PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0569 [new locus tag: SA_RS03290 ]
pan locus tag^?: SAUPAN002479000
symbol: SA0569
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0569 [new locus tag: SA_RS03290 ]
• symbol: SA0569
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 662818..663618
• length: 801
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123375 NCBI
• RefSeq: NP_373823 NCBI
• BioCyc: see SA_RS03290
• MicrobesOnline: 102849 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGATCTATTTACTAGAAAAGATGGAACATCGATACATTACAGTACATTAGGTGAAGGC
  TATCCTATCGTATTGATTCATACTGTACTTGATAATTATTCTGTGTTTAATAAATTAGCA
  GCACAATTAGCAAAATCATTTCAAGTTGTGTTAATTGATTTACGTGGACATGGCTATTCT
  GATAAACCTCGTCACATTGAAATAAAAGATTTTTCTGATGACATTGTTGAATTACTTAAA
  TATTTATACATTGAAGAAGTTGCATTTGTATGCCATGAAATGGGTGGAATCATTGGTGCG
  GATATTTCAGTACGTTATCCTGAATTTACATCATCACTTATGTTGGTAAATCCAACATCT
  ATTGAAGGTGAATTACCGGAAGAACGTTTATTTAGAAAATATGCCCATATTATTCGAAAC
  TGGGATCCTGAAAAACAAGATAAATTTTTAAATAAGCGTAAGTATTATCGTCCGAGAAAA
  ATGAATCGATTCCTTAAACATGTCGTAGATACAAATGAAATATCAACTAAAGAAGAAATT
  CAAGCAGTTAAAGAGGTATTCAAAAACGCTGATATTTCTCAAACTTATAGAAATGTCGTA
  GTACCGACAAAAATTATTGCAGGAGAATTCGGTGAAAGAACAACAAGATTGGAAGCTAAA
  GAAGTAGCTGATTTAATCCAAAATGCGGACTTTGAAGTATATCAAGAATCAAGTGCATTC
  CCATTTGTTGAAGAGCAAGAAAGATTCGTCGAAGATATAGCTTCATTTATCAACAAACAT
  CACGATGAAAAGCATGTTTAA
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⊟Protein

⊟General

• locus tag: SA0569 [new locus tag: SA_RS03290 ]
• symbol: SA0569
• description: hypothetical protein
• length: 266
• theoretical pI: 5.81589
• theoretical MW: 30982
• GRAVY: -0.413534

⊟Function

• ⊞TIGRFAM:
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 84.5)
  and 7 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 59.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 54.1)
  proline-specific peptidase (TIGR01250; HMM-score: 53.8)
  pyrimidine utilization protein D (TIGR03611; HMM-score: 42.9)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 33.6)
  2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 22.1)
  Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 12.6)
• TheSEED  :
  • Hydrolase, alpha/beta fold family
• ⊞PFAM:
  AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 64.4)
  Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 51.7)
  and 7 more
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 37.4)
  no clan defined PX; PX domain (PF00787; HMM-score: 18.6)
  AB_hydrolase (CL0028) Ndr; Ndr family (PF03096; HMM-score: 13.7)
  TPR (CL0020) Nipped-B_C; Sister chromatid cohesion C-terminus (PF12830; HMM-score: 13.5)
  TetR_C (CL0174) TetR_C_17; Tetracyclin repressor-like, C-terminal domain (PF17922; HMM-score: 13.5)
  PDDEXK (CL0236) RE_HindIII; HindIII restriction endonuclease (PF09518; HMM-score: 13.4)
  PUP (CL0408) POC1; POC1 chaperone (PF10450; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.89
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7611
  • Cytoplasmic Membrane Score: 0.2032
  • Cell wall & surface Score: 0.0036
  • Extracellular Score: 0.0321
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003056
  • TAT(Tat/SPI): 0.000289
  • LIPO(Sec/SPII): 0.000395
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926290 NCBI
• RefSeq: NP_373823 NCBI
• UniProt: A0A0H3JL06 UniProt

⊟Protein sequence

• MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLAKSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADISVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRKMNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLEAKEVADLIQNADFEVYQESSAFPFVEEQERFVEDIASFINKHHDEKHV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0566 > SA0567 > SA0568 > SA0569 > SA0570 > SA0571

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications