⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS09650 [old locus tag: SACOL1880 ]
pan locus tag^?: SAUPAN004583000
symbol: SACOL_RS09650
pan gene symbol^?: lukD
• synonym:
• product: leucotoxin LukDv

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS09650 [old locus tag: SACOL1880 ]
• symbol: SACOL_RS09650
• product: leucotoxin LukDv
• replicon: chromosome
• strand: -
• coordinates: 1934671..1935654
• length: 984
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1934671..1935654) NCBI
• BioCyc: SACOL_RS09650 BioCyc
• MicrobesOnline: see SACOL1880

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAATGAAAAAATTAGTCAAATCATCAGTTGCTTCATCAATTGCACTGCTTTTGCTA
  TCGAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGCGAGAAAAAAGTAGATGAC
  AAAATCACTTTATACAAAACAACAGCAACATCTGATAATGATAAATTGAATATTTCTCAA
  ATTTTAACGTTTAATTTCATTAAGGATAAAAGTTATGACAAAGATACGTTAGTACTTAAG
  GCAGCCGGAAACATTAATTCAGGTTATAAAAAGCCTAATCCAAAAGATTACAATTACTCA
  CAGTTTTATTGGGGCGGTAAGTATAATGTTTCGGTTAGTTCAGAATCAAATGATGCTGTA
  AATGTTGTTGACTATGCACCTAAAAATCAAAATGAAGAATTCCAAGTTCAACAAACATTA
  GGTTATTCTTATGGCGGAGATATTAATATATCTAATGGCTTATCAGGTGGATTAAATGGA
  TCAAAATCATTTTCAGAAACGATAAATTATAAACAAGAAAGTTACAGAACTACGATTGAT
  AGAAAAACAAATCATAAATCAATTGGCTGGGGTGTTGAGGCGCACAAAATTATGAATAAT
  GGTTGGGGACCATATGGTAGAGATAGTTATGACCCAACATATGGTAATGAACTGTTTTTA
  GGCGGTAGACAAAGTAGTTCAAATGCTGGTCAAAATTTCTTGCCAACACATCAAATGCCT
  TTATTGGCGCGTGGTAACTTTAACCCAGAATTTATAAGCGTACTTTCTCATAAACAAAAT
  GATACAAAAAAATCTAAAATCAAAGTAACTTACCAAAGAGAAATGGATAGATATACTAAT
  CAATGGAATCGACTACACTGGGTTGGTAATAACTACAAAAATCAAAATACAGTAACGTTT
  ACATCTACTTATGAAGTTGACTGGCAAAACCATACTGTTAAATTAATCGGTACGGATTCT
  AAAGAAACTAATCCTGGAGTATAA
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⊟Protein

• 4Q7G
  PDB

⊟General

• locus tag: SACOL_RS09650 [old locus tag: SACOL1880 ]
• symbol: SACOL_RS09650
• description: leucotoxin LukDv
• length: 327
• theoretical pI: 9.65379
• theoretical MW: 36888.7
• GRAVY: -0.815596

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 441.5)
• TheSEED: see SACOL1880
• PFAM:
  Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 330.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.9998
• LocateP:
• ⊞⊞SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.995468
  • TAT(Tat/SPI): 0.000385
  • LIPO(Sec/SPII): 0.003085
  • Cleavage Site: CS pos: 26-27. VDA-AQ. Pr: 0.9634
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446705118 NCBI
• RefSeq: WP_000782464 NCBI
• UniProt: see SACOL1880

⊟Protein sequence

• MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV

⊟Experimental data

• experimentally validated: see SACOL1880
• protein localization: see SACOL1880
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SACOL1880

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications