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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0547 [new locus tag: NWMN_RS03140 ]
  • pan locus tag?: SAUPAN002362000
  • symbol: NWMN_0547
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0547 [new locus tag: NWMN_RS03140 ]
  • symbol: NWMN_0547
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 630314..631798
  • length: 1485
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAAAAAAGAATAAGCAAATAGATAGAGGCGATTTAAAACAAAACCTATCTGAAAAG
    TTTGTATGGGCGATTGCATATGGTTCATGTATCGGATGGGGCGCATTCATCTTACCAGGA
    GACTGGATTAAGCAGTCAGGTCCGATTGCAGCATCAATTGGTATAGTTATTGGTGCATTA
    TTAATGATATTAATTGCGGTTAGTTATGGCGCATTAGTAGAGAGATTTCCAGTATCAGGG
    GGCGCGTTTGCCTTTAGTTTCTTAAGTTTCGGCAGATATGTGAGTTTCTTCTCATCATGG
    TTTTTAACTTTTGGTTATGTCTGTGTCGTTGCTTTAAATGCGACCGCATTCAGTTTACTA
    GTTAAATTCTTATTGCCAGATGTCTTAAATAATGGGAAACTATACACCATTGCGGGCTGG
    GACGTTTATATTACGGAAATCATTATTGCGACCGTATTACTACTTGTATTCATGCTAGTA
    ACGATTCGTGGCGCAAGTGTATCTGGATCATTACAATATTATTTCTGTGTGGCGATGGTA
    ATCGTCGTATTATTGATGTTCTTTGGTTCATTCTTTGGTAATAATTTTGCACTTGAAAAT
    TTACAACCGTTAGCTGAACCTAGCAAAGGATGGTTAGTGTCTATTGTGGTTATTGTATCC
    GTGGCACCATGGGCATATGTTGGATTTGATAATATTCCACAAACAGCAGAAGAGTTTAAC
    TTTGCACCAAACAAGACATTTAAGCTTATCGTGTACAGTTTATTAGCAGCATCATTAACT
    TATGTTGTCATGATTTTATACACTGGTTGGTTATCAACAAGTCATCAAAGTTTAAATGGG
    CAGTTGTGGTTAACAGGTGCTGTTACACAAACAGCATTTGGTTATATTGGATTAGGTGTA
    TTAGCAATTGCAATTATGATGGGTATATTTACTGGTTTAAATGGATTCTTGATGAGTTCA
    AGTCGCTTGTTATTTTCTATGGGACGTTCAGGTATTATGCCAACAATGTTTAGTAAATTA
    CATAGTAAATACAAAACACCATATGTCGCAATCATATTCCTAGTAGGAGTGTCGTTAATT
    GCACCTTGGCTAGGAAGAACTGCATTGACTTGGATTGTAGATATGTCATCTACTGGTGTA
    TCCATTGCCTACTTTATTACATGTTTGTCTGCAGCGAAATTATTCAGTTATAACAAACAA
    AGTAATACGTATGCACCGGTTTACAAAACGTTTGCTATTATCGGCTCATTTGTATCATTC
    ATTTTCTTAGCGTTGTTATTAGTGCCAGGTTCTCCTGCAGCACTGACTGCACCGTCTTAT
    ATTGCATTACTTGGATGGTTAATCATCGGTTTAATATTCTTTGTGATTCGATATCCTAAA
    TTGAAAAATATGGATAATGATGAATTAAGTCGCTTGATTTTAAATAGAAGTGAAAATGAA
    GTTGATGATATGATTGAAGAACCTGAAAAAGAAAAAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0547 [new locus tag: NWMN_RS03140 ]
  • symbol: NWMN_0547
  • description: hypothetical protein
  • length: 494
  • theoretical pI: 9.05617
  • theoretical MW: 54336.8
  • GRAVY: 0.731579

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 129)
    and 8 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 75.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 65.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 56.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 48.7)
    permease, urea carboxylase system (TIGR03428; HMM-score: 47.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 35.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 29.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 23.2)
  • TheSEED  :
    • cationic amino acid transporter
  • ⊞PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 129.6)
    and 2 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 75)
    no clan defined DUF2615; Protein of unknown function (DUF2615) (PF11027; HMM-score: 10)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9998
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02156
    • TAT(Tat/SPI): 0.000941
    • LIPO(Sec/SPII): 0.086079
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIKQSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFLTFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLVTIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVIVSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQSLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMPTMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAAKLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIGLIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SigB (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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