⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1924 [new locus tag: NWMN_RS11105 ]
pan locus tag^?: SAUPAN005161000
symbol: NWMN_1924
pan gene symbol^?: hlb
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1924 [new locus tag: NWMN_RS11105 ]
• symbol: NWMN_1924
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2130661..2131125
• length: 465
• essential: unknown

⊟Accession numbers

• Gene ID: 5331188 NCBI
• RefSeq: YP_001332958 NCBI
• BioCyc:
• MicrobesOnline: 3707512 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  ATGAAAAGATTGTTAGGTTTATTATTAGTGAGCACGTTAGTGTTAAGTGCATGTGGAAAT
  GATGAGAATCAGGAAGAATCTAAAAAAGAAGTTAAATCAAAAGAAAAGAAAATTGAGAAG
  GAAAAGGAAAATAAATCGAAAAAAGATAAGGAAAAAGAAGTTGCAACACAACAACAACCA
  GACAATCAAACCGTTGAACAACCCCAATCACAAGAGCAATCGGTTCAACAACCGCAACAA
  CAGATACCACAAAATAGTGTTCCTCAGCAAAATGTCCAAGTTCAACAAAACAAAAAGCAA
  AAAGTTGATTTAAATAATATGCCTCCCACTGATTTTTCTACAGAGGGTATGTCTGAGCAG
  GCTCAAAAACAAATTGAAGAGCTTTCAATGCAAAAAGACTATCATGGTCTGTCACAAAGA
  GAATACAATGATAGAGTTTCTGAAATTATAAATAATGATAATTGA
  60
  120
  180
  240
  300
  360
  420
  465
  

⊟Protein

⊟General

• locus tag: NWMN_1924 [new locus tag: NWMN_RS11105 ]
• symbol: NWMN_1924
• description: hypothetical protein
• length: 154
• theoretical pI: 4.93322
• theoretical MW: 17766.5
• GRAVY: -1.44545

⊟Function

• ⊞⊞TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15.1)
  and 9 more
  Cellular processes Cellular processes Cell division cell division protein ZipA (TIGR02205; HMM-score: 9.4)
  cobaltochelatase subunit (TIGR02442; EC 6.6.1.2; HMM-score: 8.8)
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 8.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 7.1)
  Cellular processes Cellular processes Pathogenesis putative immunoglobulin-blocking virulence protein (TIGR04526; HMM-score: 6.5)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 4.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 3.4)
  Genetic information processing Transcription Degradation of RNA ribonuclease Y (TIGR03319; EC 3.1.-.-; HMM-score: 3)
  Metabolism Transport and binding proteins Other K-Cl cotransporter (TIGR00930; HMM-score: 2.5)
• TheSEED  :
  • Phage protein
• ⊞⊞PFAM:
  no clan defined LYRIC; Lysine-rich CEACAM1 co-isolated protein family (PF15686; HMM-score: 16.4)
  DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 13.4)
  Trypan_PARP; Procyclic acidic repetitive protein (PARP) (PF05887; HMM-score: 13.2)
  and 11 more
  ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 12.4)
  DUF4969; Domain of unknown function (DUF4969) (PF16339; HMM-score: 11.5)
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 11.1)
  no clan defined TP53IP5; Cellular tumour antigen p53-inducible 5 (PF15331; HMM-score: 10.8)
  Pinin_SDK_memA; pinin/SDK/memA/ protein conserved region (PF04696; HMM-score: 10.1)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 10.1)
  no clan defined DUF4887; Domain of unknown function (DUF4887) (PF16228; HMM-score: 9.3)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 8.6)
  no clan defined PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 7.6)
  AhpD-like (CL0423) PA26; PA26 p53-induced protein (sestrin) (PF04636; HMM-score: 6.9)
  FUSC (CL0307) ALMT; Aluminium activated malate transporter (PF11744; HMM-score: 6.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0089
  • Cytoplasmic Membrane Score: 0.6009
  • Cell wall & surface Score: 0.188
  • Extracellular Score: 0.2023
• ⊞⊞LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: VLSACGN
• ⊞⊞SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000386
  • TAT(Tat/SPI): 0.000086
  • LIPO(Sec/SPII): 0.999259
  • Cleavage Site: CS pos: 17-18. LSA-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222136 NCBI
• RefSeq: YP_001332958 NCBI
• UniProt: A0A0H3K9G6 UniProt

⊟Protein sequence

• MKRLLGLLLVSTLVLSACGNDENQEESKKEVKSKEKKIEKEKENKSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQIPQNSVPQQNVQVQQNKKQKVDLNNMPPTDFSTEGMSEQAQKQIEELSMQKDYHGLSQREYNDRVSEIINNDN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1924 > int > NWMN_1926

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications