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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0100 [new locus tag: SA_RS00660 ]
  • pan locus tag?: SAUPAN000899000
  • symbol: SA0100
  • pan gene symbol?: nptA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0100 [new locus tag: SA_RS00660 ]
  • symbol: SA0100
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 113486..115153
  • length: 1668
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAATGTCGGTTACAGAAGTCATTTTCTCCTTTTTAGGTGGTTTAGGTATTTTCCTT
    TACGGCTTAAAAATCATGGGAGACGGGCTTCAAGCATCAGCAGGAGACAGGCTACGAGAT
    ATTTTAAACAAATTTACATCAAATCCAGTATTAGGTGTTATTGCAGGTATCGTTGTAACT
    ATTTTAATACAAAGTAGTTCAGGTACGACCGTTATCACAATCGGACTGGTAACAGCTGGA
    TTTATGACGTTAAAACAAGCTATTGGAGTGATAATGGGTGCCAATATCGGAACAACGGTA
    ACTGCATTTATTATCGGTATAGATTTAGGCGAATATGCAATGCCAATTTTAGCATTAGGT
    GCATTTTTAATCTTTTTCTTTAAACGCTCTAAAATCAATAACATTGGCCGCATACTATTC
    GGTTTCGGTTCTCTATTCTTCGGTCTAGAATTTATGGGTGATGCCGTTAAACCTTTAGCA
    TCATTAGATGGATTTAAGCAATTAATGCTTGATATGTCATCAAATCCAATACTTGCTGTC
    ATTGTCGGCGCAGGGTTAACAGCACTAGTTCAAAGTTCAAGTGCGACGATTGGTATTTTA
    CAAGAATTTTATCAACAAGATTTAATCAGCTTAAACGCAGCAATCCCTGTGTTACTAGGT
    GATAACATTGGTACAACAATTACAGCTATCTTAGCTAGTTTAGCCGGCTCAATCGCTGCA
    AAACGTGCGGCGCTTGTACACGTCATCTTTAATTTAATCGGGGTAATTATCTTCACAATT
    TTCTTGCCAGTTGTGATTCATTTGATTAGTTTGTTACAAGATGTATGGCACTTAAAACCA
    GCGATGACGATTGCAGTATCACATGGTATCTTCAACATAACAAATACTTTGATTCAATTA
    CCATTTGTAGCAGGTTTAGCATGGATAGTTACAAAGCTTGTCCCAGGTAAAGATATTGCT
    GATGACTATAAACCTCAGCACTTAAACAAAGATCTTGTTTATCACGCACCTGGTGTTGCA
    TTACAAGAAACTCAAAAAGAATTACAAAATGTGGGTCAGATTGTCTTATCAATGTTTGAA
    GACATTCGCGAAATTACAAAAGACGATAAAAAATTGATCAAGAAGCTTGAACAAAAGCAC
    CAAGCTGTTGAAACAATCAATGATAGCATTCGAAATTATTTAGTTAGAATTTCTACAAAA
    GCCATTACGAAGGCAGACGTTGAGCGTTTAGCAGTTATGTTTGATGTCAATCGCTCTATT
    TTAAAAGTAGCAGAGCTAACAGAAGAGTATGTCGCTCAATTAAAACGCCAACATGATGAA
    GATATTCGCATTACAGAAGATGCACAACGCGGTATGGATAAATTATTCAACCATGTTGCT
    GAGTCATTTGATAAAGCCATCGACATGTTAGATGTTTATGACAAAACGAAAAAAGATGAA
    ATTGTAGAACGTAGTAGAGAATCATTTAATATTGAACATAAACTACGCAAAGGTCATATT
    AAACGCCTTAATCGTGGCGAATGTACAACAAAAGGCGGATTACTATATATCGATATGATT
    GGTGTTCTTGAACGTATCGGTTATCATTCACGAAATGTTTCTGAAGCACTTGTTGGTCTT
    AACGATGATGTACCTACAGATGAAGAAATTGCAACAACTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0100 [new locus tag: SA_RS00660 ]
  • symbol: SA0100
  • description: hypothetical protein
  • length: 555
  • theoretical pI: 6.25194
  • theoretical MW: 60879.7
  • GRAVY: 0.32991

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds Na/Pi-cotransporter II-related protein (TIGR00704; HMM-score: 250)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds sodium-dependent inorganic phosphate (Pi) transporter (TIGR01013; HMM-score: 120.8)
    Metabolism Transport and binding proteins Anions phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
    Signal transduction Regulatory functions Other phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
  • TheSEED  :
    • Na/Pi cotransporter II-related protein
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Na/Pi cotransporter II-related protein
  • ⊞PFAM:
    no clan defined Na_Pi_cotrans; Na+/Pi-cotransporter (PF02690; HMM-score: 232.6)
    and 7 more
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 58.6)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 12.7)
    no clan defined ApoO; Apolipoprotein O (PF09769; HMM-score: 12.5)
    IBP39; Initiator binding protein 39 kDa (PF11422; HMM-score: 12.4)
    FAD-oxidase_C (CL0277) Cytokin-bind; Cytokinin dehydrogenase 1, FAD and cytokinin binding (PF09265; HMM-score: 12)
    Phage_TACs (CL0567) Phage_TAC_19; Phage tail chaperone protein (PF23857; HMM-score: 11.4)
    NTF2 (CL0051) DUF7309; Domain of unknown function (DUF7309) (PF23988; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9994
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0004
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.075555
    • TAT(Tat/SPI): 0.006562
    • LIPO(Sec/SPII): 0.006645
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEMSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFTSNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFIIGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASLDGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLGDNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHLKPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHAPGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYLVRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGMDKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTTKGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)

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