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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03530 [old locus tag: SA0615 ]
  • pan locus tag?: SAUPAN002539000
  • symbol: SA_RS03530
  • pan gene symbol?: graS
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03530 [old locus tag: SA0615 ]
  • symbol: SA_RS03530
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 708912..709952
  • length: 1041
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (708912..709952) NCBI
  • BioCyc: SA_RS03530 BioCyc
  • MicrobesOnline: see SA0615

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGG
    ATATTGTTTTTAAACTTGCTTATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATA
    GACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAACAATGATTTTTCTTATATTG
    ACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
    ATTAAACATAAAGATTTAGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATAT
    CGTCAAATCTCAGCGCACAAAGAAAAGGTTGTTGAGCAACAGTTACAATTGAACATGCAT
    GAACAAACCATTACAGAATTTGTGCACGACATAAAAACACCTGTGACAGCCATGAAATTA
    TTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAGGCATTACTATATGAATGGTCTCGT
    ATAAACTCGATGCTGGATACACAGCTGTATATTACTAGATTAGAATCTCAACGCAAAGAT
    ATGTATTTTGATTACGTGTCACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGA
    CATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACTTTAAAGTGGATGATTATGTT
    TATACAGATACAAAATGGTGTCGTATGATTATTAGACAGATTTTGTCAAACGCATTGAAA
    TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTA
    TATATTAAAGACTATGGCAGAGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGA
    GGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTTCAGGTATGGGTCTATATTTA
    GTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
    GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAA
    GTGACAAATTTGTCATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03530 [old locus tag: SA0615 ]
  • symbol: SA_RS03530
  • description: sensor histidine kinase
  • length: 346
  • theoretical pI: 7.34071
  • theoretical MW: 41032.2
  • GRAVY: -0.248555

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • ⊞TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 90.7)
    and 10 more
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 67.3)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 61)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.4)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.4)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 26.3)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 26.3)
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 22.5)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 22.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 14.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 12.9)
  • TheSEED: see SA0615
  • ⊞PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 79.3)
    and 5 more
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 21.1)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 17.8)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 17.4)
    no clan defined TA0956; Thermoplasma acidophilum protein TA0956 (PF11513; HMM-score: 13.7)
    RRM (CL0221) SNU71_RBD; SNU71 RNA binding domain (PF24825; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9995
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008443
    • TAT(Tat/SPI): 0.000153
    • LIPO(Sec/SPII): 0.046968
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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