⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS13985 [old locus tag: SA2440 ]
pan locus tag^?: SAUPAN006380000
symbol: SA_RS13985
pan gene symbol^?: gtfB
• synonym:
• product: accessory Sec system glycosylation chaperone GtfB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS13985 [old locus tag: SA2440 ]
• symbol: SA_RS13985
• product: accessory Sec system glycosylation chaperone GtfB
• replicon: chromosome
• strand: -
• coordinates: 2744596..2745954
• length: 1359
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2744596..2745954) NCBI
• BioCyc: SA_RS13985 BioCyc
• MicrobesOnline: see SA2440

⊟Phenotype

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⊟DNA sequence

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  ATGATTAAATTATTTGATTACTTTAACGATCATTCACGAAAGTTATATGAGTCTTTTAAA
  GCCAGTAAGTTGGAAGAAGATTTAACAATTGTACTCAATGACAATGGCTTTTTACCGGAC
  GATGTTATATCACCGTATCAATTTTTTGCAGATAATCATAATACAGAAAATATGAAACCA
  CGATTTTTTAATCAGGTTACTGTACCAGCTTTTTGGGAAATTAAAGGTAACAACAATTCG
  GCTACAATTAATGATATGGGACGTCTACGAGGCAAAATATTTTATCAAAGTGGAGAGCGC
  CCTAGAATTGTTAGTCGTGTCGAATGGTTTGATGACCAACAACGTGTCCGATTTGTCGAT
  TATTATTCTAAGAATGGTATTAAATTTGCACAAACTGTTTACGATTTAAATCGTAAAGCG
  ATCTTGAAGAAATATATGACAGCAGAAGGTAAAGAAGTGATTTATGAAAATTTTGTAACA
  TCTGATGTCATATTGGATTGGCAAGGGAAGTCTTACTTTTTCCCTTCAAAGCTCGCATTC
  GTATTATTTTTTATCAAGCAACTTGAAATTACGGAACATCATTTCGTTATTAACTCACTA
  GCCCTACCATTTTCAGTGTTATATAATTTGCCATCAAAAGGTAGTGATGTTTTGGTATGG
  CAAGAACACTGTAATGGTAATGTCCCGGGAAATATGCAATTGATGTGTAAAGGTGATATG
  AAACGACATTGTAATATTATTATTCCAGATAAAAACGAATATGAAACGATGCTCAATATA
  GCAGACGCAAAGGTTCAATCTCGTATTTTACAAGGCGGATATCTATATAACTATCGTAGT
  CGTAATAGATATACTAAAGAAATTGTAATATTAACTAACTCGGATCAACTTCGTAACATC
  AAGGTATTGGTTGAAACTTTACCAGATTTTAATTTTCACATCGCTGCTATTACAGAGATG
  TCTGATAAGCTAATGCAACTTGATCAATATGCTAATGTGCACTTATACCCATCTATTAAT
  ATTGACAGGGTAAATGAACTATACCAGTTATGTGATATTTATCTTGATATCAATGAGGGT
  AATGAAATTTTAAATGCGGTGGAACAAGCGTTTGACTATGAGTTGTTGATTTTAGGTTAT
  CGTCAAACCGCACACCATGCTAAAGTAACATTATCAGAGCACTTATTTGAGCATAATGAT
  GAGATAACAATGGAAAGTAAAGACCAATTAATACAAATGTTAGAAAGTTTGAAAGATCAA
  CAACAGTTTCGAGATGCTTTATTAGCACAAAAAGCACATGCACACGAAATCTCGCGTGAA
  CAATTCGAGCAAGTTTTTAAACAGGCGCTCGAAAGCTAA
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⊟Protein

⊟General

• locus tag: SA_RS13985 [old locus tag: SA2440 ]
• symbol: SA_RS13985
• description: accessory Sec system glycosylation chaperone GtfB
• length: 452
• theoretical pI: 5.75028
• theoretical MW: 53041.9
• GRAVY: -0.410841

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 666.9)
• TheSEED: see SA2440
• PFAM:
  GT-B (CL0113) Glyco_trans_1_2; Glycosyl transferase-like (PF13524; HMM-score: 16.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9423
  • Cytoplasmic Membrane Score: 0.0119
  • Cell wall & surface Score: 0.0022
  • Extracellular Score: 0.0435
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001797
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000283
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446515810 NCBI
• RefSeq: WP_000593156 NCBI
• UniProt: see SA2440

⊟Protein sequence

• MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVISPYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPRIVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVTSDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVLVWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLYNYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFNFHIAAITEMSDKLMQLDQYANVHLYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLSEHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQALES

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications