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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11930 [old locus tag: SACOL2269 ]
  • pan locus tag?: SAUPAN005737000
  • symbol: SACOL_RS11930
  • pan gene symbol?: moeB
  • synonym:
  • product: molybdopterin biosynthesis protein MoeB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS11930 [old locus tag: SACOL2269 ]
  • symbol: SACOL_RS11930
  • product: molybdopterin biosynthesis protein MoeB
  • replicon: chromosome
  • strand: -
  • coordinates: 2330575..2331579
  • length: 1005
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2330575..2331579) NCBI
  • BioCyc: SACOL_RS11930 BioCyc
  • MicrobesOnline: see SACOL2269

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAGTCAAGAACGTTATTCAAGGCAAATTTTATTTAAACAAATAGGTGAAATAGGTCAA
    AGCAAAATAAATCAAAAATGTGCGTTGATTATTGGTATGGGCGCATTAGGTACACATGTG
    GCCGAAGGACTTGTTAGAGCAGGCATTGCCAAACTAATCATTGTTGATAGAGATTATATT
    GAATTTAGTAATTTACAAAGACAAACATTGTTTACTGAAGAAGATGCTTTGAAAATGATG
    CCTAAGGTGGTTGCAGCTAAAAAGCATTTGCTAGCGTTACGTAGTGATGTTGATATTGAT
    GATTATATTGCCCATGTGGATTATTATTTTTTGGAAACACATGGACAGGACGTTGACGTT
    ATTATTGATGCAACCGATAACTTTGAAACACGACAACTGATTAATGATTTTGCATATAAA
    TATCGTATACCTTGGATTTATGGTGGTGTTGTACAGAGTACATATACAGAAGCTGCATTT
    ATACCTGGTAAAACACCTTGCTTTAACTGTTTGGTACCACAATTGCCAGCATTAAATTTA
    ACATGTGATACAGTAGGGGTCATTCAACCTGCCGTGACGATGGCAACAAGTTTACAATTA
    AGAGATGCGATGAAAGTATTAACGGAACAACCAATTGACACAAAAATAACTTATGGCGAT
    ATTTGGGAAGGTAGTCATTATTCATTTGGTTTCAGTAAAATGCAACGTTCAGACTGTACA
    ACTTGTGGAGATGTACCAAGTTATCCGTATTTAAACAAGAATGAACAACGTTATGCAACA
    TTGTGTGGTAGAGACACTGTACAGTATGAAAATGCATCAATTACACACGACATTCTTGTT
    CAATTTTTAAAACAACATCAGTTAAATTATCGCAGTAATTCGTATATGGTTATGTTTGAA
    TTTAAAGGACACCGCATTGTTGCTTTTAAAGGTGGAAGGTTTTTAATACATGGCATGACA
    CGCACATCAGATGCCACACATCTAATGAATTTATTGTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS11930 [old locus tag: SACOL2269 ]
  • symbol: SACOL_RS11930
  • description: molybdopterin biosynthesis protein MoeB
  • length: 334
  • theoretical pI: 6.84183
  • theoretical MW: 37920.3
  • GRAVY: -0.14491

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 204.8)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 124.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 67)
    ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 37.1)
    E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 32.8)
    Cellular processes Cellular processes Pathogenesis thiazole/oxazole-forming peptide maturase, SagC family component (TIGR03603; EC 6.3.-.-; HMM-score: 32.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 21.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.1)
    PRTRC system ThiF family protein (TIGR03736; HMM-score: 11.2)
  • TheSEED: see SACOL2269
  • ⊞PFAM:
    NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 190.7)
    and 9 more
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 24.3)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 17)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.8)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15.7)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.2)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.5)
    KR; KR domain (PF08659; HMM-score: 13.8)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12.3)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9577
    • Cytoplasmic Membrane Score: 0.0404
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0013
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.066023
    • TAT(Tat/SPI): 0.024209
    • LIPO(Sec/SPII): 0.002925
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 445999074 NCBI
  • RefSeq: WP_000076929 NCBI
  • UniProt: see SACOL2269

⊟Protein sequence[edit | edit source]

  • MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGLVRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDYIAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAFIPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITYGDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITHDILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2269
  • protein localization: see SACOL2269
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS11930&oldid=101288"
  • This page was last edited on 11 March 2016, at 13:29.
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