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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0478 [new locus tag: SACOL_RS02420 ]
  • pan locus tag?: SAUPAN002129000
  • symbol: SACOL0478
  • pan gene symbol?: ssl11
  • synonym:
  • product: superantigen-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0478 [new locus tag: SACOL_RS02420 ]
  • symbol: SACOL0478
  • product: superantigen-like protein
  • replicon: chromosome
  • strand: +
  • coordinates: 480192..480869
  • length: 678
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAATTAAAAAATATTGCTAAAGCAAGTTTAGCACTAGGGATTTTAACAACAGGGATG
    ATTACAACTACTGCTCAGCCAGTAAAAGCAAGTACATTAGAGGTTAGATCACAAGCTACT
    CAAGACTTGAGTGAATATTATAATAGACCGTTCTTTGAGTATACAAATCAGTCAGGATAT
    AAAGAGGAAGGAAAAGTGACGTTTACTCCTAATTATCAACTTATAGATGTAACTTTAACT
    GGGAATGAAAAGCAAAATTTTGGTGAAGATATTTCTAATGTAGATATATTTGTTGTAAGA
    GAAAATTCTGATAGATCTGGTAATACAGCTTCAATTGGTGGTATTACTAAAACAAACGGT
    TCAAATTATATTGATAAAGTAAAAGATGTAAATTTAATAATTACTAAAAACATCGATAGT
    GTTACATCAACGTCAACATCATCTACATATACAATTAATAAAGAAGAAATTTCATTAAAA
    GAACTTGATTTTAAATTAAGAAAGCATTTAATTGATAAACATAACCTTTATAAGACAGAA
    CCTAAAGACAGTAAAATTCGAATTACTATGAAAGATGGTGGGTTCTACACATTTGAATTG
    AATAAAAAGTTACAAACACACCGTATGGGTGATGTTATTGATGGCAGAAATATAGAAAAA
    ATTGAAGTGAATTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0478 [new locus tag: SACOL_RS02420 ]
  • symbol: SACOL0478
  • description: superantigen-like protein
  • length: 225
  • theoretical pI: 9.09262
  • theoretical MW: 25365.5
  • GRAVY: -0.584444

⊟Function[edit | edit source]

  • TIGRFAM:
    phospholamban (TIGR01294; HMM-score: 11.3)
  • TheSEED  :
    • Exotoxin 8
  • ⊞PFAM:
    Ubiquitin (CL0072) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 109.1)
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 93.5)
    and 2 more
    no clan defined DUF7491; Coiled-coil region of unknown function (DUF7491) (PF24319; HMM-score: 15.6)
    PH (CL0266) EbsA; EbsA-like protein (PF17255; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0029
    • Extracellular Score: 0.9969
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.988981
    • TAT(Tat/SPI): 0.007047
    • LIPO(Sec/SPII): 0.002727
    • Cleavage Site: CS pos: 30-31. VKA-ST. Pr: 0.8511
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLSEYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVRENSDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLKELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNIEKIEVNL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SaeR (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)

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