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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0129 [new locus tag: NWMN_RS00710 ]
  • pan locus tag?: SAUPAN001025000
  • symbol: argD
  • pan gene symbol?: argD
  • synonym:
  • product: ornithine aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0129 [new locus tag: NWMN_RS00710 ]
  • symbol: argD
  • product: ornithine aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 161034..162218
  • length: 1185
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATTCAATCATTGAATTAACTGATTATTATAGCTCTAATAATTATGCACCACTTAAG
    CTTGTCATTTCTAAAGGTAAAGGTGTCAAAGTTTGGGATACTGATGGCAAACAATATATA
    GATTGCATTTCGGGTTTTTCAGTTGCAAACCAAGGCCATTGTCATCCAACAATTGTTAAA
    GCGATGACAGAACAAGCTTCAAAGTTGTCTATCATTTCACGTGTCCTTTATAGTGACAAT
    CTCGGGAAATGGGAAGAAAAAATTTGTCATCTTGCTAAGAAAGACAAAGTACTCCCCCTT
    AACTCTGGTACTGAAGCTGTTGAAGCAGCCATTAAAATTGCTAGAAAATGGGGCTCTGAA
    GTTAAAGGCATTACTGACGGACAAGTTGAAATCATCGCTATGAATAACAATTTTCACGGT
    CGTACACTTGGCTCATTATCACTATCTAACCACGACGCATATAAAGCAGGATTTCACCCC
    CTACTTCAAGGCACTACAACAGTAGATTTTGGAGACATTGAACAATTAACACAAGCTATT
    TCACCGAATACAGCAGCAATTATTTGGGAACCAATTCAAGGTGAAGGTGGCGTTAATATA
    CCACCGAAAGGATATATTCAAGCTGTGCGTCAACTATGTGATAAACATCAAATATTATTG
    ATTGCAGATGAAATTCAAGTTGGTCTTGGTAGAACTGGGAAATGGTTTGCTATGGAATGG
    GAGCAAGTCGTTCCAGACATTTATATTTTAGGTAAGGCATTGGGTGGCGGCTTATACCCT
    GTATCTGCTGTACTTGCAAATAATGATGTCATGCGTGTTCTAACACCAGGTACACATGGT
    TCAACATTTGGTGGTAACCCTTTAGCCATTGCAATATCGACGGCAGCGCTTGATGTACTT
    AAAGATGAACAACTGGTTGAACGATCAGAACGCTTAGGTTCATTTTTATTAAAAGCGTTG
    CTACAACTTAAACATCCTAGTATTAAAGAAATTAGAGGTCGTGGTTTATTTATAGGCATA
    GAGCTTAACACAGATGCTGCACCTTTTGTGGATCAACTGATTCAACGTGGAATCTTATGC
    AAAGACACGCATCGTACTATCATTCGATTGTCTCCACCTCTAGTCATTGATAAAGAGGAA
    ATCCATCAAATTGTTGCAGCTTTTCAAGACGTTTTTAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0129 [new locus tag: NWMN_RS00710 ]
  • symbol: ArgD
  • description: ornithine aminotransferase
  • length: 394
  • theoretical pI: 7.10134
  • theoretical MW: 43121.4
  • GRAVY: -0.04467

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.6.1.13?  ExPASy
    Ornithine aminotransferase L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid
  • ⊞TIGRFAM:
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 485.4)
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 422.2)
    and 13 more
    Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 326.2)
    Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 268.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 260.6)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 246.2)
    Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 213.8)
    Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 195.3)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 158.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 144.2)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 107.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 14)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 12.9)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 12.3)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 11.9)
  • TheSEED  :
    • Acetylornithine aminotransferase 1 (EC 2.6.1.11)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Acetylornithine aminotransferase 1 (EC 2.6.1.11)
  • ⊞PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 440.6)
    and 6 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 23.9)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 19)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 18.9)
    KYNU_C; Kynureninase C-terminal domain (PF22580; HMM-score: 13.8)
    HTH (CL0123) MRB1590_C; MRB1590 C-terminal domain (PF21117; HMM-score: 12.7)
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9776
    • Cytoplasmic Membrane Score: 0.007
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0149
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.132451
    • TAT(Tat/SPI): 0.000497
    • LIPO(Sec/SPII): 0.001728
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCISGFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNSGTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHPLLQGTTTVDFGDIEQLTQAISPNTAAIIWEPIQGEGGVNIPPKGYIQAVRQLCDKHQILLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTPGTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGRGLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVFKN

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: ArgR* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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