (PF13580; HMM-score: 23.7)
    SIS; SIS domain (PF01380; HMM-score: 23.1)
    Beta_propeller (CL0186) RCC1; Regulator of chromosome condensation (RCC1) repeat (PF00415; HMM-score: 14.7)
    UBA (CL0214) UBA_HYPK; HYPK UBA domain (PF19026; HMM-score: 14)
    HTH (CL0123) HTH_PafC; PafC helix-turn-helix domain (PF19187; HMM-score: 12.3)
    DIP2311-like_C; Transcriptional regulator DIP2311-like, C-terminal domain (PF22168; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9993
    • Cytoplasmic Membrane Score: 0.0005
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003361
    • TAT(Tat/SPI): 0.001002
    • LIPO(Sec/SPII): 0.000945
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIPQLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQHAMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTVSISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVYDNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKEEATRRLLNNGDIVKRAIRDRQP

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulators: MurR* (repression) regulon, CcpA regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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