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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08995 [old locus tag: SA1597 ]
  • pan locus tag?: SAUPAN004475000
  • symbol: SA_RS08995
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08995 [old locus tag: SA1597 ]
  • symbol: SA_RS08995
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1835083..1835526
  • length: 444
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1835083..1835526) NCBI
  • BioCyc: SA_RS08995 BioCyc
  • MicrobesOnline: see SA1597

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    301
    361
    421
    ATGAATAAAGCATCATTTGATAAAAAAGTTAAAAAGCAATTATGGTTTTTGAACAAGAAG
    GAGAAACAAGCATTAGATCAACGATTATCTTCTATATCTGATGATGACAGCGTTAACTTA
    AATAAGCCTGTTACCTTTGCTAATGCTTACTTAAGACAAAATGTATTTCGAAATAAAGAA
    ACAAAAAGTTATAGTATGTTTGTTACATTAGTAGTGATGATGTTTGCTTATGTTGCTTTA
    TTAGGCTTGTTTTTATTTGGGCTAATTACAAGTCTATCTGGCGTACAATTTTTCGTTAGT
    CCTAAGGTAGATTTATCAACAACTGTTGTTATTTTAACAATTATTGGAGCGATTTTATTA
    ATGATTGTTAGCATATATTTTATAAAAATTGTGACATCATACTTTACTAAGAAACTTTTA
    GAAATTAAATTTAATTCGAAATAG
    60
    120
    180
    240
    300
    360
    420
    444

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08995 [old locus tag: SA1597 ]
  • symbol: SA_RS08995
  • description: hypothetical protein
  • length: 147
  • theoretical pI: 10.5648
  • theoretical MW: 16798.9
  • GRAVY: 0.385714

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA1597
  • ⊞PFAM:
    Transporter (CL0375) Claudin_2; PMP-22/EMP/MP20/Claudin tight junction (PF13903; HMM-score: 20.1)
    no clan defined DUF2207_C; Predicted membrane protein (DUF2207) C-terminal domain (PF20990; HMM-score: 17.1)
    and 6 more
    MS_channel_1st_1; Mechanosensitive ion channel, conserved TM helix (PF05552; HMM-score: 15.5)
    TPR (CL0020) DUF6584; Family of unknown function (DUF6584) (PF20225; HMM-score: 13.4)
    no clan defined DUF3671; Fam-L, Fam-M like protein (PF12420; HMM-score: 13.1)
    DUF378; Domain of unknown function (DUF378) (PF04070; HMM-score: 12.3)
    DUF7248; Family of unknown function (DUF7248) (PF23906; HMM-score: 9.7)
    DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 7.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9961
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0038
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008077
    • TAT(Tat/SPI): 0.000499
    • LIPO(Sec/SPII): 0.002155
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI: 446953213 NCBI
  • RefSeq: WP_001030469 NCBI
  • UniProt: see SA1597

⊟Protein sequence[edit | edit source]

  • MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPVTFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPKVDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS08995&oldid=74706"
  • This page was last edited on 10 March 2016, at 22:59.
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