⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2453 [new locus tag: SA_RS14050 ]
pan locus tag^?: SAUPAN006405000
symbol: SA2453
pan gene symbol^?: msrA3
• synonym:
• product: methionine sulfoxide reductase A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2453 [new locus tag: SA_RS14050 ]
• symbol: SA2453
• product: methionine sulfoxide reductase A
• replicon: chromosome
• strand: -
• coordinates: 2765575..2766051
• length: 477
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125382 NCBI
• RefSeq: NP_375779 NCBI
• BioCyc: see SA_RS14050
• MicrobesOnline: 104805 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGTAGTTGTTTATGTAGCAGGAGGGTGTCTATGGGGTGTTGAAGCATTTTTTGCAACA
  ATACCTGGAATTATACATACAGAAGCAGGAAGAGCAAATGGAAGAAGCTCTAAATTAGAC
  GGTCCGTATGATGGTTATGCTGAATGTGTCAAACTTCATTTCGATGATCGTATGTTAACA
  ATTACAGACATTATGAATTATTTATTTGAAATCATTGATCCTTACAGTGTGAATCAACAA
  GGAAATGATATTGGAGAAAAGTATCGAACGGGTTTATATAGTTGTGTAGATGACCACTTA
  ATTGAAGCGCGTCAGTTCATTGAACGACGTAAAGACAGAGATCAAATTGCAGTGGAAGTC
  TTACCGCTGTCTAACTACATTAAAAGTGCTGAAGAACATCAGCAACATTTAGAAAAATAT
  CCAGAAGATATGCATATGTGTCATATTTCAAAAGATTTGCTAAATAAATACAAGTGA
  60
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  477
  

⊟Protein

⊟General

• locus tag: SA2453 [new locus tag: SA_RS14050 ]
• symbol: SA2453
• description: methionine sulfoxide reductase A
• length: 158
• theoretical pI: 5.24881
• theoretical MW: 18190.5
• GRAVY: -0.396203

⊟Function

• ⊞⊞reaction:
  EC 1.8.4.11?  ExPASy
  Peptide-methionine (S)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (S)-S-oxide + thioredoxin L-methionine + thioredoxin disulfide + H₂O = L-methionine (S)-S-oxide + thioredoxin
• TIGRFAM:
  Cellular processes Cellular processes Adaptations to atypical conditions peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 97.9)
  Genetic information processing Protein fate Protein modification and repair peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 97.9)
• TheSEED  :
  • Peptide-methionine (S)-S-oxide reductase MsrA (EC 1.8.4.11)
  Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)
• PFAM:
  no clan defined PMSR; Peptide methionine sulfoxide reductase (PF01625; HMM-score: 119.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9689
  • Cytoplasmic Membrane Score: 0.0051
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0255
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.395995
  • TAT(Tat/SPI): 0.013557
  • LIPO(Sec/SPII): 0.014432
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15928246 NCBI
• RefSeq: NP_375779 NCBI
• UniProt: A0A0H3JPE5 UniProt

⊟Protein sequence

• MVVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYDGYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIEARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2453 < SA2454

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Seoung Min Bong, Geun-Hee Kwak, Jin Ho Moon, Ki Seog Lee, Hong Seok Kim, Hwa-Young Kim, Young Min Chi
Structural and kinetic analysis of free methionine-R-sulfoxide reductase from Staphylococcus aureus: conformational changes during catalysis and implications for the catalytic and inhibitory mechanisms.
J Biol Chem: 2010, 285(32);25044-52
[PubMed:20504774] [WorldCat.org] [DOI] (I p)