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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA2457 [new locus tag: SA_RS14070 ]
  • pan locus tag?: SAUPAN006409000
  • symbol: SA2457
  • pan gene symbol?: cap1A
  • synonym:
  • product: capsular polysaccharide synthesis protein CapA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2457 [new locus tag: SA_RS14070 ]
  • symbol: SA2457
  • product: capsular polysaccharide synthesis protein CapA
  • replicon: chromosome
  • strand: -
  • coordinates: 2768262..2768924
  • length: 663
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 1125386 NCBI
  • RefSeq: NP_375783 NCBI
  • BioCyc: see SA_RS14070
  • MicrobesOnline: 104809 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGAAAAGTTTGATTTAGTAAAACTATTAAATATTCTAAAGAAGAATATTAAATTA
    TTGCTTATTTTACCGGCAATATGTCTTGTAGTAAGTACGGCATTAACCTTCTTTGTTATG
    CCTGACAAATATACTGCTTCTACTCAAATACTAGTGAACATGAAAAAGTCCTCAAGTGAT
    TTAGCTTTCCAAAATGTTCAAAGTAGTTTGCAGTCTGTTAATACATATACGGAAATTATC
    AAAAGTCCAAGAATTCTAGATAAAGTATCTAGAGAGTTTGATGGTCAGTATTCAACAGCT
    GAGTTGAATTCGTTTTTAAAAGTAACCAATCAAACAAACTCTCAGATTATTACTGTATCA
    GTTACAACTGGGAATAAATCTGAATCTGACAAAATTGTTAATAGAATATCTAAAGTTTTT
    GCACATGATATGCCTAAAATTATGAGTGTTGATAATGTTACGATACTTTCCTCAGCACAT
    GATAATGCTGTAAAAGTATCTCCAATAGTATCTGTCAACTTAGTGATCAGCATTATTGTT
    GGTATTGTTTTAGCAATATTGATTATTTTCTTAAAAGAATTATTAGATAAGCGTATTAAG
    ACAGAAGAAGATGTTGAATCGCAACTAGGATTACCTATTTTAGGTTCAATACAAAAATTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2457 [new locus tag: SA_RS14070 ]
  • symbol: SA2457
  • description: capsular polysaccharide synthesis protein CapA
  • length: 220
  • theoretical pI: 9.83197
  • theoretical MW: 24397.6
  • GRAVY: 0.285

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polysaccharide export protein, MPA1 family (TIGR01006; HMM-score: 239)
    and 4 more
    chain length determinant protein EpsF (TIGR03017; HMM-score: 57.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 40.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (TIGR03007; HMM-score: 29)
    NDMA-dependent methanol dehydrogenase (TIGR04266; EC 1.1.99.37; HMM-score: 15.2)
  • ⊞TheSEED  :
    • Tyrosine-protein kinase transmembrane modulator EpsC
    Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis  Tyrosine-protein kinase transmembrane modulator EpsC
    and 1 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Tyrosine-protein kinase transmembrane modulator EpsC
  • ⊞PFAM:
    no clan defined Wzz; Chain length determinant protein (PF02706; HMM-score: 85.8)
    and 4 more
    GNVR; G-rich domain on putative tyrosine kinase (PF13807; HMM-score: 18.6)
    DUF2975; Protein of unknown function (DUF2975) (PF11188; HMM-score: 16)
    Amnionless; Amnionless (PF14828; HMM-score: 10.7)
    DUF3176; Protein of unknown function (DUF3176) (PF11374; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9949
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.005
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.138123
    • TAT(Tat/SPI): 0.001737
    • LIPO(Sec/SPII): 0.020944
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI: 15928250 NCBI
  • RefSeq: NP_375783 NCBI
  • UniProt: P63859 UniProt

⊟Protein sequence[edit | edit source]

  • MKEKFDLVKLLNILKKNIKLLLILPAICLVVSTALTFFVMPDKYTASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAELNSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAHDNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQKF

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SA2455 < SA2456 < SA2457

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA2457&oldid=57676"
  • This page was last edited on 10 March 2016, at 13:51.
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